Development of vacuum ultraviolet circular dichroism measurement system with reduction optics for conformational analysis of biomolecules
| Project/Area Number |
23685007
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
TANAKA Masahito 独立行政法人産業技術総合研究所, 計測フロンティア研究部門, 主任研究員 (30386643)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2011: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Keywords | 糖・糖鎖 / タンパク質 / 分子構造解析 / 真空紫外線 / 物性実験 / キラリティ / 化学物理 / バイオ関連機器 |
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| Research Abstract |
Circular dichroism spectrum measurement in the vacuum ultraviolet region has been widely applied to analyze the molecular conformation of chiral molecules. In this work, I have developed the circular dichroism measurement system in the vacuum ultraviolet region equipped with the reduction optics for conformational analysis of biomolecules such as protein and sugars. By using Schwarzschild mirror system as a reduction optic, the spot size of detection light was reduced to be about 0.04x0.08 mm at the focal point. The distortion of CD spectra was confirmed to be negligible. This system enables us to measure the CD spectra in the wavelength region from 140 to 350 nm. We succeeded in observing the circular dichroism spectra of some proteins and sugars in the vacuum ultraviolet region with the developing system.
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Report
(4 results)
Research Products
(32 results)
