Elucidation for the mitochondrial tRNA processing mechanism
| Project/Area Number |
23687015
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
NUMATA Tomoyuki 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (10401564)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | tRNA / アンチコドン / 修飾ヌクレオシド / tRNA修飾酵素 / 結晶構造解析 / ミトコンドリア / tRNAプロセシング / RNase P / 生体高分子複合体 / tRNA修飾 / 化学修飾 / GTP結合タンパク質 / RNA / タンパク質 / X線結晶構造解析 |
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| Outline of Final Research Achievements |
Post-transcriptional modifications at the tRNA anticodon loop are essential for precise decoding of the genetic code. The modification defects in mitochondrial tRNA are the main cause of the mitochondrial dysfunction. In this study, I determined the crystal structure of the tRNA modification enzyme, which is responsible for synthesizing the cyclic form of N6-threonylcarbamoyladenosine, as part of elucidating the tRNA modification mechanisms. Together with the structure-based mutational experiments, the amino acid residues crucial for the reaction were also identified.
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Report
(5 results)
Research Products
(14 results)
