Single-molecule observation of a higher order structure of a proton channel in influenza virus
| Project/Area Number |
23685002
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2011: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Keywords | タンパク質 / １分子観察 / 低温 / 分光 / 構造 / 物理化学 / １分子分光 / インフルエンザ / 単一分子分光 / 分子分光 / 一分子計測 / プロトンチャネル / 光学顕微鏡 / 実験 |
|---|
| Research Abstract |
A laser-scanning confocal microscope was developed for three-dimensional (3D) imaging and co-localization of single biomolecules immobilized at a temperature of 1.5 K. The performance was demonstrated by taking 3-color 3D emission image of single quantum dots and 3D co-localization of single dots. The 3D image size of single dot with excitation wavelength of 635, 532, and 405 nm were all within 1.2 times of the diffraction-limited size. The 3D co-localization precision of single dot in images taken with 635 and 532 nm excitation was 3 nm in the lateral (x, y) and 18 nm in the axial (z) directions.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Single-protein study of photoresistance of pigment-protein complex in lipid bilayer2011
Author(s)
A D.Uchiyama, H.Hoshino, K.Otomo, T.Kato, K.Onda, A.Watanabe, H.Oikawa, S.Fujiyoshi, M.Matsushita,_M.Nango, N.Watanabe, A.Sumino,_T.Dewa
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Journal Title
Chem.Phys.Lett.
Volume: 511
Issue: 1-3
Pages: 135-137
DOI
Related Report
Peer Reviewed
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