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Construction of an experimental model system that mimic the evolution of early life

Research Project

Project/Area Number 23687034
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionOsaka University

Principal Investigator

ICHIHASHI Norikazu  大阪大学, 情報科学研究科, 准教授 (20448096)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords進化 / 人工ゲノム / 実験進化 / RNAゲノム / ダーウィン進化 / 人工細胞 / 生命の起源 / 自己複製 / 無細胞翻訳系 / エマルション
Research Abstract

The evolutionary process from the assembly of chemical compounds to the present-day life is largely unknown. One of the major obstacles is the lack of the experimental model. In this project, we have succeeded in the construction of an experimental model that functionally mimic the early life and have the ability of autonomous evolution. This system would be useful for the experimental investigation of the evolutionary process from a simple genome replication system to a present-day complex life system.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (35 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (21 results) (of which Invited: 2 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Effects of ribosomes on the kinetics of Qb replication2014

    • Author(s)
      Usui, K., Ichihashi, N., Kazuta, Y., Matsuura, T., Yomo, T
    • Journal Title

      FEBS Letters

      Volume: 588 Issue: 1 Pages: 117-123

    • DOI

      10.1016/j.febslet.2013.11.018

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment2013

    • Author(s)
      Ichihashi, N., Usui, K., Kazuta, Y., Sunami, T., Matsuura T., Yomo, T
    • Journal Title

      Nature Communications

      Volume: 4 Issue: 1 Pages: 1-7

    • DOI

      10.1038/ncomms3494

    • NAID

      120006831817

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kinetic model of double-stranded RNA formation during long RNA replication by Qβreplicase2013

    • Author(s)
      Usui, K., Ichihashi, N., Kazuta, Y., Matsuura, T., Yomo, T
    • Journal Title

      FEBS Letters

      Volume: 587 Issue: 16 Pages: 2565-2571

    • DOI

      10.1016/j.febslet.2013.06.033

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Probabilistic model based error correction in a set of various mutant sequences analyzed by next-generation sequencing.2013

    • Author(s)
      Takuyo Aita, Norikazu Ichihashi, Tetsuya Yomo.
    • Journal Title

      Computational Biology and Chemistry

      Volume: 47 Pages: 221-230

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A controllable gene expression system in liposomes that includes a positive feedback loop.2013

    • Author(s)
      Kobori S
    • Journal Title

      Mol Biosyst.

      Volume: 印刷中

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of compartment size on the kinetics of intracompartmental multimeric orotein synthesis2012

    • Author(s)
      Matsuura, T., Hosoda, K., Kazuta, Y., Ichihashi, N., Suzuki, H., Yomo, T
    • Journal Title

      ACS Synthetic Biology

      Volume: 1 Issue: 9 Pages: 431-437

    • DOI

      10.1021/sb300041z

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kinetic analysis of aptazyme-regulated gene expression in a cell-fiee translation system:modeling of ligand-dependent and -independent expression2012

    • Author(s)
      小堀 峻吾
    • Journal Title

      RNA

      Volume: 18 Issue: 8 Pages: 1458-1465

    • DOI

      10.1261/rna.032748.112

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] α-Complementation in an artificial genome replication system in liposomes2012

    • Author(s)
      Nishiyama, K., Ichihashi, N., Matsuura, T., Kazuta, Y., Yomo, T
    • Journal Title

      Chembiochem

      Volume: 13 Issue: 18 Pages: 2701-2706

    • DOI

      10.1002/cbic.201200586

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Importance of parasite RNA species repression for prolonged translation-coupled RNA self-replication2012

    • Author(s)
      Y Bansho, Y., Ichihashi, N., Kazuta, Y., Matsuura, T., Suzuki, H., Yomo, T
    • Journal Title

      Chemistry and Biology

      Volume: 19 Issue: 4 Pages: 478-487

    • DOI

      10.1016/j.chembiol.2012.01.019

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of Gene Screening System Using Giant Unilamellar Liposomes and Fluorescence Activated Cell Sorter2012

    • Author(s)
      T. Nishikawa、T. Sunami, T. Matsuura, N. Ichihashi, T. Yomo
    • Journal Title

      Analytical Chemistry

      Volume: 84 Issue: 11 Pages: 5017-5024

    • DOI

      10.1021/ac300678w

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] The International Astrobiology Workshop 20132013

    • Author(s)
      Ichihashi, N
    • Organizer
      as the Japan Astrobiology Network Annual Meeting #6
    • Place of Presentation
      JAXA/ISAS,Sagamihara, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Darwinian Evolution in a Translation-Coupled RNA Replication System Within a Cell-Like Compartment.2013

    • Author(s)
      Norikazu Ichihashi, Kimihito Usui, Tetsuya Yomo.
    • Organizer
      The International Astrobiology Workshop 2013(as the Japan Astrobiology Network Annual Meeting #6)
    • Place of Presentation
      JAXA/ISAS, Sagamihara, Japan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 人工自己複製システムにおける利己的RNAの出現と耐性の進化2013

    • Author(s)
      市橋伯一, 四方哲也
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 次世代シーケンサーを用いた人工細胞モデルにおけるゲノムRNAの進化プロセスの解析2013

    • Author(s)
      市橋伯一, 中村昇太 , 四方哲也
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] PURE SYSTEMを用いた人工RNAゲノムの自己複製・進化システムの構築2013

    • Author(s)
      市橋伯一, 臼井公人, 四方哲也
    • Organizer
      第8回無細胞生命科学研究会
    • Place of Presentation
      新潟大学中央図書館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Continuous RNA amplification with self-encoded replication system.2012

    • Author(s)
      Kimihito Usui, et al
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Investigation on the mechanism of double strand formation during RNA replication.2012

    • Author(s)
      Yohei Uegami, et al
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] In Vitro Compartmentalization Using Unilamellar Lipsomes for Gene Screening.2012

    • Author(s)
      Takehiro Nishikawa, et al
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Directed evolution of a self-encoding system using giant liposome.2012

    • Author(s)
      Takeshi Sunam, et al
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] アプタザイムを用いた外部応答するリアクターの構築2012

    • Author(s)
      小堀峻吾, 他
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Kinetic Analysis of Aptazyme-regulated Gene Expression in a Cell-free Translation System.2012

    • Author(s)
      Shungo Kobori, et al
    • Organizer
      RNA 2012 PROGRAM & ABSTRACTS The 17th Annual Meeting
    • Place of Presentation
      Michigan, USA
    • Year and Date
      2012-06-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Kinetic Analysis of the Double-stranded RNA Formation in Qβ RNA Replication.2012

    • Author(s)
      Kimihito Usui, et al
    • Organizer
      RNA 2012 PROGRAM & ABSTRACTS The 17th Annual Meeting
    • Place of Presentation
      Michigan, USA
    • Year and Date
      2012-05-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] リボソームを反応場とする遺伝子スクリーニングによる活性型グルクロニダーゼの探索2012

    • Author(s)
      西川雄大, 他
    • Organizer
      第61回高分子学会年次大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-05-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] The Thirteenth International Conference on the Synthesis and Simulation of Living Systems2012

    • Author(s)
      Ichihashi, N
    • Organizer
      Artificial Life XIII
    • Place of Presentation
      Michigan State University in East Lansing, Michigan, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Darwinian Evolution of Translation-coupled RNA Self-replication System.2012

    • Author(s)
      Ichihashi N
    • Organizer
      The Thirteenth International Conference on the Synthesis and Simulation of Living Systems (Artificial Life XIII)
    • Place of Presentation
      Michigan State University in East Lansing, Michigan, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] 人工自己複製システムのダーウィン進化2012

    • Author(s)
      市橋伯一
    • Organizer
      「細胞を創る」研究会5.0
    • Place of Presentation
      神奈川県 東京工業大学すずかけ台キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] 人工自己複製RNAの実験室内進化2012

    • Author(s)
      市橋伯一
    • Organizer
      日本進化学会第14回東京大会
    • Place of Presentation
      東京都 首都大学東京南大沢キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] 人工自己複製システムのダーウィン進化2012

    • Author(s)
      市橋伯一
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      愛知県 名古屋大学 東山キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] Creating an artificial cell with different size revealed the effect of compartment volume on the intracompartmental multimeric protein synthesis2012

    • Author(s)
      Tomoaki Matsuura, et al
    • Organizer
      The Thirteenth International Conference on the Synthesis and Simulation of Living Systems (Artificial Life XIII)
    • Place of Presentation
      Michigan, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] Darwinian Evolution of Translation-coupled RNA Self-replication System.2012

    • Author(s)
      Norikazu Ichihashi, et al
    • Organizer
      The Thirteenth International Conference on the Synthesis and Simulation of Living Systems (Artificial Life XIII)
    • Place of Presentation
      Michigan, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] RNA自己複製反応の反応場体積依存性2011

    • Author(s)
      番所洋輔, 他
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Book] 進化分子工学 高速分子進化によるタンパク質・核酸の開発2013

    • Author(s)
      角南武志, 市橋伯一, 四方哲也
    • Total Pages
      466
    • Publisher
      NTS
    • Related Report
      2013 Annual Research Report
  • [Book] Constructive approaches for the origin of life. A captor in"Genesis - in the Beginning : Precursors of Life, Chemical Models and Early Biological Evolution."2012

    • Author(s)
      Ichihashi, N., Matsuura, T., Kita, H., Sunami, T., Suzuki, H., Yomo, T(J. Seckbach Ed)
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] Genesis - in the Beginning: Precursors of Life, Chemical Models and Early Biological Evolution. (分担)2012

    • Author(s)
      Norikazu Ichihashi, et al
    • Total Pages
      973
    • Publisher
      Springer
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www-symbio.ist.osaka-u.ac.jp/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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