| Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Outline of Final Research Achievements |
Here, we tried to develop the generation process of integrating the second specificity into a monoclonal antibody. Based on an anti-human epidermal growth factor receptor (EGFR) antibody, we examined a mutation site, mutant amino acid species, and evaluation method of binding activity. Using multiple libraries and living cell panning with mammalian cells artificially expressing target antigens, we finally succeeded in integrating another specificity into anti-EGFR antibody and evaluated them using enzyme-linked immunosorbent assay. We also confirmed general versatility of this process through developing another antibody with two specificities.
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