Development of biosensing technologies in an optical pickup style

• Project/Area Number: 23685016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Osaka University
• Principal Investigator: YOSHIKAWA Hiroyuki 大阪大学, 工学(系)研究科(研究院), 助教 (00314378)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000); Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: バイオセンサー / 集光レーザー / 酵素 / 光捕捉 / 銀ナノ粒子 / 酸化重合 / 表面増強ラマン散乱 / グルコース / レーザー / 後方散乱光 / 酸化酵素

Research Abstract

An innovative biosensing technique working by simply focusing a single laser beam and detecting its reflection intensity in a manner similar to the optical pickup unit has been studied. Polyphenylenediamine nanostructures were deposited by a focused visible laser beam on a glass- o-phenylenediamine solution interface. Growth of the polymer nanostructure was monitored by a backscattered light intensity. Because the growth speed depends on light absorption by the oxidized o-PD, the backreflection oscillation curve can be used to monitor the peroxidase enzyme reaction in the solution. A reliable optical quantification of glucose can be performed in a short time. SERS based on molecular detections by using optical trapping of silver nanoparticles were also studied. Aggregates of silver nanoparticles and analyte molecules were produced by optical trapping and fixed on a glass substrate and the SERS spectra were measured by irradiating a visible laser beam on the aggregates.

Research Products

• [Journal Article] Double-Side-Coated Nanomechanical Membrane-Type Surface Stress Sensor (MSS) for One-Chip-One- Channel Setup (2013)
  • Author(s): Genki Yoshikawa, Frederic Loizeau, Cory J. Y. Lee, Terunobu Akiyama, Kota Shiba, Sebastian Gautsch, Terunobu Nakayama, Peter Vettiger, Nico F. De Rooij, Masakazu Aono
  • Journal Title: Langmuir Volume: 29 Issue: 24 Pages: 7551-7556
  • DOI: 10.1021/la3046719
  • Peer Reviewed
• [Journal Article] Two Dimensional array of Piezoresistive Nanomechanical Membrance-Type Surface Stress Sensor(MSS)with Improved Sensitivity (2012)
  • Author(s): Genki Yoshikawa, Terunobu Akiyama, Frederic Loizeau, Kota Shiba, Sebastian Gautsch, Tomonobu Nakayama, Peter Vettiger, Nico F.de Pooij, Masakazu Aono
  • Journal Title: Sensors Volume: 12 Issue: 11 Pages: 15873-15887
  • DOI: 10.3390/s121115873
  • Peer Reviewed
• [Journal Article] Single-Beam Optical Biosensing Based on Enzyme-Linked Laser Nanopolymerization of o-Phenylenediamine (2012)
  • Author(s): Hiroyuki Yoshikawa, Shuhei Imura, Eiichi Tamiya
  • Journal Title: Analytical Chemistry Volume: 84 Issue: 22 Pages: 9811-9817
  • DOI: 10.1021/ac301951w
  • Peer Reviewed
• [Presentation] Nanomechanical Membrane-type Surface Stress Sensor (MSS) as a Practical Sensing Platform (2014)
  • Author(s): Genki Yoshikawa
  • Organizer: TNT Japan 2014, Trends in NanoTechnology
  • Place of Presentation: Japan, Tokyo
  • Year and Date: 2014-01-30
  • Invited
• [Presentation] SERS解析用金ナノ粒子固定化基板の作製と生細胞イメージング (2014)
  • Author(s): 石橋達也，吉川裕之，民谷栄一
  • Organizer: 第61回応用物理学会春季学術講演会
  • Place of Presentation: 青山学院大学相模原キャンパス、神奈川県
• [Presentation] ペルオキシダーゼとナノ光重合を利用した抗原抗体反応のレーザー検出 (2014)
  • Author(s): 井村修平，吉川裕之，民谷栄一
  • Organizer: 第61回応用物理学会春季学術講演会
  • Place of Presentation: 青山学院大学相模原キャンパス、神奈川県
• [Presentation] 光トラップによる銀ナノ粒子凝集体の作製と生体分子のSERS解析 (2014)
  • Author(s): 尹航、吉川裕之、民谷栄一
  • Organizer: 第61回応用物理学会春季学術講演会
  • Place of Presentation: 青山学院大学相模原キャンパス、神奈川県
• [Presentation] Development of highly sensitive nanomechanical membrane-type surface stress sensor (MSS) (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: Award Ceremony of Tsukuba Encouragement Prize
  • Place of Presentation: Japan, Tsukuba
  • Year and Date: 2013-11-26
  • Invited
• [Presentation] Nanomechanical Sensors for Medical Applications in Gas and Liquid Phases (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: Swiss-Japanese Nanoscience Workshop "150 Years Diplomatic Relations Japan-Switzerland"
  • Place of Presentation: Japan, Tsukuba
  • Year and Date: 2013-10-09
  • Invited
• [Presentation] Possibilities and Challenges of Nanomechanical Sensors ～ towards Medical, Security, and Environmental Applications ～ (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: Seminar of JSAP Kansai branch
  • Place of Presentation: Japan, Suita
  • Year and Date: 2013-10-03
  • Invited
• [Presentation] Nanomechanical Membrane-type Surface Stress Sensor (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: Trends in NanoTechnology (TNT) 2013
  • Place of Presentation: Spain, Seville
  • Year and Date: 2013-09-13
  • Invited
• [Presentation] Nanomechanical Membrane-type Surface stress Sensor (MSS)～Application to in situ measurements of photo-polymerization～ (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: Annual Meeting on Photochemistry 2013
  • Place of Presentation: Japan, Matsuyama
  • Year and Date: 2013-09-11
  • Invited
• [Presentation] The necessities for the development of nanomechanical sensors : hand calculation and COMSOL (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: Seminar on microfluidics analysis
  • Place of Presentation: Japan, Tokyo
  • Year and Date: 2013-06-20
  • Invited
• [Presentation] Highly Sensitive Nanomechanical Sensor (MSS) (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: 15th Workshop of The Complex Electronics Function of Molecules for "Bio-compatible Electronics" (No. 181 Committee)
  • Place of Presentation: Japan, Tokyo
  • Year and Date: 2013-03-12
  • Invited
• [Presentation] Comprehensive Development of Nanomechanical Sensors ; MSS (2013)
  • Author(s): Genki Yoshikawa
  • Organizer: International Workshop on Micro- Probes and Microanalysis
  • Place of Presentation: China, Sanya
  • Year and Date: 2013-02-02
  • Invited
• [Presentation] Enzymatic Biosensing based on Laser Nanopolymerization of o-Phenylenediamine (2013)
  • Author(s): Hiroyuki YOSHIKAWA, Shuhei IMURA, Eiichi TAMIYA
  • Organizer: International Conference on Photochemistry (ICP2013)
  • Place of Presentation: ﾙｰｳﾞｧﾝｶﾄﾘｯｸ大学、ベルギー
• [Presentation] Single-beam optical biosensing using laser nanopolymerization and enzyme reactions (2013)
  • Author(s): Hiroyuki YOSHIKAWA, Shuhei IMURA, Eiichi TAMIYA
  • Organizer: SPIE2013, Optics + Photonics
  • Place of Presentation: San Diego Convention Center、アメリカ
• [Presentation] 全反射型LSPRバイオセンサ-によるイムノセンシングの特性評価 (2013)
  • Author(s): 吉川 裕之・井村 修平・民谷 栄一
  • Organizer: 第74回応用物理学会秋季学術講演会
  • Place of Presentation: 同志社大学京田辺キャンパス、京都府
• [Presentation] 集光レーザー場を利用した新規ナノバイオフォトニクス計測技術の開発 (2013)
  • Author(s): 吉川 裕之・井村 修平・尹 航・民谷栄一
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学東山キャンパス、愛知県
• [Presentation] 金ナノ粒子固定化基盤表面におけるマウス初代心筋細胞の分光イメージング (2013)
  • Author(s): 石橋 達也・吉川 裕之・池内 智彦・民谷 栄一
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学東山キャンパス、愛知県
• [Presentation] ペルオキシダーゼ酵素反応により促進されるナノ光重合反応を利用した迅速・高感度バイオセンシング (2013)
  • Author(s): 井村 修平・吉川 裕之・民谷 栄一
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学東山キャンパス、愛知県
• [Presentation] 光トラップ銀ナノ粒子を用いた表面増強ラマン散乱分光による局所バイオ分析 (2013)
  • Author(s): 尹 航・吉川 裕之・民谷 栄一
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学東山キャンパス、愛知県
• [Presentation] レーザー重合と酵素反応を利用したシングルビームバイオセンシング (2013)
  • Author(s): 吉川 裕之・井村 修平・民谷 栄一
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学、滋賀県
• [Presentation] 銀ナノ粒子の光捕捉を利用した表面増強ラマン散乱分光のバイオセンシング応用 (2013)
  • Author(s): 尹航，吉川裕之，民谷栄一
  • Organizer: 第60回応用物理学会春季学術講演会
  • Place of Presentation: 神奈川工科大学、神奈川県
• [Presentation] 集光レーザービームによるナノ光重合を利用した酸化酵素反応の迅速・高感度検出 (2013)
  • Author(s): 井村修平，吉川裕之，民谷栄一
  • Organizer: 第60回応用物理学会春季学術講演会
  • Place of Presentation: 神奈川工科大学、神奈川県
• [Presentation] 高感度リアルタイム測定を目指した全反射型LSPRバイオセンサ-の開発 (2013)
  • Author(s): 吉川 裕之，山本 英貴，民谷 栄一
  • Organizer: 第60回応用物理学会春季学術講演会
  • Place of Presentation: 神奈川工科大学、神奈川県
• [Presentation] Sensor Revolution - Possibilities of Nanomechanical Sensors (MSS) - (2012)
  • Author(s): Genki Yoshikawa
  • Organizer: MANA 5th Anniversary Memorial Symposium
  • Place of Presentation: Japan, Tsukuba
  • Year and Date: 2012-10-03
  • Invited
• [Presentation] Highly Sensitive Nanomechanical Membrane-type Surface Stress Sensor (MSS) (2012)
  • Author(s): Genki Yoshikawa
  • Organizer: The Current State and Future Perspectives of Organic Sensors
  • Place of Presentation: Japan, Wako
  • Year and Date: 2012-08-03
  • Invited
• [Presentation] 集光レーザーと酵素反応によるポリアニリンナノ構造の形成 (2012)
  • Author(s): 吉川裕之・内垣健・広中孝行・民谷栄一
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス、神奈川県
  • Year and Date: 2012-03-25
• [Presentation] バイオセンシングのためのレーザー誘起銀ナノ構造体形成 (2012)
  • Author(s): 吉川裕之・広中孝行・斉藤真人・民谷栄一
  • Organizer: 第59回応用物理学関係連合講演会
  • Place of Presentation: 早稲田大学、東京都
  • Year and Date: 2012-03-16
• [Presentation] 斜入射光に対する金ナノ粒子局在プラズモンバイオセンサー基板の反射スペクトル解析 (2012)
  • Author(s): 山本英貴,吉川裕之,民谷栄一
  • Organizer: 第59回応用物理学関係連合講演会
  • Place of Presentation: 早稲田大学、東京都
  • Year and Date: 2012-03-16
• [Presentation] Laser microfabrication of Plasmonic Silver Nanoparticles for Biosensing Applications (2012)
  • Author(s): 広中孝行・吉川裕之・斉藤真人・民谷栄一
  • Organizer: PITTCON 2012
  • Place of Presentation: Orange County Convention Center Orlando, FL USA
  • Year and Date: 2012-03-14
• [Presentation] Global challenges and possibilities of nanomechanical sensors ; MSS (2012)
  • Author(s): Genki Yoshikawa, Terunobu Akiyama, Sebastian Gautsch, Peter Vettiger
  • Organizer: MANA International Symposium 2012
  • Place of Presentation: Japan, Tsukuba
  • Year and Date: 2012-02-29
  • Invited
• [Presentation] Incident Angle Dependence of Reflection Spectrum of Gold Nanoparticle Plasmonic Sensors (2012)
  • Author(s): 山本英貴・吉川裕之・民谷栄一
  • Organizer: International Symposium on NanoPhotonics 2012
  • Place of Presentation: Friendship Hotel, Beijing, China
  • Year and Date: 2012-02-13
• [Presentation] Plasmonic Silver Nanoparticles Deposited by a Focused Laser Beam for Biosensing (2012)
  • Author(s): 広中孝行・吉川裕之・斉藤真人・民谷栄一
  • Organizer: 2012 Taiwan-Japan Nanophotonics and Plasmonic Metamaterials Workshop
  • Place of Presentation: National Taiwan University, Taipei, Taiwan
  • Year and Date: 2012-01-11
• [Presentation] 集光レーザー場がつくる分子・ナノ粒子集合構造の分光解析とバイオ計測への展開 (2012)
  • Author(s): 吉川裕之
  • Organizer: 第8回光科学若手研究会
  • Place of Presentation: 関西学院大学、兵庫県
• [Presentation] 全反射型ＬＳＰＲバイオセンサーの開発 (2012)
  • Author(s): 山本英貴，吉川裕之，民谷栄一
  • Organizer: 第6回バイオ関連化学シンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] 可視レーザー集光ビームによるポリフェニレンジアミンナノ構造体の形成と酵素反応検出 (2012)
  • Author(s): 吉川裕之・井村修平・民谷栄一
  • Organizer: 2012年光化学討論会
  • Place of Presentation: 東京工業大学、東京都
• [Presentation] Fabrication of Nano-Pillar Arrays for Plasmonic Biochip by Using Nanoimprint Technology with Porous Alumina Mold (2012)
  • Author(s): S. Jiang, M. Saito, H. Yoshikawa et al.
  • Organizer: 7th Photonics Center Symposium “Nanophotonics in Asia 2012”
  • Place of Presentation: 金沢エクセルホテル東急、石川県
• [Presentation] Surface enhanced Raman scattering study using gold nanoparticles for non-invasive assay of embryonic stem cell (2012)
  • Author(s): Hiroyuki Yoshikawa, Sathuluri R Rao, Eiichi Tamiya
  • Organizer: International Joint Symposium on Single-Cell Analysis
  • Place of Presentation: 京都リサーチパーク、京都府
• [Presentation] Highly sensitive membrane-type surface stress sensor (MSS) and its applications (2011)
  • Author(s): Genki Yoshikawa, Terunobu Akiyama, Sebastian Gautsch, Peter Vettiger, Heinrich Rohrer
  • Organizer: International Conference on Advanced Materials and Nanotechnology (ICAMN)
  • Place of Presentation: Nepal, Kathmandu
  • Year and Date: 2011-10-22
  • Invited
• [Presentation] OPTICAL BIOSENSING BY A LASER DEPOSITION OF SILVER NAN OPARTICLES (2011)
  • Author(s): 広中孝行・吉川裕之・斉藤真人・民谷栄一
  • Organizer: Sixth Photonics Center Symposium "Nanophotonics in Asia 2011"
  • Place of Presentation: 志摩観光ホテルクラシック、三重県
  • Year and Date: 2011-09-20
• [Presentation] レーザー誘起銀ナノ構造形成を利用した生体SERS測定用マイクロプローブの開発 (2011)
  • Author(s): 広中孝行・吉川裕之・斉藤真人・民谷栄一
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場、茨城県
  • Year and Date: 2011-09-12
• [Presentation] 斜入射光学系を用いた金ナノ粒子LSPRバイオセンサーの高感度化 (2011)
  • Author(s): 山本英貴・吉川裕之・民谷栄一
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場、茨城県
  • Year and Date: 2011-09-12
• [Presentation] Bioanalysis Using Nanoplasmonic Structures (2011)
  • Author(s): 吉川裕之
  • Organizer: The 14th Asian Chemical Congress
  • Place of Presentation: Queen Sirikit Convention Center, Bangkok,タイ(招待講演)
  • Year and Date: 2011-09-07
• [Presentation] "Nanomechanical Biosensors," Japan-Taiwan Joint Workshop on Nano Biomedical Engineering and Biosensing (2011)
  • Author(s): Genki Yoshikawa
  • Organizer: 20110727
  • Place of Presentation: Taiwan, Taichung
  • Year and Date: 2011-07-27
  • Invited
• [Book] NANOBIOSENSORS AND NANOBIOANALYSES, Chapter 9, Novel nanobiosensing using a focused laser beam (2014)
  • Author(s): Hiroyuki YOSHIKAWA
  • Total Pages: 300
  • Publisher: Springer
• [Book] "Nanomechanical Sensors and Membrane-type Surface Stress Sensor (MSS) for Medical, Security and Environmental Applications," in Manipulation of Nanoscale Materials : An Introduction to Nanoarchitectonics, edited by K. Ariga (2012)
  • Author(s): Genki Yoshikawa
  • Publisher: RSC Publishing, UK
• [Book] Nanomechanical Membrane-type Surface Stress Sensor (MSS) - for Medical, Security, and Environmental Applications, in Expected Materials for the Future (2012)
  • Author(s): Genki Yoshikawa
  • Publisher: NTS, Japan
• [Remarks] ホームページ
  • URL: http://y-genki.net/
• [Patent(Industrial Property Rights)] センサーチップホルダー (2013)
  • Inventor(s): 吉川元起、柴弘太
  • Industrial Property Rights Holder: 独立行政法人 物質・材料研究機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-096690
  • Filing Date: 2013-05-02
  • Overseas
• [Patent(Industrial Property Rights)] 被験物質の濃度測定方法および検出装置 (2013)
  • Inventor(s): 吉川 裕之・井村 修平・民谷 栄一
  • Industrial Property Rights Holder: 吉川 裕之・井村 修平・民谷 栄一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-218750
  • Filing Date: 2013-10-21
• [Patent(Industrial Property Rights)] 両面被覆表面応力センサー (2012)
  • Inventor(s): 吉川元起、青野正和、中山知信、フレ デリックロワゾ、秋山照伸、セバスチャンガ ウチ、ピーターフェッティガー
  • Industrial Property Rights Holder: 独立行政法人 物質・材料研究機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-094299
  • Filing Date: 2012-04-17
  • Overseas