| Budget Amount *help |
¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
We newly developed the analytical method for estimating the contents, numbers of segments, sequences, and orientations of protein secondary structures in aqueous solution using a vacuum-ultraviolet circular dichroism (VUVCD) with a synchrotron-radiation light source. This spectroscopy was powerful technique for characterizing the structures of proteins at non-native states such as the membrane-bound state. The VUVCD spectrum of amyloid fibrils was assigned with the CD theory and molecular dynamics simulation to characterize the intermolecular structures of amyloid fibrils which are important for understanding the fibril formations and toxicity. A press release of this research was issued to the media. Further, we invited 14 domestic and international experts of CD fields and organized Hiroshima International Workshop on Circular Dichroism (4th May, 2014, Hiroshima) to promote the usage of protein structural analysis using the VUVCD spectroscopy.
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