| Budget Amount *help |
¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
Fluorogenic substrates or probes for chemical transformations, molecules that show no or very low fluorescence but show high fluorescence upon chemical transformations, are useful for monitoring the progress of chemical transformations, especially at the initial stages of the reaction. Assays using fluorogenic substrates accelerate the identification of superior catalysts and reaction conditions in high-throughput formats as well as characterization of catalysis on a small scale. Here, we studied the development of aldehyde, imine, and enals-type fluorogenic probes and their use in monitoring various organocatalytic chemical transformations, including C-C bond-forming reactions such as aldol, Mannich, and Diels-Alder reactions. The best combinations of amine catalyst and acid additive in both enamine and iminium catalysis were identified. In conclusion, we have developed fluorogenic compounds that can be used for rapid monitoring chemical reactions through increased fluorescence.
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