Fundamental study on physiological properties of polymer-brush-afforded hybrid particles

• Project/Area Number: 23685049
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Polymer/Textile materials
• Research Institution: Kyoto University
• Principal Investigator: OHNO Kohji 京都大学, 化学研究所, 准教授 (00335217)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000); Fiscal Year 2013: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2011: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: リビングラジカル重合 / グラフト重合 / ポリマーブラシ / 微粒子 / 体内動態 / 高分子合成 / 高分子構造・物性 / 生体材料 / ナノ材料 / 表面・界面物性

Research Abstract

Perfectly dispersive polymer-brush-afforded hybrid particles were synthesized by surface-initiated living radical polymerization. The protein adsorption properties and circulation lifetime in the blood of the resultant hybrids particles were significantly dependent on their structural parameters. Some hybrid particles exhibiting an excellently prolonged circulation lifetime preferentially accumulated in tumor tissue. The tumor-targeted delivery was optically visualized using hybrid particles grafted with fluorescence-labeled polymer brushes. In addition, the tumor-targeted delivery of polymer-brush-afforded iron oxide nanoparticles was visualized by an MRI measurement.

Research Products

• [Journal Article] Fabrication of Contrast Agents for Magnetic Resonance Imaging from Polymer-Brush-Afforded Iron Oxide Magnetic Nanoparticles Prepared by Surface-Initiated Living Radical Polymerization (2013)
  • Author(s): Kohji Ohno, Chizuru Mori, Tatsuki Akashi, Shinichi Yoshida, Yoshiyuki Tago, Yoshinobu Tsujii, Yasuhiko Tabata
  • Journal Title: Biomacromolecules Volume: 14 Issue: 10 Pages: 3453-3462
  • DOI: 10.1021/bm400770n
  • Peer Reviewed
• [Journal Article] Monodisperse, Charge-Stabilized, Core-Shell Particles via Silica-Supported RAFT Polymerization for Cell Imaging (2013)
  • Author(s): John Moraes, Kohji Ohno, Thomas Maschmeyer, Sébastien Perrier
  • Journal Title: Chemistry of Materials Volume: 25 Issue: 17 Pages: 3522-3527
  • DOI: 10.1021/cm401957m
  • Peer Reviewed
• [Journal Article] Surface-initiated living radical polymerization from silica particles functionalized with poly(ethylene glycol)-carrying initiator (2013)
  • Author(s): Ohno, Kohji; Tabata, Hiroshi; Tsujii, Yoshinobu
  • Journal Title: Colloid and Polymer Science Volume: 291 Issue: 1 Pages: 127-135
  • DOI: 10.1007/s00396-012-2734-7
  • Peer Reviewed
• [Journal Article] Blood Clearance and Biodistribution of Polymer Brush-Afforded Silica Particles Prepared by Surface-Initiated Living Radical Polymerization (2012)
  • Author(s): Kohji Ohno, Tatsuki Akashi, Yoshinobu Tsujii, Masaya Yamamoto, Yasuhiko Tabata
  • Journal Title: Biomacromolecules Volume: 13 Issue: 3 Pages: 927-936
  • DOI: 10.1021/bm201855m
  • Peer Reviewed
• [Journal Article] Surface-Initiated Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization from Fine Particles Functionalized with Trithiocarbonates (2011)
  • Author(s): Kohji Ohno, Ying Ma, Yun Huang, Chizuru Mori, Yoshikazu Yahata, Yoshinobu Tsujii, Thomas Maschmeyer, John Moraes, Sébastien Perrier
  • Journal Title: Macromolecules Volume: 44 Issue: 22 Pages: 8944-8953
  • DOI: 10.1021/ma202105y
  • Peer Reviewed
• [Journal Article] Materials Designing by High-Pressure Living Radical Polymerization (2011)
  • Author(s): 大野工司
  • Journal Title: The Review of High Pressure Science and Technology Volume: 21 Issue: 1 Pages: 4-10
  • DOI: 10.4131/jshpreview.21.4
  • NAID: 10028049250
  • ISSN: 0917-639X, 1348-1940
  • Peer Reviewed
• [Presentation] Physiological Properties of Polymer Brush-Afforded Nanoparticles Prepared by Surface-Initiated Living Radical Polymerization (2013)
  • Author(s): K. Ohno, Y. Tsujii, Y. Tabata
  • Organizer: 87^<th> Colloid and Surface Science Symposium
  • Place of Presentation: USA
  • Year and Date: 2013-06-26
• [Presentation] Bioimaging with Polymer Brush-Decorated Magnetic Nanoparticles Synthesized by Surface-Initiated Living Radical Polymerization (2013)
  • Author(s): K. Ohno, C. Mori, S. Yoshida, Y. Tago
  • Organizer: The 11th International Conference on Ferrites
  • Place of Presentation: Naha
  • Year and Date: 2013-04-18
• [Presentation] Polymer Brush-Afforded Fine Particles Synthesized by Surface-Initiated Living Radical Polymerization (2011)
  • Author(s): K. Ohno
  • Organizer: 2011 ASEPFPM
  • Place of Presentation: Hong Kong
  • Year and Date: 2011-12-12
• [Presentation] Polymer Brush-Afforded Hybrid Particles Synthesized by Surface-Initiated Radical Polymerization (2011)
  • Author(s): 大野工司
  • Organizer: The 4th Asian Symposium on Emulsion Polymerization and Functional Polymeric Microspheres
  • Place of Presentation: The Hong Kong Polytechnic University, Hong Kong, China
  • Year and Date: 2011-12-12
• [Presentation] 表面開始リビングラジカル重合によるポリマーブラシ付与複合微粒子の精密合成とその応用 (2011)
  • Author(s): 大野工司
  • Organizer: 第81回千葉地域活動高分子研究交流講演会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-08
• [Presentation] 表向開始リビングラジカル重合によるポリマーブラシ付与複合微粒子の精密設計とその応用 (2011)
  • Author(s): 大野工司
  • Organizer: 第81回千葉地域活動高分子研究交流講演会
  • Place of Presentation: 千葉大学
  • Year and Date: 2011-11-08
• [Presentation] リビングラジカル重合によるポリマーブラシ/無機微粒子複合材料の精密設計 (2011)
  • Author(s): 大野工司
  • Organizer: 「ソフトインターフェースの分子科学」ワークショップ
  • Place of Presentation: パレブラン高志会館
  • Year and Date: 2011-11-04
• [Presentation] ポリマーブラシ付与複合微粒子の構造因子がタンパク質吸着量に及ばす影響に関する研究 (2011)
  • Author(s): 中川順一、大野工司、辻井敬亘
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-29
• [Presentation] リビングラジカル重合による材料設計 (2011)
  • Author(s): 大野工司
  • Organizer: 高分子界面科学の新展開
  • Place of Presentation: 富山大学
  • Year and Date: 2011-09-10
• [Presentation] ポリマーブラシ付与微粒子の精密設計と機能 (2011)
  • Author(s): 大野工司
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-05-26
• [Presentation] ポリマーブラシ付与微粒子の精密設計と機能 (2011)
  • Author(s): 大野工司
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-05-25
• [Presentation] 表面開始リビングラジカル重合により合原したポリマーブラシ付与複合微粒子予とタンパク質との相互作用 (2011)
  • Author(s): 中川順一、大野工司、辻井敬亘
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-05-25
• [Presentation] Physiological properties of polymer brush-decorated fine particles prepared by surface-initiated living radical polymerization
  • Author(s): 大野工司
  • Organizer: 14th International Association of Colloidal and Interface Scientists, Conference
  • Place of Presentation: 仙台国際センター（仙台市）
• [Presentation] 構造の明確なポリマーブラシ付与複合微粒子構造のタンパク質吸着特性
  • Author(s): 大野工司
  • Organizer: 平成 24年度繊維学会秋季研究発表会
  • Place of Presentation: 福井大学（福井市）
• [Presentation] 高分子鎖をブラシ状に付与した微粒子の精密設計と応用
  • Author(s): 大野工司
  • Organizer: イノベーションジャパン２０１２
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
• [Patent(Industrial Property Rights)] 複合粒子を含むMRI造影剤 (2011)
  • Inventor(s): 多胡 善幸、吉田 慎一、大野 工 司、辻井 敬亘、田畑 泰彦
  • Industrial Property Rights Holder: 多胡 善幸、吉田 慎一、大野 工 司、辻井 敬亘、田畑 泰彦
  • Filing Date: 2011-09-29
  • Overseas