Development of on-chip neuronal network measurement device using nanotechnology

• Project/Area Number: 23687023
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Tokyo Medical and Dental University (2013-2014); Kanagawa Academy of Science and Technology (2011-2012)
• Principal Investigator: TERAZONO Hideyuki 東京医科歯科大学, 生体材料工学研究所, 助教 (30398143)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: センサアレイ / 神経ネットワーク / ナノ加工 / オンチップ / CMOS / 細胞ネットワーク / ナノ加工技術 / 神経細胞 / 細胞外電位計測 / センサアレイ計測 / 細胞外電位記録 / CCD

Outline of Final Research Achievements

We developed a system using CMOS sensor array to detect extracellular action potential. Since CMOS sensor array consists of huge number of electrode, if cells directly contact sensor array, extracellular action potentials of neuronal network comprehensively can be detected. To detect action potential on the sensor array, we selected a sensor array of high-speed camera without photodiode array and micro lens array. As the result, coating with silicone polymer prevented the crush by culture medium and cells could be cultivated on the sensor array. Furthermore, sensor array filled with culture medium could detected electrical change generated with function generator. This result indicated the potential to detect the extracellular action potential.
Furthermore, we developed a new technique to adhere matured cells on the sensor array using calcium alginate gel. Using this technique, neurons could be detach from culture dish and recultivate without destructing synaptic connection.

Research Products

• [Presentation] Development of an artificial neuronal sheet technique and electrophysiological evaluation between neuronal sheets (2015)
  • Author(s): Hideyuki Terazono, Fumimasa Nomura, Hyonchol Kim, Akihiro Hattori, Kenji Yasuda
  • Organizer: 第38回 日本神経科学大会 - 日本神経科学学会
  • Place of Presentation: 兵庫県神戸市神戸国際会議場
  • Year and Date: 2015-07-28 – 2015-07-31
• [Presentation] Constructive approach to understand neuronal communication by controlling spatial patterns using micro-processing and cell-sheet technique (2015)
  • Author(s): TERAZONO Hideyuki, NOMURA Fumimasa, HATTORI Akihiro and YASUDA Kenji
  • Organizer: 2015 Bridging Biomedical Worlds conference
  • Place of Presentation: 東京都千代田区飯野ホール
  • Year and Date: 2015-05-11 – 2015-05-12
• [Presentation] Toward quasi-in vivo from in vitro assay (IV). Development of the artificial neuronal networks system using actual neurons toward to evaluate effects of medicines for Neuropsychiatric disorders (2013)
  • Author(s): Hideyuki Terazono, Hyonchol Kim, Fumimasa Nomura, Tomoyo Hamada, Kenji Yasuda
  • Organizer: SPS 2013
  • Place of Presentation: ロッテルダム, オランダ