Development of methods for controlling fluid dynamics of single molecules using gating nanopore devices
| Project/Area Number |
23681031
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Keywords | ゲーティングナノポア / ナノポア / 単分子 / 流動制御 / 生体分子 / １分子科学 / １分子技術 / ＤＮＡシークエンサー / 単分子流動 / １分子解析技術 / トンネル伝導 / DNAシーケンサー / トンネル電流 / 1分子科学 / 1分子技術 |
|---|
| Research Abstract |
The method, which is used to control the translocation speed of a single DNA and RNA molecule, is a key technology used for identifying base molecules with high accuracy and throughput. We developed surrounding gate nanopores, which consist of a gating nanopore and gate electrode. We demonstrated the calculated gate voltage dependence of the DNA translocation velocity, using theoretical simulations. In addition, in an effort to demonstrate the control method for the translocation speed using gate voltage, we fabricated a surrounding gate nanopore with a diameter of 20 nm. The translocation time was found to be 35 ms without a gate voltage. On applying a gate voltage of -0.5 V, we obtained slower translocation times of the order of approximately 350 ms, which is caused by the upward electro-osmotic flow. Thus, we demonstrated that surrounding gate nanopores can control the translocation speed of single DNA and RNA molecules using gate voltages.
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Report
(4 results)
Research Products
(56 results)
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[Presentation] １分子科学の未来戦略
Author(s)
谷口 正輝
Organizer
大阪大学 産業科学研究所 第 69 回学術講演会
Place of Presentation
大阪大学産業科学研究所 管理棟講堂
Related Report
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