Contribution of transcriptional corepressor ETO2 to the epigenome formation in erythroblasts

• Project/Area Number: 23687024
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Tohoku University
• Principal Investigator: FUJIWARA Tohru 東北大学, 大学院・医学系研究科, 助教 (60333796)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 転写 / 遺伝子

Research Abstract

Overexpression of ETO2 as well as chromatin immunoprecipitation analysis in K562 human erythroid cell line revealed that ETO2 directly represses representative erythroid genes, including globin genes. Subsequently, we established erythroid differentiation system from cord blood CD34-positive cells. By using the system, we demonstrated that short hairpin RNA (shRNA)-mediated knockdown of ETO2 in cord blood CD34-positive cell-derived primary erythroblasts resulted in the significantupregulation of globin genes.

Research Products

• [Journal Article] Role of transcriptional corepressor ETO2 in erythroid cells (2013)
  • Author(s): Fujiwara T, Alqadi YW, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Harigae H
  • Journal Title: Experimental Hematology Volume: 41 Issue: 3 Pages: 303-315
  • DOI: 10.1016/j.exphem.2012.10.015
  • Peer Reviewed
• [Journal Article] Gene Expression Profiling Identifies HOXB4 as a Direct Downstream Target of GATA-2 in Human CD34+ Hematopoietic Cells (2012)
  • Author(s): Fujiwara T, Yokoyama H, Okitsu Y, Kamata M, Fukuhara N, Onishi Y, Fujimaki S, Takahashi S, Ishizawa K, Bresnick E and Harigae H
  • Journal Title: PLoS ONE Volume: 7 Issue: 9 Pages: e40959-e40959
  • DOI: 10.1371/journal.pone.0040959
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of GATA transcription factor in hematopoietic cells (2012)
  • Author(s): Fujiwara T
  • Journal Title: Rinsho Ketsueki Volume: 53 Issue: 3 Pages: 287-294
  • DOI: 10.11406/rinketsu.53.287
  • NAID: 130004501644
  • ISSN: 0485-1439, 1882-0824
  • Peer Reviewed
• [Presentation] Regulation of erythropoiesis by histone methyltransferase EZH2 inhibitor 3-Deazaneplanocin A (DZNep) (2012)
  • Author(s): Fujiwara T, Saitoh H, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Ichinohasama R, Harigae H
  • Organizer: 米国血液学会
  • Place of Presentation: アト ランタ(米国)
  • Year and Date: 2012-12-08
• [Presentation] Exploring the mechanism of ETO2-dependent transcriptional regulation in erythroid cells (2011)
  • Author(s): Yarob Wael Alquadi, Tohru Fujiwara, et al
  • Organizer: 米国血液学会
  • Place of Presentation: 米国・サンディエゴ
  • Year and Date: 2011-12-12
• [Presentation] Exploring the mechanism of ETO2-dependent transcriptional regulation in erythroid cells (2011)
  • Author(s): Wael YA, Fujiwara T, Okitsu Y, Yasushi O, Ishizawa K, Harigae H
  • Organizer: 米国血液学会
  • Place of Presentation: サン ディエゴ(米国)
  • Year and Date: 2011-12-11
• [Presentation] Expression profiling ofETO2-regulated genes in erythroid cells (2011)
  • Author(s): Wael YA, Fujiwara T, Okitsu Y, Kohata K, Fukuhara N, Yasushi O, Yamamoto J, Ishizawa K, Harigae H
  • Organizer: 日本血液学会
  • Place of Presentation: 名古 屋
  • Year and Date: 2011-10-16
• [Presentation] Expression profiling of ETO2-regulated genes in erythroid cells (2011)
  • Author(s): Yarob Wael Alquadi, Tohru Fujiwara, et al
  • Organizer: 第73回日本血液学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-16
• [Presentation] Role of ETO2 in the epigenetic regulation of erythroid genes (2011)
  • Author(s): Fujiwara T, Wael YA, Harigae H
  • Organizer: 欧州血液学会
  • Place of Presentation: ロンドン(英国)
  • Year and Date: 2011-06-11
• [Remarks]
  • URL: http://www.rh.med.tohoku.ac.jp/