| Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Research Abstract |
The method, which is used to control the translocation speed of a single DNA and RNA molecule, is a key technology used for identifying base molecules with high accuracy and throughput. We developed surrounding gate nanopores, which consist of a gating nanopore and gate electrode. We demonstrated the calculated gate voltage dependence of the DNA translocation velocity, using theoretical simulations. In addition, in an effort to demonstrate the control method for the translocation speed using gate voltage, we fabricated a surrounding gate nanopore with a diameter of 20 nm. The translocation time was found to be 35 ms without a gate voltage. On applying a gate voltage of -0.5 V, we obtained slower translocation times of the order of approximately 350 ms, which is caused by the upward electro-osmotic flow. Thus, we demonstrated that surrounding gate nanopores can control the translocation speed of single DNA and RNA molecules using gate voltages.
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