Contribution of transcriptional corepressor ETO2 to the epigenome formation in erythroblasts
Project/Area Number |
23687024
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Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Single-year Grants |
Research Field |
Molecular biology
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Research Institution | Tohoku University |
Principal Investigator |
FUJIWARA Tohru 東北大学, 大学院・医学系研究科, 助教 (60333796)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
|
Keywords | 転写 / 遺伝子 |
Research Abstract |
Overexpression of ETO2 as well as chromatin immunoprecipitation analysis in K562 human erythroid cell line revealed that ETO2 directly represses representative erythroid genes, including globin genes. Subsequently, we established erythroid differentiation system from cord blood CD34-positive cells. By using the system, we demonstrated that short hairpin RNA (shRNA)-mediated knockdown of ETO2 in cord blood CD34-positive cell-derived primary erythroblasts resulted in the significantupregulation of globin genes.
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Report
(3 results)
Research Products
(10 results)