| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
Two-component system (TCS) is a predominant mode of signal transduction in bacteria. Recent progresses have been drawing detailed pictures of their regulatory networks and evolutional traces. Yet, systematic experimental analyses across species and their statistics are quite challenging. Here, we aimed to analyze cross-regulatory profiles in TCSs of Salmonella enterica and its closely related bacteria by constructing approximately 30 TCS mutant strains for each species. In order to detect novel cross-regulatory phenomena in a quantitative manner, a representative gene promoter, which is directly regulated by a given TCS, was selected for each TCS and cloned into a promoter-less luciferase (lux) reporter/ broad range conjugation plasmid. By mating and testing epistasis in TCSs, we compare extended “TCSs cross-regulatory profiling maps” across species in details. From a certain aspect of these relating analyses, novel TCS regulatory mechanisms were established lately.
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