| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Research Abstract |
The multi-protein complex TORC1 is a key regulator of cell growth and size control in both Drosophila and mammals. To better understand amino acid signaling, we took a biochemical approach to identify the binding proteins of small GTPase RagA/C. We identified the glycolytic enzyme GAPDH and found that GAPDH likely regulates the TORC1 localization and activity through RagA/C.
In addition, to understand how Drosophila recognizes nutrition to control body growth, we focused on the molecular mechanism underlying the nutrient-dependent expression of Drosophila Insulin-like peptide (dilp). We identified the responsible transcription factors that cooperatively control dilp5 gene expression. We also conducted in vivo RNAi screening to identify novel players for the regulation of body growth. We identified a gene that we named SDR. SDR is a new class of secreted Dilp-binding proteins that negatively regulate the function of Dilps and thereby body growth.
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