Development of bio-actuator using tissue-engineered skeletal muscle

• Project/Area Number: 23686121
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyushu University
• Principal Investigator: ITO Akira 九州大学, 工学(系)研究科(研究院), 准教授 (60345915)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000); Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 骨格筋 / 再生医療 / ティッシュエンジニアリング / アクチュエータ / 人工筋組織 / 遺伝子導入 / 組織培養 / 三次元組織 / バイオアクチュエータ / 筋芽細胞 / 筋組織 / 磁性ナノ粒子 / リポソーム / マグネタイト

Research Abstract

The development of new actuators which can mimic living muscle performances would open new horizons in robotics field. We applied a magnetic force-based tissue engineering (Mag-TE) technique to skeletal muscle tissue engineering, and succeeded to construct highly cell-dense, aligned and contractile skeletal muscle-like tissues by using a genetic engineering approach and electrical pulse stimulation culture.

Research Products

• [Journal Article] Effects of B-Cell Lymphoma 2 Gene Transfer to Myoblast Cells on Skeletal Muscle Tissue Formation Using Magnetic Force-Based Tissue Engineering (2013)
  • Author(s): Sato, et al
  • Journal Title: Tissue Engineering Part A Volume: 19 Issue: 1-2 Pages: 307-315
  • DOI: 10.1089/ten.tea.2011.0728
  • Peer Reviewed
• [Journal Article] Hollow Fiber Bioreactor Perfusion Culture System for Magnetic Force-Based Skeletal Muscle Tissue Engineering (2012)
  • Author(s): Yamamoto, et al
  • Journal Title: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN Volume: 45 Issue: 5 Pages: 348-354
  • DOI: 10.1252/jcej.11we237
  • NAID: 130001858819
  • ISSN: 0021-9592, 1881-1299
  • Peer Reviewed
• [Presentation] 熱ストレスとビタミンC添加による人工骨格筋組織の機能強化 (2014)
  • Author(s): 池田一史、佐藤暢哲、菅野翔太、井藤彰、河邉佳典、上平正道
  • Organizer: 化学工学会
  • Place of Presentation: 岐阜県岐阜市
• [Presentation] Medical Application of Magnetite Nanoparticles (2014)
  • Author(s): Akira Ito
  • Organizer: 化学工学会
  • Place of Presentation: 岐阜県岐阜市
• [Presentation] 人工筋組織における熱ストレスとビタミンC添加の筋機能への影響 (2013)
  • Author(s): 佐藤暢哲、池田一史、菅野翔太、井藤彰、河邉佳典、上平正道
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島市
• [Presentation] Magnetic Force-Based Tissue Engineering for Construction of Functional Skeletal Muscle Tissues (2013)
  • Author(s): Masanori Sato, Akira Ito, Shota Kanno, Yoshinori Kawabe
  • Organizer: World Biotechnology Congress 2013
  • Place of Presentation: Boston, USA
• [Presentation] 組織工学的手法で作製した骨格筋の電気刺激培養による機能強化 (2013)
  • Author(s): 井藤彰、山本泰徳、藤田英明、長森英二、河邉佳典、上平正道
  • Organizer: 化学工学会
  • Place of Presentation: 大阪市
• [Presentation] Magnetic Force-based tissue engineering for construction of functional skeletal muscle tissues (2013)
  • Author(s): Masanori Sato, Akira Ito, Shota Kanno, Yoshinori Kawabe, Masamichi Kamihira
  • Organizer: World Biotechnology Congress 2013
  • Place of Presentation: Boston, USA
• [Presentation] 組織工学的手法で作製した骨格筋の電気刺激培養による機能強化 (2013)
  • Author(s): 井藤 彰 ら
  • Organizer: 化学工学会第78年会
  • Place of Presentation: 大阪市
• [Presentation] バイオアクチュエータ開発を目指したIGF-I遺伝子導入筋芽細胞の挙動解析 (2012)
  • Author(s): 山本昌弘、佐藤暢哲、山本泰徳、井藤彰、河邉佳典、上平正道
  • Organizer: 化学関連支部合同九州大会
  • Place of Presentation: 北九州市
  • Year and Date: 2012-06-30
• [Presentation] Construction of Skeletal Muscle Tissues Using Genetically Engineered Myoblast Cells (2012)
  • Author(s): Masanori Sato, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira
  • Organizer: JAACT 2012
  • Place of Presentation: 名古屋市
• [Presentation] 電気刺激による高機能人工筋組織の作製 (2012)
  • Author(s): 山本泰徳、井藤彰、河邉佳典、藤田英明、長森英二、上平正道
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸市
• [Presentation] Medical Application of Functional Magnetite Nanoparticles (2012)
  • Author(s): Akira Ito
  • Organizer: International Conference of the Asia Union of Magnetics Societies 2012
  • Place of Presentation: 奈良市
• [Presentation] Bcl-2遺伝子導入によるアポトーシス耐性筋組織の作製 (2012)
  • Author(s): 佐藤暢哲、井藤彰、秋山裕和、河邉佳典、上平正道
  • Organizer: 化学工学会
  • Place of Presentation: 東京都
• [Presentation] Medical application of functional magnetite nanoparticles. (2012)
  • Author(s): Akira Ito
  • Organizer: International Conference of the Asian Union of Magnetics Societies 2012
  • Place of Presentation: Nara, Japan
  • Invited
• [Presentation] 電気刺激による高機能人工筋組織の作製 (2012)
  • Author(s): 山本泰徳 ら
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸市
• [Presentation] Construction of skeletal muscle tissues using genetically engineered myoblast cells. (2012)
  • Author(s): Masanori Sato et al.
  • Organizer: JAACT2012
  • Place of Presentation: 名古屋市
• [Presentation] バイオアクチュエータ開発を目指したIGF-I遺伝子導入筋芽細胞の挙動解析 (2012)
  • Author(s): 山本昌弘 ら
  • Organizer: 第49回化学関連支部合同九州大会
  • Place of Presentation: 北九州市
• [Presentation] Electrical stimulation culture of artificial skeletal muscle tissue constructs (2012)
  • Author(s): Yasunori Yamamoto et al.
  • Organizer: YABEC2012
  • Place of Presentation: Tokushima, Japan
  • Invited
• [Presentation] IGF-I遺伝子導入による人工筋組織の収縮力の増強 (2011)
  • Author(s): 佐藤暢哲, ら
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大
  • Year and Date: 2011-09-27
• [Presentation] 磁力を用いた細胞積層技術による三次元筋組織の作製 (2011)
  • Author(s): 山本泰徳, ら
  • Organizer: 化学工学会第43回秋季大会
  • Place of Presentation: 東京農工大
  • Year and Date: 2011-09-14
• [Presentation] Functional evaluation of artificial skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique (2011)
  • Author(s): Akira Ito, et al
  • Organizer: TERMIS 2011 AP
  • Place of Presentation: Singapore
  • Year and Date: 2011-08-04
• [Presentation] 熱ストレスによる培養筋組織の機能強化 (2011)
  • Author(s): 菅野翔太、井藤彰、佐藤暢哲、河邉佳典、上平正道
  • Organizer: 化学関連支部合同九州大会
  • Place of Presentation: 北九州市
  • Year and Date: 2011-07-09
• [Presentation] 熱ストレスによる培養筋組織の機能強化 (2011)
  • Author(s): 菅野翔太, ら
  • Organizer: 第48回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2011-07-09
• [Presentation] Bcl-2遺伝子導入によるアポトーシス耐性筋組織の作製 (2011)
  • Author(s): 佐藤暢哲, ら
  • Organizer: 化学工学会第77回年会
  • Place of Presentation: 工学院大学
  • Year and Date: 2011-03-15
• [Presentation] IGF-I遺伝子導入による人工筋組織の収縮力の増強 (2011)
  • Author(s): 佐藤暢哲、井藤彰、河邉佳典、上平正道
  • Organizer: 日本生物工学会
  • Place of Presentation: 東京都
• [Presentation] 磁力を用いた細胞積層技術による三次元筋組織の作製 (2011)
  • Author(s): 山本泰徳、井藤彰、山口勝矢、実延秀昭、河邉佳典、水本博、上平正道
  • Organizer: 化学工学会
  • Place of Presentation: 名古屋市
• [Presentation] Functional Evaluation of Artificial Skeletal Muscle Tissue Constructs Fabricated by a Magnetic Force-Based Tissue Engineering Technique (2011)
  • Author(s): Akira Ito, Yasunori Yamamoto, Yoshinori Kawabe, Masamichi Kamihira
  • Organizer: Tissue Engineering and Regenerative Medicine International Society (TERMIS) 2011 Asia Pacific Meeting
  • Place of Presentation: Singapore
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002906/index.html
• [Remarks]
  • URL: http://www.chem-eng.kyushu-u.ac.jp/lab3/researches.html
• [Remarks] 九州大学 研究者情報 井藤 彰
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002906/index.html
• [Remarks] 九州大学工学研究院上平研究室HP
  • URL: http://www.chem-eng.kyushu-u.ac.jp/lab3/researches.html
• [Remarks] 九州大学上平研HP
  • URL: http://www.chem-eng.kyushu-u.ac.jp/lab3/researches.html
• [Remarks] 九州大学研究者情報
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002906/index.html
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002906/research.html