| Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2013: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Research Abstract |
We investigated various viruses in water environment using next-generation sequencing (NGS) technology. Component technologies such as pre-treatment of environmental samples, molecular technologies for sequencing and bioinformatic analysis of obtained sequence data were examined and developed. In genotyping of noroviruses in wastewater, we could detect various genotypes including minor types such as GII.2. In metagenomic analysis of RNA viruses, we found that most viruses in wastewater are unidentified. We could detect viruses from 11 genera, some of which have not identified in Japan. These findings demonstrated that the developed methods for NGS analysis are very useful in identifying viruses in the environment.
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