Mechanical and thermal properties of living organisms at the mesoscopic scale

• Project/Area Number: 23687021
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Waseda University
• Principal Investigator: SUZUKI Madoka 早稲田大学, 重点領域研究機構, 准教授 (40350475)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000); Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2012: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000); Fiscal Year 2011: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 共焦点顕微鏡 / 生物物理 / 分子モーター / 細胞・組織 / 生体分子 / ナノ材料創製 / ナノ材料評価 / ナノ多機能材料 / 細胞骨格・運動 / 運動・輸送 / バイオイメージング / 細胞情報・動態

Research Abstract

(i) Force (work) and (ii) temperature (heat) are frequently considered as macroscopic parameters. Here we developed novel methods to handle these physical parameters at the single cell scale, and tried to discover biologically important phenomena. (i) A novel cell-sheet system was developed. We examined the response of a cell-sheet to external mechanical stimulus under an optical microscope. (ii) We found the cells respond to heat pulses. Also, we developed novel temperature probes to study single cell thermogenesis.

Research Products

• [Journal Article] A Nanoparticle-Based Ratiometric and Self-Calibrated Fluorescent Thermometer for Single Living Cells (2014)
  • Author(s): Takei, Y., Arai, S., Murata, A., Takabayashi, M., Oyama, K., Ishiwata, S., Takeoka, S., and Suzuki M.
  • Journal Title: ACS Nano. Volume: 8 Pages: 198-206
  • DOI: 10.1021/nn405456e
  • Peer Reviewed
• [Journal Article] 歩くナノ温度計 (2013)
  • Author(s): 鈴木団、大山廣太郎、石渡信一
  • Journal Title: 生物物理 Volume: 53(3) Pages: 158-159
  • NAID: 10031159069
• [Journal Article] Walking nanothermometers : Spatiotemporal temperature measurement of transported acidic organelles in single living cells (2012)
  • Author(s): Oyama, K., Takabayashi, M., Takei, Y., Arai, S., Takeoka, T, Ishiwata, S. and Suzuki, M.
  • Journal Title: Lab Chip Volume: 12 Pages: 1591-1593
• [Journal Article] Mass Spectrometric Screening of Ligands with Lower Off-rate from a Clicked-based Pooled Library (2012)
  • Author(s): Arai, S., Hirosawa, S., Oguchi, Y., Suzuki, M., Murata, A., Ishiwata, S. and Takeoka, S
  • Journal Title: ACS Comb. Sci Volume: 14(8) Pages: 451-455
• [Journal Article] Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients (2012)
  • Author(s): Oyama, K., Mizuno, A., Shintani, S.A., Itoh, H., Serizawa, T., Fukuda, N., Suzuki, M. and Ishiwata, S.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 417 Pages: 607-612
• [Journal Article] Walking nanothermometers: Spatiotemporal temperature measurement of transported acidic organelles in single living cells. (2012)
  • Author(s): Kotaro Oyama
  • Journal Title: Lab Chip Volume: 12 Pages: 1591-1593
  • DOI: 10.1039/c2lc00014h
  • Peer Reviewed
• [Journal Article] Mass Spectrometric Screening of Ligands with Lower Off-rate from a Clicked-based Pooled Library. (2012)
  • Author(s): Arai, S., Ishiwata, S., et al.
  • Journal Title: ACS Combinatorial Science Volume: 14 Pages: 451-455
  • DOI: 10.1021/co300028n
  • Peer Reviewed
• [Journal Article] Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients (2012)
  • Author(s): Kotaro Oyama
  • Journal Title: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 417 Pages: 607-612
  • DOI: 10.1016/j.bbrc.2011.12.015
  • Peer Reviewed
• [Journal Article] Fast temperature measurement following single laser-induced cavitation inside a microfluidic gap. Quinto-Su, P.A.
  • Author(s): Suzuki, M. and Ohl, C.-D
  • Journal Title: Sci. Rep Volume: (in press)
• [Presentation] Thermometry in aqueous solution at single cell-scale using fluorescent nanothermometers (2014)
  • Author(s): Suzuki, M
  • Organizer: 第91回日本生理学会大会(The 91st Annual Meeting of the Physiological Society of Japan)
  • Place of Presentation: Kagoshima, Japan
  • Invited
• [Presentation] Thermometry in aqueous solution at single cell-scale using fluorescent nanothermometers (2014)
  • Author(s): Suzuki, M.
  • Organizer: The 91st Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島
  • Invited
• [Presentation] Thermal activation of cardiac thin filaments induces contraction without intracellular Ca2+ releases: studies with cardiomyocytes and an in vitro motility assay. (2014)
  • Author(s): Oyama, K., Ishii, S., Arai, T., Shintani, S.A., Itoh, H., Fukuda, H., Suzuki, M. and Ishiwata, S.
  • Organizer: Biophysical Society the 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Bleb formation in a HeLa cell induced by temperature gradient. (2014)
  • Author(s): Arai, T., Isaka, A., Oyama, K., Itoh, H., Seto, Y., Suzuki, M. and Ishiwata, S.
  • Organizer: Biophysical Society the 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Intracellular Ca2+ imaging to temperature stimuli on cultured cells. (2014)
  • Author(s): Itoh, H., Oyama, K., Suzuki, M., Lane, E. B. and Ishiwata, S.
  • Organizer: Singapore International Conference on Skin Research
  • Place of Presentation: Singapore
• [Presentation] Microscopic thermometry in a living cell by fluorescent nanoprobes (2014)
  • Author(s): Suzuki, M
  • Organizer: Japan-A*STAR Advanced Diagnostics Forum
  • Place of Presentation: Singapore
  • Year and Date: 2014-03-24
  • Invited
• [Presentation] Single cell-scale thermometry by fluorescent temperature reporters (2013)
  • Author(s): Suzuki, M
  • Organizer: 7th International Symposium on Nanomedicine (ISNM 2013)
  • Place of Presentation: Kyushu Institute of Technology, Kitakyushu, Japan
  • Invited
• [Presentation] Single cell-scale thermometry by fluorescent temperature reporters (2013)
  • Author(s): Suzuki, M.
  • Organizer: 7th International Symposium on Nanomedicine (ISNM 2013)
  • Place of Presentation: 北九州
  • Invited
• [Presentation] Spot-by-spot thermometry in aqueous solution by fluorescent temperature reporters under optical microscope (2013)
  • Author(s): Suzuki, M.
  • Organizer: International Symposium on Nanomedicine Molecular Science (NMMS 2013)
  • Place of Presentation: 東京
• [Presentation] 単一ナノ粒子計測による単一HeLa細胞内の温度依存的な機能活性の測定と活性依存的な熱発生の検出 (2013)
  • Author(s): 鈴木団、新井敏、村田篤、大山廣太郎、高林昌生、武井義明、石渡信一、武岡真司
  • Organizer: 第62回高分子討論会
  • Place of Presentation: 金沢
• [Presentation] Actin filament remodeling in cell-sheet by mechanical stretch (2013)
  • Author(s): Suzuki, M., Kawauchi, K., Chai, E.C., Yamauchi, S., Ishiwata, S. and Fujita, H.
  • Organizer: The 51st Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 京都
• [Presentation] Ca2+-coupled local thermogenesis in a single HeLa cell measured by polymeric nanoparticles as ratiometric nanothermometers. (2013)
  • Author(s): Takei, Y., Arai, S., Murata, A., Takabayashi, M., Oyama, K., Ishiwata, S., Takeoka, S. and Suzuki, M.
  • Organizer: The 5th EMBO Meeting
  • Place of Presentation: Amsterdam, Holland
• [Presentation] Calcium release induced by a microscopic heat pulse in WI-38 fibroblasts. (2013)
  • Author(s): Itoh, H., Oyama, K., Suzuki, M. and Ishiwata, S.
  • Organizer: The 5th EMBO Meeting
  • Place of Presentation: Amsterdam, Holland
• [Presentation] Microscopic heat pulses induce Ca2+-independent contraction of cardiomyocytes. (2013)
  • Author(s): Oyama, K., Mizuno, A., Shintani, S.A., Itoh, H., Serizawa, T., Fukuda, N., Suzuki, M., and Ishiwata S.
  • Organizer: 米国生物物理学会第57回年会
  • Place of Presentation: 米国
• [Presentation] Fluorescent nanoparticle as a temperature sensor in single living cells. (2013)
  • Author(s): Suzuki, M.
  • Organizer: The Taiwan-Japan Nanomedicine Meeting
  • Place of Presentation: 台湾
• [Presentation] Temperature measurement in the scale of single cell with fluorescent temperature reporters. MBI Seminar, Mechanobiology Institute (2013)
  • Author(s): Suzuki, M
  • Organizer: National University of Singapore
  • Place of Presentation: Singapore
  • Year and Date: 2013-09-17
  • Invited
• [Presentation] 一匹の細胞で温度を扱うこと&擬似組織系で細胞の力学負荷応答を見ること (2013)
  • Author(s): 鈴木団
  • Organizer: 「細胞機能究明の最前線3」理研セミナー
  • Place of Presentation: 東京
  • Year and Date: 2013-06-20
  • Invited
• [Presentation] Ca2+-decoupled contraction of single rat adult cardiomyocytes induced by microscopic heat pulse. (2012)
  • Author(s): Oyama, K., Mizuno, A., Shintani, S.A., Itoh, H., Serizawa, T., Fukuda, N., Ishiwata S. and Suzuki, M.
  • Organizer: The American Society for Cell Biology, 2012 Annual Meeting
  • Place of Presentation: 米国
• [Presentation] Development of ratiometric fluorescent nanothermometer and its application to living cells. (2012)
  • Author(s): 武井義明、新井敏、村田篤、大山廣太郎、高林昌生、石渡信一、武岡真司、鈴木団
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Fluorescent Nanoparticles for the Temperature Measurement inside Living Cells with Spatiotemporal Resolution. (2012)
  • Author(s): Suzuki, M.
  • Organizer: The 2012 Small Sciences Symposium -Frontiers in Nanomedicine-
  • Place of Presentation: シンガポール
• [Presentation] Ca2+-independent on-off regulation of a cardiomyocyte by microscopic heat pulses. (2012)
  • Author(s): Oyama, K., Mizuno, A., Shintani, S., Itoh, H., Serizawa, T., Fukuda, N., Suzuki, M., and Ishiwata S.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] Temperature dependency of Ca2+ dynamics in a migrant cell. (2012)
  • Author(s): Itoh, H., Suzuki, M., Oyama, K. and Ishiwata, S.
  • Organizer: Joint Meeting of the 45th Annual Meeting of the Japanese Society of Developmental Biologists and the 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: 神戸
• [Presentation] 局所熱パルスは心筋細胞のカルシウム濃度上昇を伴わない収縮を誘発する (2012)
  • Author(s): Kotaro Oyama,…, Madoka Suzuki, Norio Fukuda, Shin'ichi Ishiwata
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-29
• [Presentation] 細胞内応用を目的としたレシオ型温度感受性蛍光ナノ粒子の構築 (2012)
  • Author(s): 武井義明、…、鈴木団、石渡信一、武岡真司
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶応義塾大学(神奈川)
  • Year and Date: 2012-03-25
• [Presentation] Mitotic spindle in HeLa cell is relocated by the temperature gradient within the cell (2012)
  • Author(s): Suzuki, M.,…Ishiwata, S.
  • Organizer: Biophysical Society the 56th Annual Meeting
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2012-02-25
• [Presentation] Spatiotemporal measurement of the temperature changes in acidic organelles with fluorescent nanothermometers (2012)
  • Author(s): Oyama, K.,…Suzuki, M.
  • Organizer: Biophysical Society the 56th Annual Meeting
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2012-02-25
• [Presentation] Microscopic analysis of calcium dynamics in single migrant cells in response to a heat pulse (2012)
  • Author(s): Itoh, H., Suzuki, M., Oyama, K., Ishiwata, S.
  • Organizer: Biophysical Society the 56th Annual Meeting
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2012-02-25
• [Presentation] メゾスコピック領域でのエネルギー変化測定・撹乱による生物機能の解明と操作に関する研究 (2012)
  • Author(s): Suzuki, M.
  • Organizer: 第4回生物学・化学・情報科学融合のための戦略的研究基盤形成支援事業シンポジウム
  • Place of Presentation: 早稲田大学(東京)
  • Year and Date: 2012-01-21
• [Presentation] 細胞一匹レベルの温度や力 (2012)
  • Author(s): 鈴木団
  • Organizer: 日本定量生物学の会第四回年会
  • Place of Presentation: 名古屋大学(名古屋)
  • Year and Date: 2012-01-07
• [Presentation] 細胞内を輸送される酸性オルガネラの位置と温度の同時計測 (2012)
  • Author(s): 大山廣太郎、…鈴木団
  • Organizer: 2012年生体運動研究合同班会議
  • Place of Presentation: 筑波大学(茨城県)
  • Year and Date: 2012-01-06
• [Presentation] 局所熱パルスで誘起されるCaによらない心筋細胞の収縮 (2012)
  • Author(s): 大山廣太郎、…鈴木団、石渡信一
  • Organizer: 2012年生体運動研究合同班会議
  • Place of Presentation: 筑波大学(茨城県)
  • Year and Date: 2012-01-06
• [Presentation] 光学顕微鏡を用いた局所熱励起・局所温度測定 (2011)
  • Author(s): 鈴木団
  • Organizer: 第10回国際バイオEXPO
  • Place of Presentation: 東京
  • Invited
• [Presentation] 細胞内応用を目的としたレシオ型蛍光ナノ温度計の構築 (2011)
  • Author(s): 武井義明、…鈴木団、石渡信一、武岡真司
  • Organizer: 第33回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-11-21
• [Presentation] Temperature measurement in solution with temporal and spatial resolution (2011)
  • Author(s): Suzuki, M.
  • Organizer: The 2nd Anniversary Symnposium of WABIOS
  • Place of Presentation: バイオポリス(シンガポール)
  • Year and Date: 2011-09-29
• [Presentation] Temperature measurement inside organelles with fluorescent nanothermometers (2011)
  • Author(s): Oyama, K.,…, Suzuki, M.
  • Organizer: The 49th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 姫路
  • Year and Date: 2011-09-16
• [Presentation] Reorganization of mitotic spindle by thermal modulation in HeLa cell (2011)
  • Author(s): Motoyoshi, M., Suzuki, M., Oyama, K., Itabashi, T., Ishiwata, S.
  • Organizer: The 49th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 姫路
  • Year and Date: 2011-09-16
• [Presentation] Sensitivity of Ca2+ dynamics to the heat pulse in migrant cells at various temperatures (2011)
  • Author(s): Itoh, H., Suzuki, M., Oyama, K, Ishiwata, S.
  • Organizer: The 49th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 姫路
  • Year and Date: 2011-09-16
• [Presentation] Remodeling of adherence junction proteins and actin filament in cell sheet by mechanical stimuli (2011)
  • Author(s): Suzuki, M., Fujita, H.
  • Organizer: The EMBO Meeting 2011
  • Place of Presentation: ウィーン(オーストリア)
  • Year and Date: 2011-09-10
• [Presentation] ヒト胎児肺線維芽細胞(WI-38)のカルシウムイオン変動に局所的熱刺激がもたらす効果 (2011)
  • Author(s): 伊藤秀城、鈴木団、大山廣太郎、石渡信一
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-06-27
• [Presentation] High-speed Thermography of Laser Induced Cavitation Bubbles (2011)
  • Author(s): Suzuki, M., Quinto Su, P.A., Ohl, C.D.
  • Organizer: International Conference on Materials for Advanced Technolgies 2011
  • Place of Presentation: サンテック会議場(シンガポール)
  • Year and Date: 2011-06-26
• [Presentation] Microscopic thermometry in a living cell by fluorescent nanoprobes
  • Author(s): Suzuki, M.
  • Organizer: Japan-A*STAR Advanced Diagnostics Forum
  • Place of Presentation: Singapore
  • Invited
• [Presentation] Development and biological applications of fluorescence thermometry under optical microscope.
  • Author(s): Suzuki, M.
  • Organizer: Waseda University-Singapore Polytechnic Inaugural Joint Biomedical Symposium 2013
  • Place of Presentation: Singapore
• [Presentation] Temperature measurement in the scale of single cell with fluorescent temperature reporters
  • Author(s): Suzuki, M.
  • Organizer: MBI Seminar
  • Place of Presentation: Singapore
  • Invited
• [Presentation] 一匹の細胞で温度を扱うこと＆擬似組織系で細胞の力学負荷応答を見ること
  • Author(s): 鈴木団
  • Organizer: 「細胞機能究明の最前線３」理研セミナー
  • Place of Presentation: 東京
  • Invited
• [Presentation] 見えないパラメータに着目した細胞生物物理学－ 温度（熱）と力（仕事）－
  • Author(s): 鈴木団
  • Organizer: ナノメディシン分子科学第二回若手の会
  • Place of Presentation: 京都
• [Presentation] Microscopic analysis of calcium dynamics to various temperature stimuli in a WI-38 cell.
  • Author(s): Itoh, H., Suzuki, M., Oyama, K. and Ishiwata, S.
  • Organizer: 卓越した大学院拠点形成支援事業 スタートアップ・シンポジウム
  • Place of Presentation: 東京
• [Presentation] Development of walking nano-*-meters.
  • Author(s): Suzuki, M.
  • Organizer: ナノメディシン分子科学 公開国際シンポジウム「細胞内の分子世界を探る：化学・物理・生物学の統合による医療革命を目指して」
  • Place of Presentation: 神戸
• [Presentation] メゾスコピック領域でのエネルギー変化測定・撹乱による生物機能の解明と操作に関する研究
  • Author(s): 鈴木団
  • Organizer: 第5回生物学・化学・情報科学融合のための戦略的先進理工学研究基盤の形成事業シンポジウム「ライフイノベーション創出に向けたフュージョンバイオテクノロジー展開」－融合の展開と実践－
  • Place of Presentation: 東京
• [Remarks] 報道関連情報、早稲田大プレスリリース「水中で触れずに細胞の中の温度を測れるレシオ型ナノ温度計を報道関連情報、開発ミクロンサイズの温度変化を測定するナノ温度計の手法を発展」2014年1月17日
• [Remarks] 報道関連情報、日経産業新聞「水中の細胞温度触れず正確測定早大が装置開発」2014年1月21日
• [Remarks] 報道関連情報、マイナビニュース「早稲田、水中で触れずに細胞の温度を測定する「レシオ型ナノ温度計」開発」2014年1月28日
• [Remarks] 報道関連情報、早稲田大プレスリリース「細胞内を歩くナノ温度計を開発ミクロンサイズの細胞内小器官内部の温度変化を世界で初めて測定」2012年3月15日
• [Remarks] 報道関連情報、マイナビニュース「早大、細胞内の局所的で微小な温度変化を測定可能な「歩くナノ温度計」開発」2012年3月14日
• [Remarks] 報道関連情報、日刊工業新聞「細胞内で0.3度C差測定早大ナノ粒子の温度計」2012年3月19日
• [Remarks] 報道関連情報、科学新聞「細胞内を歩く「ナノ温度計」開発ミクロンサイズの細胞内小器官内部の温度変化-早大が世界で初めて測定-」2012年3月23日
• [Remarks]
  • URL: http://www.waseda.jp/jp/news11/120315_nanothermo.html