Real-time observation of proton-pumping of membrane protein complexes using a novel high-sensitivity IR spectrometer
Project/Area Number |
23685040
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Chemistry related to living body
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Research Institution | Institute of Physical and Chemical Research (2012-2013) University of Hyogo (2011) |
Principal Investigator |
KUBO Minoru 独立行政法人理化学研究所, 放射光科学総合研究センター, 研究員 (90392878)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
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Keywords | 赤外分光法 / 時間分解振動分光法 / ヘムタンパク質 / 膜タンパク質 / チトクロム酸化酵素 / プロトンポンプ / 一酸化窒素還元酵素 / フェムト秒レーザー / 時間分解赤外分光 / 酵素反応 / 光反応 |
Research Abstract |
This project aims to establish time-resolved IR spectroscopic techniques for observing reactions of membrane protein complexes under physiological conditions in real time. High-sensitivity IR spectrometer was constructed using a femtosecond IR laser as the light source. On the other hand, a flow-flash measurement system was developed to control reactions, which are triggered by the photolysis of caged compounds. Micro-channel flow cell was used to obtain a spectrum with minimal sample consumption. The flow-flash system was installed into time-resolved UV-visible absorption spectrometer and tested on NO reductase. We successfully observed the enzymatic reaction of this membrane protein with microsecond time resolution. Flow-flash time-resolved IR spectroscopy is ongoing.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Copper(I)-dioxygen reactivity in a sterically demanding tripodal tetradentate tren ligand. Formation and reactivity of a mononuclear copper(II) end-on superoxo complex2012
Author(s)
Kobayashi, Y., Okubo, K., Nomura, T., Kubo, M., Fujieda, N., Sugimoto, H., Fukuzumi, S., Goto, K., Ogura, T., Itoh, S
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Journal Title
Eur. J. Inorg. Chem
Volume: (29) (Selected for Cover Picture)
Issue: 29
Pages: 4574-4578
DOI
Related Report
Peer Reviewed
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[Journal Article] Chromium(IV)-peroxo complex formation and its nitric oxide dioxygenase reactivity2012
Author(s)
Yokoyama, A., Han, J. E., Cho, J., Kubo, M., Ogura, T., Siegler, M. A., Karlin, K. D., Nam, W
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Journal Title
J. Am. Chem. Soc
Volume: Vol. 134
Issue: 37
Pages: 15269-15272
DOI
Related Report
Peer Reviewed
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[Journal Article] Chromium(V)-oxo and chromium(III)-superoxo complexes bearing a macrocyclic TMC ligand in hydrogen atom abstraction reactions2011
Author(s)
Cho, J., Woo, J., Han, J. E., Kubo, M., Ogura, T., Nam, W
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Journal Title
Chem. Sci
Volume: Vol. 2
Issue: 10
Pages: 2057-2062
DOI
Related Report
Peer Reviewed
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[Journal Article] Spectroscopic identification of reactive porphyrin motions2011
Author(s)
Barabanschikov, A., Demidov, A., Kubo, M., Champion, P.M., Sage, J.T., Zhao, J., Sturhahn, W., Alp, E.E.
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Journal Title
J.Chem.Phys.
Volume: 135
Issue: 1
DOI
Related Report
Peer Reviewed
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[Journal Article] Chromium(V)-oxo and chromium(III)-superoxo complexes bearing a macrocyclic TMC ligand in hydrogen atom abstraction reactions2011
Author(s)
Cho, J., Woo, J., Han, J.E., Kubo, M., Ogura, T., Nam, W.
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Journal Title
Chem.Sci.
Volume: 2
Pages: 2057-2062
Related Report
Peer Reviewed
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[Journal Article] Spectroscopic and computational characterization of Cu^<II>-OOR (R=H or Cumyl) complexes bearing a Me_6-tren ligand2011
Author(s)
Choi, Y.J., Cho, K.-B., Kubo, M., Ogura, T., Karlin, K.D., Cho, J., Nam, W.
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Journal Title
Dalton Trans.
Volume: 40
Issue: 10
Pages: 2234-2241
DOI
Related Report
Peer Reviewed
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[Presentation] Development of a high-sensitivity time-resolved infrared spectrometer and its application to cytochrome c oxidase in aqueous solution2013
Author(s)
Kubo, M., Nakashima, S., Yamaguchi, S., Ogura, T., Mochizuki, M., Kang, J., Tateno, M., Shinzawa-Itoh, K., Kato, K., Yoshikawa, S
Organizer
International Conference on Structural Genomics-Structural Life Science (ICSG-2013-SLS)
Place of Presentation
Sapporo (Japan)
Related Report
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[Presentation] Real time observation of water-channel closure controlled by Cu_B in bovine cytochrome c oxidase using a novel high-sensitivity infrared system2013
Author(s)
Kubo, M., Nakashima, S., Yamaguchi, S., Ogura, T., Mochizuki, M., Kang, J., Tateno, M., Shinzawa-Itoh, K., Kato, K., Yoshikawa, S
Organizer
16th International Conference on Biological Inorganic Chemistry (ICBIC-16)
Place of Presentation
Grenoble (France)
Related Report
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[Presentation] Development of a high-sensitivity time-resolved infrared spectrometer and its application to cytochrome c oxidase in aqueous solution2013
Author(s)
Kubo, M., Nakashima, S., Yamaguchi, S., Ogura, T., Mochizuki, M., Kang, J., Tateno, M., Shinzawa-Itoh, K., Kato, K., Yoshikawa, S.
Organizer
International Conference on Structural Genomics-Structural Life Science (ICSG-2013-SLS)
Place of Presentation
札幌
Related Report
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[Presentation] Real time observation of water-channel closure controlled by CuB in bovine cytochrome c oxidase using a novel high-sensitivity infrared system2013
Author(s)
Kubo, M., Nakashima, S., Yamaguchi, S., Ogura, T., Mochizuki, M., Kang, J., Tateno, M., Shinzawa-Itoh, K., Kato, K., Yoshikawa, S.
Organizer
16th International Conference on Biological Inorganic Chemistry (ICBIC-16)
Place of Presentation
Grenoble (France)
Related Report
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