Development of in vivo mitochondrial gene delivery system and validation of mitochondrial exogenous gene expression in skeletal muscle
Project/Area Number |
23680053
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Single-year Grants |
Research Field |
Biomedical engineering/Biological material science
|
Research Institution | Hokkaido University |
Principal Investigator |
YAMADA Yuma 北海道大学, 薬学研究科(研究院), 助教 (60451431)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2011: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
|
Keywords | 薬学 / ミトコンドリア / 遺伝子送達 / ナノテクノロジー / 細胞・動物 / 遺伝子治療 / イメージング / バイオテクノロジー |
Research Abstract |
Mitochondrial genetic disorders are a major cause of mitochondrial diseases including mitochondrial inherited diseases, neurodegenerative diseases and diabetes. It is therefore likely that mitochondrial gene therapy will be useful for the treatment of such diseases. To achieve such an innovative therapy, successful mitochondrial exogenous gene expression, two independent processes, i.e., "development of in vivo mitochondrial gene delivery system" and "construction of DNA vector to achieve mitochondrial gene expression" are required. In this study, we report on successful mitochondrial gene delivery in skeletal muscle using hydrodynamic limb vein (HLV) injection. Moreover, we could construct the DNA vector which can express on mitochondria through hydrodynamic gene delivery. Our mitochondrial exogenous gene expression system promises to be a useful technique for mitochondrial gene therapy and research regarding mitochondrial molecular biology.
|
Report
(4 results)
Research Products
(85 results)
-
-
-
-
-
-
-
-
-
[Journal Article] A Lipid Envelop-type Nano Particle Incorporating a Multifunctional Peptide: GALA for the Systemic siRNA Delivery to the Pulmonary Endothelium.2013
Author(s)
Kusumoto K, Akita H, Ishitsuka T, Matsumoto Y, Nomoto T, Furukawa R, El-Sayed A, Hatakeyama H, Kajimoto K, Yamada Y, Kataoka K, Harashima H.
-
Journal Title
ACS Nano.
Volume: 7
Issue: 9
Pages: 7534-7541
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] 応用が拡がるDDS2013
Author(s)
秋田英万、山田勇磨、畠山浩人、林泰弘、原島秀吉
Total Pages
578
Publisher
株式会社エヌ・ティー・エス
Related Report
-
[Book] 応用が拡がるDDS.2013
Author(s)
秋田英万、山田勇磨、畠山浩人、林泰弘、原島秀吉.
Total Pages
578
Publisher
株式会社エヌ・ティー・エス
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-