Lipoprotein lipidomics of oxystress-induced diseases by using supercritical fluid technologies
| Project/Area Number |
23686120
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
BAMBA Takeshi 大阪大学, 工学(系)研究科(研究院), 准教授 (10432444)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUSAKI Eiichiro 大阪大学, 大学院工学研究科, 教授 (40273594)
YOSHIDA Masaru 神戸大学, 大学院医学研究科, 准教授 (00419475)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | メタボロミクス / 脂質 / リポプロテイン / 超臨界流体 / SFC/MS / SFE / メタボローム / SFC / 質量分析 |
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| Research Abstract |
We successfully developed an analysis method for comprehensive lipoprotein lipid profiling. The constructed high-resolution and high-throughput system uses supercritical fluid chromatography/mass spectrometry (SFC/MS) and on-line supercritical fluid extraction system. To determine the applicability of this system to real samples, we performed lipidomics analyses on myocardial infarction-prone rabbits and high-risk myocardial infarction patients. Disease-specific profiles were obtained and marker metabolites were identified. Furthermore, using our developed method, we identified metabolic variations that could not be identified by using the conventional method, which targets serum lipids by performing analyses on lipoprotein fractions.
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Report
(4 results)
Research Products
(185 results)
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[Journal Article] Supercritical fluid chromatography/Orbitrap mass spectrometry based lipidomics platform coupled with automated lipid identification software for accurate lipid profiling2013
Author(s)
Yamada, T., Uchikata, T., Sakamoto, S., Yokoi, Y., Nishiumi, S., Yoshida, M., Fukusaki, E., Bamba, T
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Journal Title
J. Chromatogra. A
Volume: 1301
Pages: 237-242
DOI
Related Report
Peer Reviewed
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[Journal Article] Data Assessment and Metabolite Identification Tool for Large-scale MRM-based Widely Targeted Metabolomics2013
Author(s)
Tsugawa, H., Arita, M., Kanazawa, M., Ogiwara, A., Bamba, T., Fukusaki, E
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Journal Title
Anal. Chem
Volume: 85(10)
Issue: 10
Pages: 5191-5199
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of a lipid profiling system using reverse-phase liquid chromatography coupled to high-resolution mass spectrometry with rapid polarity switching and an automated lipid identification software2013
Author(s)
Yamada, T., Uchikata, T., Sakamoto, S., Yokoi, Y., Fukusaki, E., Bamba, T
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Journal Title
J. Chromatogra. A
Volume: 1292
Pages: 211-218
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of oxidized phosphatidylcholine isomer profiling method using supercritical fluid chromatography/tandem mass spectrometry2012
Author(s)
Uchikata, T., Matsubara, A., Nishiumi, S., Yoshida, M., Fukusaki, E., Bamba, T
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Journal Title
J. Chromatogra. A
Volume: 1250
Pages: 205-211
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of Biomarkers of Stent Restenosis With Serum Metabolomic Profiling Using Gas Chromatography/Mass Spectrometry2012
Author(s)
Hasokawa, M., Shinohara, M., Tsugawa, H., Bamba, T., Fukusaki, E., Nishiumi, S., Nishimura, K., Yoshida, M., Ishida, T., Hirata, K
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Journal Title
Circulation Journal
Volume: 76
Issue: 8
Pages: 1864-1873
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed
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[Journal Article] Development of a polar lipid profiling method by supercritical fluid chromatography/mass spectrometry. Development of a polar lipid profiling method by supercritical fluid chromatography/mass spectrometry2011
Author(s)
Lee, J.W., Yamamoto, T., Uchikata, T., Matsubara, A., Fukusaki, E., Bamba, T
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Journal Title
J. Sep. Sci
Volume: 34(24)
Issue: 24
Pages: 3553-3560
DOI
Related Report
Peer Reviewed
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[Journal Article] Ametabolomic approach to detect early stages of lung cancers2011
Author(s)
Hori, S., Nishiumi, S., Kobayashi, K., Shinohara, M., Hatakeyama, Y.,Kotani, Y., Hatano, N., Maniwa, Y., Nishio, W., Bamba, T., Fukusaki, E., Azuma, T., Takenawa, T., Nishimura, Y., Yoshida, M
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Journal Title
Lung Cancer
Volume: 74
Issue: 2
Pages: 284-292
DOI
Related Report
Peer Reviewed
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[Journal Article] Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase B and regulates mitochondrial complex V activity
Author(s)
Rahman, M., Nirala N.K., Singh, A., Zhu, L.J., Taguchi, K., Fukusaki, E., Bamba, T., Shaw, L.M. Lambright, D.G. Acharya, J.K. Acharya, U.R. Drosophila
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Journal Title
J. Cell Biol
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Identification of biomarkers of stent restenosis with serum metabolomic profiling using gas chromatography/mass spectrometry
Author(s)
Hasokawa, M., Shinohara, M., Tsugawa, H., Bamba, T., Fukusaki, E., Nishiumi, S., Nishimura, K., Yoshida, M., Ishida, T., Hirata, K.
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Journal Title
Circulation Journal
Volume: (in press)(印刷中)
NAID
Related Report
Peer Reviewed
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