| Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
We developed a system using CMOS sensor array to detect extracellular action potential. Since CMOS sensor array consists of huge number of electrode, if cells directly contact sensor array, extracellular action potentials of neuronal network comprehensively can be detected. To detect action potential on the sensor array, we selected a sensor array of high-speed camera without photodiode array and micro lens array. As the result, coating with silicone polymer prevented the crush by culture medium and cells could be cultivated on the sensor array. Furthermore, sensor array filled with culture medium could detected electrical change generated with function generator. This result indicated the potential to detect the extracellular action potential.
Furthermore, we developed a new technique to adhere matured cells on the sensor array using calcium alginate gel. Using this technique, neurons could be detach from culture dish and recultivate without destructing synaptic connection.
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