Ultrafast laser nanosurgery of cells and antigen against infectious diseases

• Project/Area Number: 23680058
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Medical systems
• Research Institution: Keio University
• Principal Investigator: TERAKAWA Mitsuhiro 慶應義塾大学, 理工学部, 講師 (60580090)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 低侵襲治療システム / レーザー医療 / ナノサージェリー / 細胞膜穿孔 / ドラッグデリバリー / フェムト秒レーザー / 誘電体粒子

Research Abstract

Cell membrane surgery by femtosecond laser-excited enhanced optical field was demonstrated. Simultaneous perforation of multiple cells was demonstrated by using a single pulse of unfocused femtosecond laser with cell selectivity. Combined method of nanoscale-laser processing with limited heat affected zone and high-throughput treatment by simultaneous excitation of multiple particles was realized.

Research Products

• [Journal Article] Evaluation of parameters influencing the molecular delivery by biodegradable microsphere-mediated perforation using femtosecond laser (2014)
  • Author(s): T. Mitsuhashi, M. Terakawa
  • Journal Title: Journal of Biomedical Optics Volume: 19 Issue: 1 Pages: 015003-015003
  • DOI: 10.1117/1.jbo.19.1.015003
  • Peer Reviewed
• [Journal Article] Near-infrared femtosecond laser-triggered nano- perforation of hollow microcapsules (2013)
  • Author(s): M. Terakawa, T. Mitsuhashi, T. Shinohara, H. Shimizu
  • Journal Title: Optics Express Volume: 21 Issue: 10 Pages: 12604-12610
  • DOI: 10.1364/oe.21.012604
  • Peer Reviewed
• [Journal Article] In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 fs near-infrared laser pulse (2012)
  • Author(s): M. Terakawa, Y. Tsunoi, T. Mitsuhashi
  • Journal Title: International Journal of Nanomedicine Volume: 7 Pages: 2653-2660
  • DOI: 10.2147/ijn.s31768
  • Peer Reviewed
• [Journal Article] Biodegradable microsphere mediated membrane permeabili- zation and transfection using femtosecond laser (2012)
  • Author(s): M. Terakawa
  • Journal Title: Proceedings of CLMS Volume: 8-4 Pages: 92-93
• [Journal Article] Biodegradable microsphere mediated membrane permeabilization and transfection using femtosecond laser (2012)
  • Author(s): M. Terakawa
  • Journal Title: Proc. CLMS Volume: 8-4 Pages: 92-93
• [Journal Article] Dielectric microsphere mediated transfection using femtosecond laser (2011)
  • Author(s): M. Terakawa, Y. Tanaka
  • Journal Title: Optics Letters Volume: 36 Issue: 15 Pages: 2877-2879
  • DOI: 10.1364/ol.36.002877
  • Peer Reviewed
• [Presentation] Microfluidic perforation system using femtosecond laser and biodegradable microspheres (2014)
  • Author(s): 三橋龍樹
  • Organizer: International Workshop on the Fabrication and Application of Microstructured Optical Devices
  • Place of Presentation: 横浜
  • Year and Date: 2014-02-28
• [Presentation] Femtosecond laser processing for biomedical applications (2014)
  • Author(s): 寺川光洋
  • Organizer: International Workshop on the Fabrication and Application of Microstructured Optical Devices
  • Place of Presentation: 横浜
  • Year and Date: 2014-02-27
• [Presentation] Biodegradable microsphere-mediated perforation using low-intensity femtosecond laser pulses for microfluidic perforation system (2014)
  • Author(s): 三橋龍樹
  • Organizer: SPIE Photonics West 2014
  • Place of Presentation: サンフランシスコ(米国)
  • Year and Date: 2014-02-02
• [Presentation] Biodegradable microsphere- mediated perforation using low-intensity femtosecond laser pulses for microfluidic perforation system (2014)
  • Author(s): T. Mitsuhashi, M. Terakawa
  • Organizer: SPIE Photonics West
  • Place of Presentation: 米国、サンフランシスコ
• [Presentation] Femtosecond laser- triggered nanoperforation of polymer hollow microsphere (2013)
  • Author(s): 寺川光洋
  • Organizer: 12th International Conference on Laser Ablation (COLA 2013)
  • Place of Presentation: イスキア(イタリア)
  • Year and Date: 2013-10-07
• [Presentation] フェムト秒レーザ励起光増強場によるドラッグデリバリー (2013)
  • Author(s): 寺川光洋
  • Organizer: 分子デリバリー研究会
  • Place of Presentation: 東京
  • Year and Date: 2013-09-24
• [Presentation] Size-dependent nanoparticle delivery by biodegradable microsphere-mediated perforation using femtosecond laser (2013)
  • Author(s): 三橋龍樹
  • Organizer: SPIE Optics + Photonics 2013
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2013-08-26
• [Presentation] Femtosecond laser nanoablation by enhanced optical field (2013)
  • Author(s): 寺川光洋
  • Organizer: The 6th International Congress on Laser Advanced Materials Processing (LAMP 2013)
  • Place of Presentation: 新潟
  • Year and Date: 2013-07-25
• [Presentation] 生分解性ポリマー微粒子を用いたレーザー薬剤導入における導入分子サイズの検討 (2013)
  • Author(s): 三橋龍樹
  • Organizer: 第29回日本DDS学会学術会議
  • Place of Presentation: 京都
  • Year and Date: 2013-07-04
• [Presentation] Femtosecond laser-triggered local destruction of polymer microcapsules for drug delivery (2013)
  • Author(s): 寺川光洋
  • Organizer: European Conferences on Biomedical Optics (ECBO 2013)
  • Place of Presentation: ミュンヘン(ドイツ)
  • Year and Date: 2013-05-14
• [Presentation] 誘電体粒子レンズを用いたフェムト秒レーザ細胞プロセシングとドラッグデリバリーへの応用 (2013)
  • Author(s): 寺川光洋
  • Organizer: 第79回レーザ加工学会講演会
  • Place of Presentation: 大阪
  • Year and Date: 2013-05-07
• [Presentation] 多微粒子レンズ集光場によるフェムト秒レーザ細胞プロセシング (2013)
  • Author(s): 寺川光洋
  • Organizer: 電気学会光・量子デバイス研究会
  • Place of Presentation: 松江
  • Year and Date: 2013-03-08
• [Presentation] Biodegradable microsphere mediated perforation of cells using femtosecond laser for theranos- tics (2013)
  • Author(s): 寺川光洋
  • Organizer: SPIE Photonics West 2013
  • Place of Presentation: サンフランシスコ(米国)
  • Year and Date: 2013-02-03
  • Invited
• [Presentation] Femtosecond laser-triggered nanoperforation of polymer hollow microsphere (2013)
  • Author(s): M. Terakawa, T. Mitsuhashi, T. Shinohara
  • Organizer: 12th International Conference on Laser Ablation (COLA)
  • Place of Presentation: イタリア、イスキア
• [Presentation] Size-dependent nanoparticle delivery by biodegradable microsphere-mediated perforation using femtosecond laser (2013)
  • Author(s): T. Mitsuhashi, M. Terakawa
  • Organizer: SPIE Optics + Photonics (2013)
  • Place of Presentation: 米国、サンディエゴ
• [Presentation] Femtosecond laser-triggered local destruction of polymer microcapsules for drug delivery (2013)
  • Author(s): M. Terakawa
  • Organizer: European Conferences on Biomedical Optics (ECBO)
  • Place of Presentation: ドイツ、ミュンヘン
• [Presentation] Enhanced cell membrane permeability by using multi-microsphere lens focusing of burst mode of low-intensity 80 MHz femtosecond lasers (2012)
  • Author(s): 三橋龍樹
  • Organizer: 第73回応用物理学会学術講演会
  • Place of Presentation: 松山
  • Year and Date: 2012-09-12
• [Presentation] Dielectric microsphere mediated cell perforation using a single shot femtosecond laser pulse (2012)
  • Author(s): 寺川光洋
  • Organizer: The 8th Asia Pacific Laser Symposium (APLS 2012)
  • Place of Presentation: 黄山(中国)
  • Year and Date: 2012-05-28
• [Presentation] Biodegradable microsphere mediated membrane permeabilization and transfection using femtosecond laser (2012)
  • Author(s): 寺川光洋
  • Organizer: Conference on Laser Surgery and Medicine 2012 (CLMS2012)
  • Place of Presentation: 横浜
  • Year and Date: 2012-04-27
• [Presentation] フェムト秒レーザの多微粒子レンズ集光場による細胞膜の単一パルスプロセッシング (2012)
  • Author(s): 寺川光洋
  • Organizer: 講演奨励賞受賞記念講演,第59回応用物理学関係連合講演会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-16
• [Presentation] フェムト秒レーザの多微粒子レンズ集光場による細胞膜の単一パルスプロセッシング (2012)
  • Author(s): 寺川光洋
  • Organizer: 第59回応用物理学関係連合講演会
  • Place of Presentation: 早稲田大学(東京都)(講演奨励賞受賞記念講演)
  • Year and Date: 2012-03-16
• [Presentation] 低強度多パルスフェムト秒レーザーの多微粒子レンズ集光場を用いた細胞への物質送達 (2012)
  • Author(s): 三橋龍樹
  • Organizer: 第12回レーザー学会東京支部研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-06
• [Presentation] 低強度多パルスフェムト秒レーザーの多微粒子レンズ集光場を用いた細胞への物質送達 (2012)
  • Author(s): 三橋龍樹、寺川光洋
  • Organizer: 第12回レーザー学会東京支部研究会
  • Place of Presentation: 東海大学高輪キャンパス(東京都)
  • Year and Date: 2012-03-06
• [Presentation] Biodegradable microsphere mediated cell perforation using femtosecond laser pulse (2012)
  • Author(s): 寺川光洋
  • Organizer: SPIE Photonics West 2012
  • Place of Presentation: サンフランシスコ(米国)
  • Year and Date: 2012-01-22
• [Presentation] Biodegradable microsphere mediated cell perforation using femtosecond laser pulse (2012)
  • Author(s): Mitsuhiro Terakawa, Tatsuki Mitsuhashi
  • Organizer: SPIE Photonics West 2012 (LASE)
  • Place of Presentation: The Moscone Center, San Francisco, USA
  • Year and Date: 2012-01-22
• [Presentation] Polystyrene microsphere mediated cell perforation using a single femtosecond laser pulse (2011)
  • Author(s): 寺川光洋
  • Organizer: 11th International Conference on Laser Ablation
  • Place of Presentation: プラヤデルカルメン(メキシコ)
  • Year and Date: 2011-11-18
• [Presentation] Polystyrene microsphere mediated cell perforation using a single femtosecond laser pulse (2011)
  • Author(s): Mitsuhiro Terakawa, Yuto Tanaka
  • Organizer: 11th International Conference on Laser Ablation
  • Place of Presentation: Playa del Carmen, Mexico
  • Year and Date: 2011-11-18
• [Presentation] フェムト秒レーザの多微粒子レンズ集光場による細胞膜の単一パルスプロセッシング (2011)
  • Author(s): 寺川光洋
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形
  • Year and Date: 2011-08-31
• [Presentation] フェムト秒レーザの多微粒子レンズ集光場による細胞膜の単一パルスプロセッシング (2011)
  • Author(s): 寺川光洋
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形大学(山形県)
  • Year and Date: 2011-08-31
• [Presentation] Biodegradable microsphere mediated membrane permeabilization and transfection using femtosecond laser
  • Author(s): M. Terakawa
  • Organizer: Conference on Laser Surgery and Medicine 2012
  • Place of Presentation: 横浜
• [Presentation] Dielectric microsphere mediated cell perforation using a single shot femtosecond laser pulse
  • Author(s): M. Terakawa
  • Organizer: The 8th Asia Pacific Laser Symposium
  • Place of Presentation: 黄山（中国）
• [Presentation] Enhanced cell membrane permeability by using multi-microsphere lens focusing of burst mode of low-intensity 80 MHz femtosecond lasers
  • Author(s): T. Mitsuhashi
  • Organizer: 第73回応用物理学会学術講演会
  • Place of Presentation: 松山
• [Presentation] Biodegradable microsphere mediated perforation of cells using femtosecond laser for theranostics
  • Author(s): M. Terakawa
  • Organizer: SPIE Photonics West
  • Place of Presentation: サンフランシスコ（米国）
  • Invited
• [Presentation] 多微粒子レンズ集光場によるフェムト秒レーザ細胞プロセシング
  • Author(s): 寺川光洋
  • Organizer: 電気学会 光・量子デバイス研究会
  • Place of Presentation: 松江
• [Remarks] "Femtosecond laser pulses for smart drug delivery,"Mitsuhiro Terakawa, 2014年3月21日, SPIE Newsroom. DOI : 10.1117/ 2.1201403.005377
• [Patent(Industrial Property Rights)] レーザーを用いた薬剤放出方法 (2013)
  • Inventor(s): 寺川光洋
  • Industrial Property Rights Holder: 慶應義塾大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-046732
  • Filing Date: 2013-03-08
  • Overseas