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Selective degradation of proteins and organelles during early embryogenesis

Research Project

Project/Area Number 23687027
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionGunma University

Principal Investigator

SATO Miyuki  群馬大学, 生体調節研究所, 准教授 (70321768)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Keywordsオートファジー / エンドサイトーシス / リソソーム / 分解 / 受精 / 線虫 / ミトコンドリア / ユビキチン / 母性遺伝 / タンパク質分解 / オルガネラ / 発生
Outline of Final Research Achievements

Fertilization triggers cell remodeling from each gamete to a totipotent zygote. Using C. elegans as a model system, we have shown that lysosomal degradation pathways play important roles in cellular remodeling during this developmental transition. Endocytosis and autophagy, two pathways leading to the lysosomes, are highly upregulated during this period. A subset of maternal membrane proteins is selectively endocytosed and degraded in the lysosomes before the first mitotic cell division. UBC-13-dependent K63-linked ubiquitination is required for the proper sorting of membrane proteins. Autophagy is also induced shortly after fertilization and executes the degradation of paternally inherited embryonic organelles, e.g. mitochondria and membranous organelles. This mechanism underlies the maternal inheritance of the mitochondrial genome. Our study revealed physiological roles of lysosomal pathways during early development.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (41 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 1 results) Presentation (21 results) (of which Invited: 7 results) Remarks (5 results)

  • [Journal Article] Guidelines for monitoring autophagy in Caenorhabditis elegans2015

    • Author(s)
      Zhang H, Chang JT, Guo B, Hansen M, Jia K, Kovacs AL, Kumsta C, Lapierre LR, Legouis R, Lin L, Lu Q, Melendez A, O'Rourke EJ, Sato K, Sato M, Wang X, Wu F
    • Journal Title

      Autophagy

      Volume: 11 Pages: 9-27

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caenorhabditis elegans chaperonin CCT/TRiC is required for actin and tubulin biogenesis and microvillus formation in intestinal epithelial cells2014

    • Author(s)
      Keiko Saegusa, Miyuki Sato, Katsuya Sato, Junko Nakajima-Shimada, Akihiro Harada, and Ken Sato
    • Journal Title

      Mol. Biol. Cell

      Volume: 25 Issue: 20 Pages: 3095-3104

    • DOI

      10.1091/mbc.e13-09-0530

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Fertilization-induced K63-linked ubiquitylation mediates clearance of maternal membrane proteins.2014

    • Author(s)
      Miyuki Sato, Ryosuke Konuma, Katsuya Sato, Kotone Tomura, Ken Sato
    • Journal Title

      Development

      Volume: 141 Issue: 6 Pages: 1324-1331

    • DOI

      10.1242/dev.103044

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 受精における精子ミトコンドリアの運命と母性遺伝2014

    • Author(s)
      佐藤健,佐藤美由紀
    • Journal Title

      細胞工学

      Volume: 33 Pages: 414-419

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Maternal inheritance of mitochondrial DNA by diverse mechanisms to eliminate paternal mitochondrial DNA2013

    • Author(s)
      Miyuki Sato and Ken Sato
    • Journal Title

      Biochim Biophys Acta

      Volume: 1833 Issue: 8 Pages: 1979-1984

    • DOI

      10.1016/j.bbamcr.2013.03.010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ミトコンドリアDNAの母性遺伝を制御する多様な分子機構2013

    • Author(s)
      佐藤美由紀,佐藤健
    • Journal Title

      生化学

      Volume: 85 Pages: 357-362

    • NAID

      10031177329

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Dynamic regulation of autophagy and endocytosis for cell remodeling during early development.2013

    • Author(s)
      Miyuki Sato
    • Journal Title

      Traffic

      Volume: 14 Issue: 5 Pages: 479-486

    • DOI

      10.1111/tra.12050

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 受精卵における細胞内リモデリングのメカニズム.2013

    • Author(s)
      佐藤美由紀
    • Journal Title

      女性科学者の会 学術誌

      Volume: 13 Pages: 9-13

    • NAID

      130003379023

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 精子由来ミトコンドリアは受精依存的に誘導されるオートファジーによって選択的に分解される.2012

    • Author(s)
      佐藤健
    • Journal Title

      細胞工学

      Volume: 31 Pages: 590-591

    • Related Report
      2012 Annual Research Report
  • [Journal Article] ミトコンドリアゲノムの母性遺伝のメカニズム.2012

    • Author(s)
      佐藤美由紀
    • Journal Title

      化学と生物

      Volume: 50 Pages: 479-480

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 動物におけるミトコンドリアDNAの母性遺伝の分子機構.2012

    • Author(s)
      佐藤健
    • Journal Title

      生体の科学

      Volume: 63 Pages: 436-437

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Maternal inheritance of mitochondrial DNA : Degradation of paternal mitochondria by allogeneic organelle autophagy, allophagy2012

    • Author(s)
      Miyuki Sato
    • Journal Title

      Autophagy

      Volume: 8 Issue: 3 Pages: 424-425

    • DOI

      10.4161/auto.19243

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 線虫受精卵における父性ミトコンドリアのオートファジーによる選択的分解-ミトコンドリアDNAの母性遺伝のメカニズム-2012

    • Author(s)
      佐藤美由紀
    • Journal Title

      実験医学

      Volume: 30 Pages: 614-618

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Degradation of paternal mitochondria by fertilization-triggered autophagy in C.elegans embryos2011

    • Author(s)
      Miyuki Sato
    • Journal Title

      Science

      Volume: 334 Issue: 6059 Pages: 1141-1144

    • DOI

      10.1126/science.1210333

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caenorhabditis elegans SNAP-29 isrequired for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells.2011

    • Author(s)
      Sato M, Saegusa K, Sato K, Hara T, Harada A, Sato K.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 22 Issue: 14 Pages: 2579-2587

    • DOI

      10.1091/mbc.e11-04-0279

    • NAID

      120004898416

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 線虫 C.elegans においてシャペロニン CCT は微絨毛の形態形成に重要な役割を果たす2015

    • Author(s)
      三枝慶子,佐藤美由紀,佐藤克哉,嶋田淳子,原田彰宏,佐藤健.
    • Organizer
      第13回群馬大学大学院医学系研究科・大学院生によるワークショップ
    • Place of Presentation
      群馬大学医学部
    • Year and Date
      2015-02-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamic Regulation of Autophagy and Endocytosis for Cell Remodeling during Early Development in C. elegans2014

    • Author(s)
      Miyuki Sato, Ryosuke Konuma, Kotone Tomura, Ken Sato
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 低分子量GTPase RAB-11の新規結合因子REI-1/REI-2は受精後のRAB-11再局在化を制御する2014

    • Author(s)
      坂口 愛沙,佐藤 美由紀,安藤 恵子,佐藤 克哉,中井 淳一,佐藤 健
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 線虫受精卵における父性オルガネラの選択的分解に関与する新規因子の探索2014

    • Author(s)
      佐藤美由紀
    • Organizer
      第8 回オートファジー研究会
    • Place of Presentation
      シャトレーゼ ガトーキングダム サッポロ
    • Year and Date
      2014-11-10 – 2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 初期胚におけるリソソーム分解系の生理機能 ~エンドサイトーシスとオートファジーによる膜成分の再編成~2014

    • Author(s)
      佐藤美由紀
    • Organizer
      群馬大学 大学院理工学府・生体調節研究所 第19回生命科学セミナー
    • Place of Presentation
      群馬大学医学部
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Fertilization-induced K63-linked ubiquitination mediates clearance of maternal membrane proteins2014

    • Author(s)
      Miyuki Sato, Ryosuke Konuma, Katsuya Sato, Kotone Tomura, Ken Sato
    • Organizer
      C. elegans Development, Cell Biology and Gene Expression Meeting in association with the 6th Asis-Pacific C. elegans Meeting
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2014-07-15 – 2014-07-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Novel binding partner of small GTPase RAB-11 regulates RAB-11 redistribution to Golgi after fertilization2014

    • Author(s)
      Aisa Sakaguchi, Miyuki Sato, Keiko Gengyo-Ando, Katsuya Sato, Junichi Nakai, Ken Sato
    • Organizer
      C. elegans Development, Cell Biology, Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2014-07-15 – 2014-07-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] C. elegans chaperonin CCT/TRiC is required for actin biogenesis and microvillus formation in intestinal epithelial cells2014

    • Author(s)
      Keiko Saegusa, Miyuki Sato, Katsuya Sato, Taichi Hara, Akihiro Harada, and Ken Sato
    • Organizer
      C. elegans Development, Cell Biology, Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2014-07-15 – 2014-07-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 線虫受精卵においてダイナミックに変化するRAB-11の動態制御機構の解析2014

    • Author(s)
      坂口 愛沙,佐藤 美由紀,安藤 恵子,佐藤 克哉,中井 淳一,佐藤 健
    • Organizer
      日本細胞生物学会年会
    • Place of Presentation
      奈良県新公会堂・東大寺総合文化センター
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 父性ミトコンドリアの選択的オートファジーの分子機構解明を目指して2014

    • Author(s)
      佐藤美由紀
    • Organizer
      YoungMito2014
    • Place of Presentation
      大阪府 箕面山荘
    • Year and Date
      2014-05-22 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 受精後に誘導されるエンドサイトーシスを介した細胞膜成分の再編成のメカニズム2013

    • Author(s)
      佐藤美由紀,佐藤健
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      ウインクあいち
    • Related Report
      2013 Annual Research Report
  • [Presentation] オートファジーによる父性ミトコンドリアの選択的分解と母性遺伝2013

    • Author(s)
      佐藤美由紀,塚本智史,原太一,水島昇,佐藤健
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Mechanism of selective degradation of paternal mitochondria by autophagy2013

    • Author(s)
      Shota Tomizawa, Miyuki Sato, Kotone Tomura, Ken Sato
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 受精における表層顆粒の分泌を制御するRAB-11に結合する新規因子の解析2013

    • Author(s)
      坂口愛沙,佐藤美由紀,安藤恵子,佐藤克哉,中井淳一,佐藤健
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 受精卵におけるオートファジーとエンドサイトーシスの生理機能2013

    • Author(s)
      佐藤 美由紀
    • Organizer
      細胞内ロジスティクス・シンポジウム
    • Place of Presentation
      淡路夢舞台
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 受精によって誘導されるオートファジーの分子機構解明を目指して2013

    • Author(s)
      佐藤 美由紀
    • Organizer
      第7回オートファジー研究会
    • Place of Presentation
      ヤマハリゾートつま恋
    • Related Report
      2013 Annual Research Report
  • [Presentation] Degradation of paternal mitochondria by fertilization-triggered autophagy in C. elegans embryos2012

    • Author(s)
      佐藤美由紀
    • Organizer
      Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Metting of the Japan Society for Cell Biology
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Fertilization-triggered autophagy degrades paternal mitochondria in C. elegans early embryos2012

    • Author(s)
      佐藤美由紀
    • Organizer
      第85回生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Degradation of paternal mitochondria by fertilization-triggered autophagy in C. elegans embryos2012

    • Author(s)
      佐藤美由紀
    • Organizer
      The 6th International Symposium on Autophagy 2012
    • Place of Presentation
      沖縄万国津梁館
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Fertilization triggers selective degradation of membrane proteins and organelles in C.elegans embryos2011

    • Author(s)
      佐藤美由紀
    • Organizer
      第34回日本分子生物学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Selective degradation mechanisms of meiotic membrane proteins in C.elegans embryos2011

    • Author(s)
      佐藤健
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)(招待講演)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Remarks] 群馬大学生体調節研究所生体膜機能分野HP

    • URL

      http://makukinou.showa.gunma-u.ac.jp/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 群馬大学生体調節研究所細胞構造分野HP

    • URL

      http://traffic.dept.med.gunma-u.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] http://traffic.dept.med.gunma-u.ac.jp/index

    • Related Report
      2013 Annual Research Report
  • [Remarks] 群馬大学生体調節研究所細胞構造分野HP

    • URL

      http://traffic.dept.med.gunma-u.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://traffic.dept.med.gunma-u.ac.jp/reserch7.html

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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