| Budget Amount *help |
¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2011: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
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| Research Abstract |
In order to elucidate intracellular signal transduction pathway at the molecular level, it is necessary to detect the temporal and spatial changes of intracellular signaling molecules. For this purpose, we have developed several metal ion fluorescent probes that can specifically localize cell organelle. Attachment of cholesterol or cationic functional groups to the probe skeletons makes it possible to accumulate the probes in the appropriate sites in the cell. By using these probes, I have succeeded in monitoring the local concentration changes of Zn(II) and Cu(I) in fluorescence.
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