| Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2011: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Research Abstract |
To develop DNA markers, we sequence Azuki bean genome by next generation sequencer. The assembled sequences covered more than 90 % of the genome.
With the genome sequence information, we fin-mapped the mapping population derived from Vigna species. We identified two useful genes, mog and ptw. The mog gene makes the seed size doubled, while the ptw gene controls seed pod twisting, which is related to seed pod dehiscence.
We also tried to screen EMS-treated M2 population of V. stiplucacea, which is pests and diseases resistant. As a result, we found 5 lines with bigger seed size and one line with non-seed pod dehiscence. With all these results, we considered domestication of wild Vigna species is a quick and practical approach to develop a stress-tolerant crop.
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