Development of biosensing technologies in an optical pickup style
| Project/Area Number |
23685016
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Keywords | バイオセンサー / 集光レーザー / 酵素 / 光捕捉 / 銀ナノ粒子 / 酸化重合 / 表面増強ラマン散乱 / グルコース / レーザー / 後方散乱光 / 酸化酵素 |
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| Research Abstract |
An innovative biosensing technique working by simply focusing a single laser beam and detecting its reflection intensity in a manner similar to the optical pickup unit has been studied. Polyphenylenediamine nanostructures were deposited by a focused visible laser beam on a glass- o-phenylenediamine solution interface. Growth of the polymer nanostructure was monitored by a backscattered light intensity. Because the growth speed depends on light absorption by the oxidized o-PD, the backreflection oscillation curve can be used to monitor the peroxidase enzyme reaction in the solution. A reliable optical quantification of glucose can be performed in a short time. SERS based on molecular detections by using optical trapping of silver nanoparticles were also studied. Aggregates of silver nanoparticles and analyte molecules were produced by optical trapping and fixed on a glass substrate and the SERS spectra were measured by irradiating a visible laser beam on the aggregates.
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Report
(4 results)
Research Products
(53 results)
