Development of artificial organelle through the controlled cellular uptake of polyion complex vesicles
Project/Area Number |
23685037
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Chemistry related to living body
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Research Institution | Kyushu University (2013) The University of Tokyo (2011-2012) |
Principal Investigator |
KISHIMURA Akihiro 九州大学, 工学(系)研究科(研究院), 准教授 (70422326)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
Fiscal Year 2013: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2011: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
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Keywords | ポリイオンコンプレックス / ベシクル / ブロック共重合体 / 人工オルガネラ / ドラッグデリバリーシステム / 酵素 / ナノリアクター / 人口オルガネラ / タンパク質デリバリー / 自己組織化 / バイオマテリアル |
Research Abstract |
We have so far developed polyion complex vesicles (PICsomes) with well-controlled size in the size ranging from 100 to 400 nm. PICsomes are characterized by their biocompatibility and semipermeable vesicle membrane. Therefore, enzyme-loaded PICsomes are promising for enzyme-based nanoreactors, which can work inside of cells as an artificial organelle. In this research project, we tried to develop "artificial organelle"utilizing PICsomes. The preparation method of nano-reactors was successfully established through encapsulation of enzymes in PICsomes without deactivation. Also, we investigated the interaction between chemically-modified PICsomes and cells, and evaluated and found major factors for further cellular uptake. Results indicated that degree of cross-linking of PIC membrane and deformability of PICsomes contribute cellular uptake to some extent.
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Report
(4 results)
Research Products
(130 results)
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[Journal Article] Bioactivepolymeric metallosomesself-assembled through blockcopolymer-metal complexation.2012
Author(s)
K. Osada, H. Cabral, Y. Mochida, S. -ELee, K. Nagata, T. Matsuura, M.Yamamoto, Y. Anraku, A. Kishimura, N.Nishiyama, K. Kataoka
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Journal Title
J. Am.Chem. Soc.
Volume: 134(32)
Issue: 32
Pages: 13172-13175
DOI
Related Report
Peer Reviewed
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[Journal Article] NanoPARCEL : a method for controlling cellular behavior with external light.2012
Author(s)
Murayama, M., Su, B., Okabe, K., Kishimura, A., Osada, K., Miura, M., Funatsu, T., Kataoka, K., and Kato, M
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Journal Title
Chem Comm.
Volume: 48
Issue: 67
Pages: 8380-8382
DOI
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Peer Reviewed
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[Presentation] Development of enzyme-loaded PICsomes for enzyme/prodrug therapy
Author(s)
Y. Anraku, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, A. Kishimura, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
Organizer
40th Annual Meeting & Exposition of the Controlled Release Society
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii, USA.
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[Presentation] Development of enzyme-loaded nano- sized polyion complex vesicles (PICsomes) for enzyme/prodrug therapy
Author(s)
Y. Anraku, A. Kishimura, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
Organizer
第35回日本バイオマテリアル学会大会
Place of Presentation
タワーホール船堀、東京
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[Book] 有機機能材料基礎から応用まで2014
Author(s)
松浦和則, 角五彰, 岸村顕広, 佐伯昭紀, 竹岡敬和, 内藤昌信, 中西尚志, 舟橋正浩, 矢貝史樹
Publisher
講談社 (分担執筆;第10章、第12章担当), (ISBN978-4-06-156802-0)
Related Report
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[Book] 有機機能材料 基礎から応用まで2014
Author(s)
松浦和則, 角五 彰, 岸村顕広, 佐伯昭紀, 竹岡敬和, 内藤昌信, 中西尚志, 舟橋正浩, 矢貝史樹
Total Pages
256
Publisher
講談社
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