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Improvement of P uptake from unavailable forms in the soil by enhancement of secreting function of organic acids and phosphatase

Research Project

Project/Area Number 23688010
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionHiroshima University

Principal Investigator

WASAKI Jun  広島大学, 生物圏科学研究科, 准教授 (00374728)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywordsリン / 有機酸 / 酸性ホスファターゼ / 分泌 / シロバナルーピン / トランスポーター / 土壌未利用リン / シロイヌナズナ
Outline of Final Research Achievements

It is expected to save amount of P fertilizer usage, because the resource is depleting. On the other hand, unavailable forms of P are accumulated in cultivated lands. Specific group of plants has ability to access the unavailable P by high ability to secrete organic acids and phosphatase. This study aimed to investigate and apply their functions.
Several genes for organic acid transporter have been isolated from plants, which are able to access unavailable P. It was suggested that these transporters contributed to higher ability of organic acid exudation. It was indicated that transformation of organic acid transporter and acid phosphatase improved the availability of P in the rhizosphere soil by their mobilizing functions.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (55 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results,  Acknowledgement Compliant: 2 results) Presentation (36 results) (of which Invited: 1 results) Book (1 results) Remarks (4 results)

  • [Journal Article] 日本産ヤマモガシ(Helicia cochinchinensis Lour.,ヤマモガシ科)のクラスター根の発見.2015

    • Author(s)
      山内 大輝
    • Journal Title

      植物研究雑誌

      Volume: 90 Pages: 103-108

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Interspecific facilitation of P acquisition in the intercropping of maize with white lupin in two contrasting soils as influenced by different rates and forms of P supply.2015

    • Author(s)
      Dissanayaka, D.M.S.B.
    • Journal Title

      Plant and Soil

      Volume: 390 Issue: 1-2 Pages: 223-236

    • DOI

      10.1007/s11104-015-2392-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 植物栄養学を生かした農業生産技術について. 3.リン酸栄養について-植物における難利用性リンの有効利用法-2015

    • Author(s)
      和崎 淳
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リン循環に関わる生物の機能に迫る分子生物学的手法2015

    • Author(s)
      和崎 淳
    • Journal Title

      地球環境

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phytate Degradation by Fungi and Bacteria that Inhabit Sawdust and Coffee Residue Composts2013

    • Author(s)
      Eida, M.F.
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 1 Pages: 71-80

    • DOI

      10.1264/jsme2.ME12083

    • NAID

      10031138546

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物による難利用性リン酸利用のしくみと活用の可能性.2013

    • Author(s)
      和崎 淳
    • Journal Title

      土づくりとエコ農業

      Volume: 45 Pages: 10-15

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts2012

    • Author(s)
      Eida, M.F.
    • Journal Title

      Microbes and Environments

      Volume: 27 Issue: 3 Pages: 226-233

    • DOI

      10.1264/jsme2.ME11299

    • NAID

      10030869067

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リン酸資源の枯渇に対応したリン栄養研究. 1.講座のねらい.2012

    • Author(s)
      俵谷 圭太郎
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 173-176

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リン酸資源の枯渇に対応したリン栄養研究. 2.有機態リン酸の利用.2012

    • Author(s)
      和崎 淳
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 177-182

    • NAID

      110009593697

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物の養分獲得・利用と施肥.2012

    • Author(s)
      瀬戸 二郎
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 471-477

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of exogenous phosphatase and phytase activities on organic phosphate mobilization in soils with different phosphate adsorption capacities2012

    • Author(s)
      Maruyama, H., Yamamura, T., Kaneko, Y., Matsui, H., Watanabe, T., Shinano, T., Osaki, M., Wasaki, J.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 58 Issue: 1 Pages: 41-51

    • DOI

      10.1080/00380768.2012.656298

    • NAID

      110009594821

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of secreted proteins from aseptically grown rice2011

    • Author(s)
      Shinano, T.
    • Journal Title

      Phytochemistry

      Volume: 72(4-5) Issue: 4-5 Pages: 312-320

    • DOI

      10.1016/j.phytochem.2010.12.006

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Compost amendment enhances population and composition of phosphate solubilizing bacteria and improves phosphorus availability in granitic regosols2011

    • Author(s)
      Wickramatilake, A. R. P., Munehiro, R., Nagaoka, T., Wasaki, J., Kouno, K.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 57 Issue: 4 Pages: 529-540

    • DOI

      10.1080/00380768.2011.600243

    • NAID

      110009327340

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of Cellulolytic and Hemicellulolytic Abilities of Fungi Isolated from Coffee Residue and Sawdust Composts2011

    • Author(s)
      Eida, M.F.
    • Journal Title

      Microbes and Environments

      Volume: 26 Issue: 3 Pages: 220-227

    • DOI

      10.1264/jsme2.ME10210

    • NAID

      10029653542

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Growth condition and P mobilizing properties of cluster roots of Helicia cochinchinensis (Proteaceae) at Miyajima Island, Japan.2015

    • Author(s)
      Wasaki, J.
    • Organizer
      Rhizosphere 4
    • Place of Presentation
      Maastricht, オランダ
    • Year and Date
      2015-06-21 – 2015-06-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 中国地方の貧栄養な花崗岩質土壌に生育する木本・草本植物におけるリン利用2014

    • Author(s)
      和崎 淳
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamics of phosphorus fractions in the rhizosphere of maize in intercropping cultivation system with white lupin as influenced by heterogeneous phosphorus rates and forms.2014

    • Author(s)
      D.M.S.B. Dissanayaka
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Physiological responses of Hakea laurina (Proteaceae) to low-P and high-P conditions.2014

    • Author(s)
      Maruyama, H.
    • Organizer
      5th International Symposium on Phosphorus Dynamics in the Soil-Plant Continuum
    • Place of Presentation
      Montpellier, フランス
    • Year and Date
      2014-08-26 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] De novo RNA-seqとCE-TOF/MSによる代謝産物プロファイリングに基づくシロバナルーピンの低リン耐性機構の解析2014

    • Author(s)
      丸山 隼人
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 日本産ヤマモガシで見つかった特異な根2014

    • Author(s)
      坪田 博美
    • Organizer
      日本植物分類学会第13回大会
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 環境サンプル測定のためのリン酸マイクロセンサーの開発2014

    • Author(s)
      谷内 翔
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effect of intercropped white lupin on growth and P uptake of maize under different levels and types of phosphorus application.2013

    • Author(s)
      Dissanayaka, D.M.S.B.
    • Organizer
      日本土壌肥料学会2013年度名古屋大会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヤマモガシ科植物Hakea laurinaの低リン耐性機構に関わる遺伝子の解析2013

    • Author(s)
      丸山 隼人
    • Organizer
      日本土壌肥料学会2013年度名古屋大会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] AtALMT3 is a malate transporter induced in roots of phosphorus deficient Arabidopsis thaliana.2013

    • Author(s)
      Maruyama, H.
    • Organizer
      International Workshop on Plant Membrane Biology XVI
    • Place of Presentation
      岡山県倉敷市
    • Related Report
      2012 Annual Research Report
  • [Presentation] リン欠乏及びアルミニウム障害時におけるシロイヌナズナ野生株およびリンゴ酸分泌変異株の根浸出物の解析2012

    • Author(s)
      丸山隼人, 他4名
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヤマモガシ科植物Hakea laurinaの低リン耐性における酸性ホスファターゼホモログの役割2012

    • Author(s)
      和崎淳、丸山隼人
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロバナルーピンを混植した主作物の生育とリン吸収の種間比較.2012

    • Author(s)
      和崎 淳
    • Organizer
      日本土壌肥料学会鳥取大会
    • Place of Presentation
      鳥取市
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヤマモガシ科植物Hakea laurinaの低リン耐性機構の解析.2012

    • Author(s)
      丸山 隼人
    • Organizer
      日本土壌肥料学会鳥取大会
    • Place of Presentation
      鳥取市
    • Related Report
      2012 Annual Research Report
  • [Presentation] ルーピンの根圏における細菌群集と有機態リンの動態に及ぼす有機酸の影響.2012

    • Author(s)
      松崎 弘佑
    • Organizer
      日本土壌肥料学会鳥取大会
    • Place of Presentation
      鳥取市
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロバナルーピンのクラスター根から単離したALMTホモログの解析.2012

    • Author(s)
      矢倉 興士
    • Organizer
      日本土壌肥料学会鳥取大会
    • Place of Presentation
      鳥取市
    • Related Report
      2012 Annual Research Report
  • [Presentation] リン溶解菌による土壌蓄積難溶性無機リンの利用性改善. ―有機物中のリン溶解菌の単離及び難溶性無機リン溶解能力の評価―.2012

    • Author(s)
      宗廣 亮佑
    • Organizer
      日本土壌肥料学会鳥取大会
    • Place of Presentation
      鳥取市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Comparative transcriptomic analysis of P deficient plant roots.2012

    • Author(s)
      Wasaki, J.
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture IV
    • Place of Presentation
      パース(オーストラリア)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Physiological responses of Hakea laurina (Proteaceae) to low-P conditions.2012

    • Author(s)
      Maruyama, H.
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture IV
    • Place of Presentation
      パース(オーストラリア)
    • Related Report
      2012 Annual Research Report
  • [Presentation] “Dauciform roots” of Japanese native Cypereceae (sedge) and involvement of phosphorus status.2012

    • Author(s)
      Masuda, G.
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture IV
    • Place of Presentation
      パース(オーストラリア)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 非菌根性シロバナルーピンの根圏におけるリン吸収能力とその活用2011

    • Author(s)
      和崎淳
    • Organizer
      菌根研究会特別講演
    • Place of Presentation
      広島県東広島市(招待講演)
    • Year and Date
      2011-12-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phosphorus starvation changes the metabolic flow in rice2011

    • Author(s)
      Shinano, T., et al
    • Organizer
      3rd International Symposium Frontiers in Agriculture Proteome Research-Contribution of proteomics technology in agricultural sciences-
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-11-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物機能の活用による土壌蓄積リンの効率的利用技術2011

    • Author(s)
      和崎淳
    • Organizer
      関東東海土壌肥料部会・秋季研究会
    • Place of Presentation
      埼玉県熊谷市(招待講演)
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of malate and citrate transporters induced in roots of phosphorus deficient Arabidopsis thaliana2011

    • Author(s)
      Maruyama, H., et al
    • Organizer
      Rhizosphere 3 International Conference
    • Place of Presentation
      Perth (Australia)
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of phosphate utilization in Arabidopsis thaliana natural accessions grown on soil2011

    • Author(s)
      Furutani, A., et al
    • Organizer
      Rhizosphere 3 International Conference
    • Place of Presentation
      Perth (Australia)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Interactions between plants and phytate mineralizing bacteria isolated from the rhizosphere2011

    • Author(s)
      Mizukami, N., Wasaki, J.
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌市
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物の養分ホメオスタシス(2)リン酸2011

    • Author(s)
      和崎淳
    • Organizer
      日本土壌肥料学会シンポジウム
    • Place of Presentation
      茨城県つくば市(招待講演)
    • Year and Date
      2011-08-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 異なる土壌で栽培したトウモロコシのリン吸収に及ぼすシロバナルーピン混植の効果2011

    • Author(s)
      上脇葉子, 他4名
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] リン欠乏シロイヌナズナ根からのリンゴ酸およびクエン酸分泌に関わるトランスポーターの解析2011

    • Author(s)
      丸山隼人, 他3名
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] マサ土における土壌蓄積リンの植物による利用性と微生物性との関連2011

    • Author(s)
      宗廣亮佑, 他4名
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Evaluation of cellulolytic and hemicellulolytic abilities of bacteria isolated from coffee residue and sawdust composts2011

    • Author(s)
      Mohamed Fathallah Eida, 他3名
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of malate transporters induced in roots of phosphorus deficient oraluminum stressed Arabidopsis thaliana2011

    • Author(s)
      Maruyama, H., et al
    • Organizer
      International Conference on Arabidopsis Research 2011
    • Place of Presentation
      Madison, WI (USA)
    • Year and Date
      2011-06-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ナキリスゲ(Carex lenta)のダウシフォーム根形成に与える土壌リンの影響

    • Author(s)
      枡田 元気
    • Organizer
      第61回日本生態学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 日本在来のヤマモガシ科ヤマモガシから奇妙な形の「クラスター根」を発見した

    • Author(s)
      山内 大輝
    • Organizer
      第61回日本生態学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 難利用性リンの積極的獲得に関わる根分泌物質の輸送と制御.

    • Author(s)
      和崎 淳
    • Organizer
      岡山大学資源植物科学研究所 拠点共同研究主催 ワークショップ「植物ホルモンとイオン輸送体の相互理解」
    • Place of Presentation
      岡山県倉敷市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] シロバナルーピンの混植がトウモロコシのリン吸収に及ぼす影響.

    • Author(s)
      入江 智史
    • Organizer
      日本土壌肥料学会関西支部講演会
    • Place of Presentation
      岡山県倉敷市
    • Related Report
      2012 Annual Research Report
  • [Book] Principles, Application and Assessment in Soil Science2011

    • Author(s)
      Watanabe, T., et al
    • Total Pages
      394
    • Publisher
      InTech
    • Related Report
      2011 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://home.hiroshima-u.ac.jp/rhizo/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://home.hiroshima-u.ac.jp/rhizo/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://home.hiroshima-u.ac.jp/rhizo/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/rhizo/

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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