| Budget Amount *help |
¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Research Abstract |
We studied mechanisms of early embryogenesis in a cricket, a model system of hemimetabolous insects. We performed RNAi analyses for components of signaling pathways, such as Wnt and BMP pathways, using GFP-expressing transgenic embryos for visualizing cellular dynamics. We found that Wnt signaling is involved in regulation of direction of movements of presumptive embryonic cells, and that BMP signaling is required for cell migration from the dorsal side towards the ventral side. We also showed that these cell signalings are involved in regulation of expression of pattern formation genes, such as otd. On the other hand, we developed gene knock-out and knock-in techniques using genome editing technologies in the cricket. In addition to progress of whole genome sequencing, these techniques provides us strong tools for further studies based on various strategies.
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