| Budget Amount *help |
¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2011: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Research Abstract |
A laser-scanning confocal microscope was developed for three-dimensional (3D) imaging and co-localization of single biomolecules immobilized at a temperature of 1.5 K. The performance was demonstrated by taking 3-color 3D emission image of single quantum dots and 3D co-localization of single dots. The 3D image size of single dot with excitation wavelength of 635, 532, and 405 nm were all within 1.2 times of the diffraction-limited size. The 3D co-localization precision of single dot in images taken with 635 and 532 nm excitation was 3 nm in the lateral (x, y) and 18 nm in the axial (z) directions.
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