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Microbial production of unusual biopolyesters

Research Project

Project/Area Number 23681015
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Environmental technology/Environmental materials
Research InstitutionHokkaido University

Principal Investigator

MATSUMOTO KENICHIRO  北海道大学, 工学(系)研究科(研究院), 准教授 (80360642)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2011: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Keywordsバイオベースプラスチック / バイオテクノロジー / バイオポリマー / 微生物合成 / バイオマス
Research Abstract

Bacterial polyhydroxyalkanoates (PHAs) are intracellular storage material, which can be used as a biobased plastic. The most common PHA is P(3-hydroxybutyrate), which has been found in a variety of bacteria and produced with good yield. However, the brittleness of the polymer has limited the practical use of the material. This study addressed this problem by using engineered polymer synthetic systems, which are capable synthesizing unusual PHAs. For examples, the isotactic P(2-hydroxybutyrate) was efficiently produced using engineered Escherichia coli. The microbial process had an advantage over the chemical polymerization in that the enzymatic polymerization is capable of synthesizing isotactic polymer from inexpensive racemic precursor. The polyester exhibited excellent transparency and flexibility, which differed from conventional PHAs. The glycolate-based polyester also produced using E. coli. This was the first case for the intracellular polymerization of glycolate.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (36 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (26 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] Enhanced production of poly(lactate-co-3-hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter2014

    • Author(s)
      John Masani NDUKO, Ken'ichiro MATSUMOTO, Toshihiko OOI, Seiichi TAGUCHI
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: Vol.98 Pages: 2453-60

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced production of poly(lactate-co-3-hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter2014

    • Author(s)
      Nduko JM, Matsumoto K, Ooi T, Taguchi S
    • Journal Title

      Appl Microbiol Biotechnol.

      Volume: 98 Issue: 6 Pages: 2453-2460

    • DOI

      10.1007/s00253-013-5401-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme2013

    • Author(s)
      John Masani NDUKO, Ken'ichiro MATSUMOTO, Toshihiko OOI, Seiichi TAGUCHI
    • Journal Title

      Metab. Eng.

      Volume: Vol.15 Pages: 159-166

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] One-pot microbial production, mechanical properties and enzymatic degradation of isotactic P[(R)-2-hydroxybutyrate] and its copolymer with (R)-lactate2013

    • Author(s)
      Ken'ichiro MATSUMOTO, Satsuki TERAI, Ayako ISHIYAMA, Jian SUN, Taizo KABE, Yuyang SONG, John NDUKO, Tadahisa IWATA, Seiichi TAGUCHI
    • Journal Title

      Biomacromolecules

      Volume: Vol.14 Pages: 1913-1918

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3- hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme2013

    • Author(s)
      JM. Nduko, K. Matsumoto, T. Ooi, S. Taguchi
    • Journal Title

      Metab. Eng

      Volume: vol.15 Pages: 159-166

    • DOI

      10.1016/j.ymben.2012.11.007

    • NAID

      120005228684

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] One-pot microbial production, mechanical properties and enzymatic degradation of isotactic P[(R)-2-hydroxybutyrate] and its copolymer with (R)-lactate2013

    • Author(s)
      Ken'ichiro Matsumoto
    • Journal Title

      Biomacromolecules

      Volume: 未定

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme and metabolic engineering for the production of novel polymers: crossover of biological and chemical processes2013

    • Author(s)
      Ken'ichiro Matsumoto
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 未定 Issue: 6 Pages: 1054-1060

    • DOI

      10.1016/j.copbio.2013.02.021

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase2011

    • Author(s)
      Ken'ichiro MATSUMOTO, Ayako ISHIYAMA, Kohei SAKAI, Tetsufumi SHIBA, Seiichi TAGUCHI
    • Journal Title

      J. Biotechnol.

      Volume: Vol.156(3) Pages: 214-217

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis of glycolate-basea polyesters containing medium-chainlength2011

    • Author(s)
      Ken'ichiro Matsumoto, Ayako Ishiyama, Kohei Sakai, Tetsufumi
    • Journal Title

      Journal of Biotechnology

      Volume: 156 Issue: 3 Pages: 214-217

    • DOI

      10.1016/j.jbiotec.2011.07.040

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 微生物産生ポリ[(R-)-ラクテート-co--(R)-2-ヒドロキシブチレート]の結晶性および熱的性質2014

    • Author(s)
      加部泰三、松本謙一郎、引間孝明、丸林弘典、髙田昌樹、田口精一、岩田忠久
    • Organizer
      第63回高分子学会年次大会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-05-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物を利用したキラルP(2-ヒドロキシ酪酸)の合成とポリマー解析2014

    • Author(s)
      三宅政裕、松本謙一郎、寺井彩月、加部泰三、岩田忠久、田口精一
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] バイオマスから高効率で乳酸プラスチックを生産する微生物工場の開発とポリマー物性評価2014

    • Author(s)
      松本謙一郎、前田理久、田口精一
    • Organizer
      農芸化学会
    • Place of Presentation
      明治大学(川崎市多摩区)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 微生物を利用したキラルP(2-ヒドロキシ酪酸)の合成とポリマー解析2014

    • Author(s)
      三宅政裕、松本謙一郎、寺井彩月、加部泰三、岩田忠久、田口精一
    • Organizer
      農芸化学会
    • Place of Presentation
      明治大学(川崎市多摩区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficient production of poly(lactate-co-3-hydroxybutyrate) using hemicellulose-derived sugar, xylose, in engineered Escherichia coli overexpressing a galactitol transporter2013

    • Author(s)
      John Masani Nduko, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
    • Organizer
      Frontier Chemistry Center International Symposium 2013 "Advanced Materials Science"
    • Place of Presentation
      Sapporo
    • Year and Date
      2013-12-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Microbial synthesis of isotactic P(2-hydroxybutyrate) from racemic precursor and its property analysis2013

    • Author(s)
      Ken'ichiro Matsumoto, Jian Sun, Seiichi Taguchi
    • Organizer
      The 13th Pacific Polymer Conference
    • Place of Presentation
      Taiwan
    • Year and Date
      2013-11-19
    • Related Report
      2013 Final Research Report
  • [Presentation] ポリヒドロキシアルカン酸重合酵素の機能改変によるポリマー構造制御2013

    • Author(s)
      松本謙一郎、越智杏奈、大場貴史、高谷真宏、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖を利用した組換え大腸菌でのグリコール酸ベースポリマーの生産2013

    • Author(s)
      斯波哲史、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 不飽和モノマーを導入した2-ヒドロキシブタン酸ベースポリマーの生合成とその応用2013

    • Author(s)
      崔允圭、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 2-ヒドロキシブタン酸ベースポリマーの微生物合成とその物性解析2013

    • Author(s)
      三宅政裕、寺井彩月、松本謙一郎、加藤泰三、岩田忠久、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 改変型重合酵素による2-ヒドロキシブタン酸ベースポリマーの生合成と物性解析2013

    • Author(s)
      青木駿介、大場貴史、越智杏奈、松本謙一郎、田口精一
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 改変型重合酵素による2-ヒドロキシブタン酸ベースポリマーの生合成と物性解析2013

    • Author(s)
      青木駿介
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 2-ヒドロキシブタン酸ベース新規バイオプラスチックの微生物合成と物性解析2012

    • Author(s)
      寺井彩月、石山絢子、松本謙一郎、田口精一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Expanding microbial polyesters : syntheses and properties2012

    • Author(s)
      Ken'ichiro Matsumoto, Seiichi Taguchi
    • Organizer
      ISBP
    • Place of Presentation
      Australia
    • Year and Date
      2012-10-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Efficient bioconversion of lignocellulosic biomass-derived sugars into poly(lactate-co-3-hydroxybutyrate) by metabolically engineered Escherichia coli2012

    • Author(s)
      John Masani Nduko, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
    • Organizer
      15th IBS
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2012-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Microbial plastic factory : Synthesis and properties of the new lactate-based and related biopolymers2012

    • Author(s)
      John Masani Nduko, Ken'ichiro Matsumoto, Yuyang Song, Seiichi Taguchi
    • Organizer
      The 244th ACS National Meeting & Exposition
    • Place of Presentation
      Philadelphia, Pennsylvania
    • Year and Date
      2012-08-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Biosynthesis and properties of advanced biopolymers containing lactate by metabolically engineered bacteria2012

    • Author(s)
      Nduko John Masani, Ken'ichiro Matsumoto, Seiichi Taguchi
    • Organizer
      The 244th ACS National Meeting & Exposition
    • Place of Presentation
      Philadelphia, Pennsylvania
    • Year and Date
      2012-08-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Lactate-based polyesters production by recombinant bacteria using lignocellulosic biomass sugars as carbon sources2012

    • Author(s)
      John Masani Nduko,Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
    • Organizer
      Japan-Chica-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Kanazawa
    • Year and Date
      2012-05-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 組換え大腸菌による乳酸およびグリコール酸ベース新奇バイオプラスチックの生合成と物性解析2012

    • Author(s)
      斯波哲史,石山絢子,松本謙一郎,田口精一
    • Organizer
      農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Microbial synthesis of chiral polymers from cellulosic biomass and racemic compounds2012

    • Author(s)
      Ken'ichiro Matsumoto
    • Organizer
      International Symposium on Catalytic Biomass Conversion
    • Place of Presentation
      Hokkaido University(札幌)(招待講演)
    • Year and Date
      2012-02-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 組換え大腸菌によるグリコール酸ベースポリマーの生合成2012

    • Author(s)
      石山絢子,斯波哲史,松本謙一郎,田口精一
    • Organizer
      高分子学会北海道支部研究発表会
    • Place of Presentation
      札幌
    • Year and Date
      2012-01-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Expanding microbial polyesters: syntheses and properties2012

    • Author(s)
      Ken'ichiro Matsumoto
    • Organizer
      International Symposium on Biological Polymers
    • Place of Presentation
      Pullman Reef Hotel (Australia)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 2-ヒドロキシブタン酸ベース新規バイオプラスチックの微生物合成と物性解析2012

    • Author(s)
      寺井彩月
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 乳酸およびグリコール酸ベース新奇バイオプラスチックの微生物生産2011

    • Author(s)
      斯波哲史、石山絢子、松本謙一郎、田口精一
    • Organizer
      農芸化学会北海道支部学術講演会
    • Place of Presentation
      札幌
    • Year and Date
      2011-11-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Advanced microbial polymer factory for incorporating new monomers : lactate, glycolate and 2-hydroxybutyrate2011

    • Author(s)
      Ken'ichiro Matsumoto, Seiichi Taguchi
    • Organizer
      The 3rd International Conference on Bio-based polymers 2011
    • Place of Presentation
      Peking University(北京)
    • Year and Date
      2011-10-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Production of lactate-based polyesters from xylose in Escherichia coli2011

    • Author(s)
      John Masani Nduko, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-27
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.eng.hokudai.ac.jp/labo/seika/TOP.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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