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Understanding mechanisms of evolution of chordates through genome editing technologies

Research Project

Project/Area Number 23681039
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Genome biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

SASAKURA Yasunori  筑波大学, 生命環境系, 教授 (10400649)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Keywords脊索動物 / 進化 / 遺伝子改変 / ノックアウト / カタユウレイボヤ / 脊椎動物 / 発生・分化 / Hox / トランスポゾン / ゲノム編集 / ゲノム / 遺伝学 / 発生
Outline of Final Research Achievements

This study aimed to establish molecular and genetic technologies for investigating gene functions in the chordate Ciona intestinalis for elucidating mechanisms of the evolution of chordates. Through transposon-mediated transgenesis and gene knockouts, this study revealed functions of various genes that are conserved among chordates in this ascidian. Hox10 is essential for forming the intestine by regulating the migration of endodermal cells. Gonadotropin-releasing hormones expressed at the larval stage are involved in the regulation of metamorphosis of Ciona. New mechanisms of cell cycle regulation has been revealed that are mediated by conserved, developmentally relevant transcription factors. These new findings provide us new insights for deepen the knowledge about the evolution of chordates and ascidians.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (49 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results,  Acknowledgement Compliant: 6 results,  Open Access: 2 results) Presentation (29 results) (of which Invited: 5 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Metamorphosis in solitary ascidians.2015

    • Author(s)
      Karaiskou A, Swalla BJ, Sasakura Y, Chambon JP.
    • Journal Title

      Genesis

      Volume: 53 Issue: 1 Pages: 34-47

    • DOI

      10.1002/dvg.22824

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sustained heterozygosity across a self-incompatibility locus in an inbred ascidian..2015

    • Author(s)
      Satou Y, Hirayama K, Mita K, Fujie M, Chiba S, Yoshida R, Endo T, Sasakura Y, Inaba K, Satoh N.
    • Journal Title

      Molecular Biology and Evolution

      Volume: 32 Issue: 1 Pages: 81-90

    • DOI

      10.1093/molbev/msu268

    • NAID

      120005619799

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine2015

    • Author(s)
      Kawai N, Ogura Y, Ikuta T, Saiga H, Hamada M, Sakuma T, Yamamoto T, Satoh N, Sasakura Y
    • Journal Title

      Developmental Biology

      Volume: 印刷中 Issue: 1 Pages: 43-56

    • DOI

      10.1016/j.ydbio.2015.03.018

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis.2014

    • Author(s)
      Sasaki H, Yoshida K, Hozumi A, Sasakura Y.
    • Journal Title

      Development Growth and Differentiation

      Volume: 56 Issue: 7 Pages: 499-510

    • DOI

      10.1111/dgd.12149

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Transposon-mediated targeted and specific knockdown of maternally expressed transcripts in the ascidian Ciona intestinalis2014

    • Author(s)
      Takako Iitsuka, Kaoru Mita, Akiko Hozumi, Mayuko Hamada, Nori Satoh and Yasunori Sasakura
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 5050-5050

    • DOI

      10.1038/srep05050

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases.2014

    • Author(s)
      Yoshida K, Treen N, Hozumi A, Sakuma T, Yamamoto T, Sasakura Y.
    • Journal Title

      Genesis

      Volume: in press Issue: 5 Pages: 431-439

    • DOI

      10.1002/dvg.22770

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tissue-specific and ubiquitous gene knockouts in Ciona by electroporating TALENs provide new approaches to investigate gene functions2014

    • Author(s)
      Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T., Sasakura, Y.
    • Journal Title

      Development

      Volume: 141 Issue: 2 Pages: 481-487

    • DOI

      10.1242/dev.099572

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ascidians as excellent models for studying cellular events in the chordate body plan.2014

    • Author(s)
      Ogura Y, Sasakura Y.
    • Journal Title

      Biological Bulletin

      Volume: 224 Pages: 227-236

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element.2013

    • Author(s)
      Akiko Hozumi
    • Journal Title

      Developmental Dynamics

      Volume: 242 Issue: 1 Pages: 30-43

    • DOI

      10.1002/dvdy.23891

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome2013

    • Author(s)
      Akiko Hozumi
    • Journal Title

      Developmental Biology

      Volume: 375 Issue: 1 Pages: 79-91

    • DOI

      10.1016/j.ydbio.2012.12.012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retinoic acid-driven Hoxl is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis.2012

    • Author(s)
      Yasunori Sasakura
    • Journal Title

      Development

      Volume: (印刷中) Issue: 12 Pages: 2156-2160

    • DOI

      10.1242/dev.080234

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases.2012

    • Author(s)
      Kawai N, Ochiai H, Sakuma T, Yamada L, Sawada H, Yamamoto T, Sasakura Y.
    • Journal Title

      Development, Growth & Differentiation

      Volume: 54 Issue: 5 Pages: 535-545

    • DOI

      10.1111/j.1440-169x.2012.01355.x

    • NAID

      10031136923

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Large-scale infection of the ascidian Ciona intestinalis by the gregarine Lankesteria ascidiae in an inland culture system.2012

    • Author(s)
      Kaoru Mita
    • Journal Title

      Diseases of Aquatic Organisms

      Volume: 101 Issue: 3 Pages: 185-195

    • DOI

      10.3354/dao02534

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ascidians as excellent chordate models for studying the development of the nervous system during embryogenesis and metamorphosis.2012

    • Author(s)
      Yasunori Sasakura
    • Journal Title

      Developmental Growth and Differentiation

      Volume: 54 Issue: 3 Pages: 420-437

    • DOI

      10.1111/j.1440-169x.2012.01343.x

    • NAID

      10031136914

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monoaminergic modulation of photoreception in ascidian : Evidence for a proto-hypothalamo-retinal territory2012

    • Author(s)
      Florian Razy-Krajka, Euan R. Brown, Takeo Horie, Jacques Callebert, Yasunori Sasakura, Jean-Stéphane Joly, Takehiro G. Kusakabe, and Philippe Vernier
    • Journal Title

      BMC Biology

      Volume: 10 Issue: 1 Pages: 45-45

    • DOI

      10.1186/1741-7007-10-45

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of enhancer detection lines expressing GFP in the gut of the ascidian Ciona intestinalis2012

    • Author(s)
      Reiko Yoshida, Yasunori Sasakura
    • Journal Title

      Zoological Science

      Volume: 29 Issue: 1 Pages: 11-20

    • DOI

      10.2108/zsj.29.11

    • NAID

      210000178145

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 新規母性mRNA特異的ノックダウン法(MASK法)を用いた脊索動物ホヤにおける母性mRNAの局在メカニズムの解明2014

    • Author(s)
      佐藤 瑛生、飯塚 貴子、笹倉 靖徳
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] マスク法によるカタユウレイボヤ母性mRNAの特異的機能阻害2014

    • Author(s)
      笹倉 靖徳
    • Organizer
      ホヤ研究会2014
    • Place of Presentation
      筑波大学東京キャンパス(東京都文京区)
    • Year and Date
      2014-10-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Hox1による内柱の前後パターン形成とその意義2014

    • Author(s)
      吉田 慶太
    • Organizer
      ホヤ研究会2014
    • Place of Presentation
      筑波大学東京キャンパス(東京都文京区)
    • Year and Date
      2014-10-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ホヤ遺伝子の命名法について2014

    • Author(s)
      佐藤 ゆたか、笹倉 靖徳、西田 宏記、遠藤 俊徳
    • Organizer
      ホヤ研究会2014
    • Place of Presentation
      筑波大学東京キャンパス(東京都文京区)
    • Year and Date
      2014-10-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脊索動物ホヤの変態開始を制御する機構2014

    • Author(s)
      笹倉 靖徳
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Knockout of genes in the chordate Ciona intestinalis2014

    • Author(s)
      Nicholas Treen, Haruka Sasaki, Keita Yoshida, Akiko Hozumi, Tetsushi Sakuma, Takashi Yamamoto, Yasunori Sasakura
    • Organizer
      日本発生生物学会年会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2014-05-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genome editing with TALENs and Crispr/Cas9 in the ascidian Ciona intestinalis.2014

    • Author(s)
      Nicholas Treen, Keita Yoshida, Haruka Sasaki, Tetsushi Sakuma, Narudo Kawai, Takashi Yamamoto, Yasunori Sasakura
    • Organizer
      International symposium on RNAi and genome editing methods
    • Place of Presentation
      徳島大学(徳島県徳島市)
    • Year and Date
      2014-03-14 – 2014-03-16
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] カタユウレイボヤにおけるCRISPR/Cas システムを用いたゲノム編集2013

    • Author(s)
      佐々木 陽香、吉田 慶太、笹倉 靖徳
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2013-12-05
    • Related Report
      2013 Annual Research Report
  • [Presentation] ホヤの変態を制御する分子基盤の解明2013

    • Author(s)
      笹倉靖徳
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2013-12-04
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工ヌクレアーゼTALEN を用いたカタユウレイボヤの突然変異体系統の作製2013

    • Author(s)
      吉田 慶太、Treen Nicholas、保住 暁子、佐久間 哲史、山本 卓、笹倉 靖徳
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2013-12-04
    • Related Report
      2013 Annual Research Report
  • [Presentation] カタユウレイボヤ卵を用いた母性mRNA の新規発現抑制系の開発2013

    • Author(s)
      飯塚 貴子、佐藤 瑛生、三田 薫、保住 暁子、濱田 麻友子、佐藤 矩行、笹倉 靖徳
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2013-12-04
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ascidian Genome Editing Using TALENs2013

    • Author(s)
      Nicholas Treen, Keita Yoshida, Tetsushi Sakuma, Haruka Sasaki, Narudo Kawai, Takashi Yamamoto and Yasunori Sasakura
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2013-12-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] 尾索動物のセルロース合成酵素の示す組織特異的発現の仕組み2013

    • Author(s)
      笹倉 靖徳、小椋 陽介、横森 類、朴 聖俊、中井 謙太、藤原 滋樹、吉田 慶太
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学(岡山市北区)
    • Year and Date
      2013-09-27
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脊索動物ホヤにおける遺伝学的技術を用いた発生メカニズムの解明2013

    • Author(s)
      笹倉 靖徳
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学(岡山市北区)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ホヤ幼生の変態における尾部吸収開始時間及び尾部吸収開始に必要な固着時間の定量的解析2013

    • Author(s)
      松延 祥平、笹倉 靖徳
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山大学(岡山市北区)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Annual Research Report
  • [Presentation] Exploring Functions Of Ascidian Hox Genes In Adult Tissue Formation2013

    • Author(s)
      Narudo Kawai, Keita Yoshida, Nicholas Treen, Haruka Sasaki, Tetsushi Sakuma, Tetsuro Ikuta, Mayuko Hamada, Hidetoshi Saiga, Takashi Yamamoto, Nori Satoh, Yasunori Sasakura
    • Organizer
      7th Tunicate Meeting
    • Place of Presentation
      Naples, Italy
    • Year and Date
      2013-07-25
    • Related Report
      2013 Annual Research Report
  • [Presentation] Disruption of Hox1 causes a homeotic transformation in the endostyle2013

    • Author(s)
      Keita Yoshida, Yosuke Ogura, Yasunori Sasakura
    • Organizer
      7th Tunicate Meeting
    • Place of Presentation
      Naples, Italy
    • Year and Date
      2013-07-23
    • Related Report
      2013 Annual Research Report
  • [Presentation] Coordination of mitosis and morphogenesis: Role of a prolonged G2 phase during chordate neural tube closure2013

    • Author(s)
      Yosuke Ogura, Asako Sakaue-Sawano, Masashi Nakagawa, Nori Satoh, Atsushi Miyawaki, Yasunori Sasakura
    • Organizer
      17th International Congress of Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      2013-06-16 – 2013-06-20
    • Related Report
      2013 Annual Research Report
  • [Presentation] Knockout of genes with TALE nucleases in the chordate Ciona intestinalis2013

    • Author(s)
      Yasunori Sasakura, Nicholas Treen, Haruka Sasaki, Narudo Kawai, Tetsushi Sakuma, Takashi Yamamoto, Keita Yoshida
    • Organizer
      The 46th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      くにびきメッセ(島根県松江市)
    • Year and Date
      2013-05-31
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] TALEN Gene Targeting: A New Frontier for Ascidian Genetics2013

    • Author(s)
      Nicholas Treen, Keita Yoshida, Narudo Kawai, Haruka Sasaki, Tetsushi Sakuma, Takashi Yamamoto, Yasunori Sasakura
    • Organizer
      The 46th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      くにびきメッセ(島根)
    • Year and Date
      2013-05-29 – 2013-05-31
    • Related Report
      2013 Annual Research Report
  • [Presentation] カタユウレイボヤ胚におけるZFNを用いたeGFP遺伝子への変異導入2011

    • Author(s)
      河合成道
    • Organizer
      第34回 日本分子生物学会年会
    • Place of Presentation
      横浜,・パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Sleeping Beautyトランスポゾンを用いた脊索動物カタユウレイボヤの形質転換2011

    • Author(s)
      保住暁子
    • Organizer
      第34回 日本分子生物学会年会
    • Place of Presentation
      横浜,・パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] ホヤのHox遺伝子群の機能解析とトランスジェニック系統について

    • Author(s)
      笹倉 靖徳
    • Organizer
      ホヤ研究会2012
    • Place of Presentation
      京都、京都大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 脊索動物ホヤにおけるセルロース合成酵素遺伝子の獲得とその機能

    • Author(s)
      笹倉 靖徳
    • Organizer
      日本進化学会第14回大会
    • Place of Presentation
      東京、首都大学東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 人工ヌクレアーゼを用いた脊索動物ホヤにおけるゲノム編集

    • Author(s)
      笹倉 靖徳
    • Organizer
      第35回 日本分子生物学会年会
    • Place of Presentation
      福岡、福岡国際会議場
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Germline transformation in the ascidian Ciona intestinalis

    • Author(s)
      笹倉 靖徳
    • Organizer
      2nd international symposium of the mechanisms of sexual reproduction in animals and plants
    • Place of Presentation
      名古屋、名古屋ガーデンパレス
    • Related Report
      2012 Annual Research Report
  • [Presentation] The resource project of Ciona intestinalis in Japan

    • Author(s)
      笹倉 靖徳
    • Organizer
      International Workshop on Tunicate Information System
    • Place of Presentation
      京都、京都大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Construction and application of TALENs in Ciona intestinalis

    • Author(s)
      Nicholas Treen
    • Organizer
      2012 Asia-Pacific Developmental Biology Conference
    • Place of Presentation
      Taiwan, Taipei
    • Related Report
      2012 Annual Research Report
  • [Presentation] Emerging technologies to investigate ascidian development

    • Author(s)
      Nicholas Treen
    • Organizer
      2013 Riken CDB symposium
    • Place of Presentation
      神戸、理化学研究所
    • Related Report
      2012 Annual Research Report
  • [Book] Germline transformation in the ascidian Ciona intestinalis.2014

    • Author(s)
      Yasunori Sasakura
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] ホヤにおけるTALENを用いた遺伝子改変//実験医学別冊 今すぐ始めるゲノム編集(山本卓編)2014

    • Author(s)
      Nicholas Treen, 吉田慶太、佐々木陽香、笹倉靖徳
    • Publisher
      羊土社
    • Related Report
      2013 Annual Research Report
  • [Book] 細胞工学// 脊索動物ホヤにおけるゲノム改変技術2013

    • Author(s)
      河合成道、Nicholas Treen、佐々木陽香、笹倉靖徳
    • Publisher
      秀潤社
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.shimoda.tsukuba.ac.jp/~sasakura/index.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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