Study on the molecular basis underlyng neurite outgrowth promotion by gangliosides
Project/Area Number |
23688014
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Bioproduction chemistry/Bioorganic chemistry
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Research Institution | Gifu University |
Principal Investigator |
ANDO Hiromune 岐阜大学, 応用生物科学部, 准教授 (20372518)
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Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Kenichi 京都大学, 物質-細胞統合システム拠点, 准教授 (50423059)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
|
Keywords | ガングリオシド / 神経突起伸展 / 構造活性相関 / 一分子観察 / 神経突起伸展活性 / PC12細胞 / 糖鎖 / シアル酸 / PC12 / 棘皮度物 / 糖脂質 / 糖鎖合成化学 |
Outline of Final Research Achievements |
The goal of this research is to clarify the molecular basis underlyning the neurite outgrowth promotion by exogenous gangliosides. We have succeeded in the first total syntheses of neuritogenic gangliosides and their analogues based on the newly developed synthetic method. The synthesized gangliosides were utilized for neurite outgrowth evaluation, by which the structure responsible for the activity was revealed for the first time. Furthermore, we have also developed novel fluorescently labeled ganglioside probes suitable for observing the behavior of ganglioside in living cell membrane. The single molecular observation using the ganglioside probes allowed to observe how ganglioside molecule moves in the cell membrane (done by collaboration with Prof. Kenichi Suzuki of Kyoto University).
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Report
(5 results)
Research Products
(72 results)
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[Journal Article] Surface Functionalization of Protein Crystals with Carbohydrate Using Site-selective Bioconjugation2015
Author(s)
S. Abe, Y. Tokura, R. Pal, N. Komura, A. Imamura, K. Matsumoto, H. Ijiri, N. J. M. Sanghamitra, H. Tabe, H. Ando, M. Kiso, H. Mori, S. Kitagawa, and T. Ueno
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Journal Title
Chem. Lett.
Volume: 44
Pages: 29-31
DOI
Related Report
Peer Reviewed
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[Journal Article] Interaction of Platelet Endothelial Cell Adhesion Molecule (PECAM) with α2,6-Sialylated Glycan Regulates Its Cell Surface Residency and Anti-apoptotic Role2014
Author(s)
S. Kitazume, R. Imamaki, A. Kurimoto, K. Ogawa, M. Kato, Y. Yamaguchi, K. Tanaka, H. Ishida, H. Ando, M. Kiso, N. Hashii, N. Kawasaki, N. Taniguchi
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Journal Title
J. Biol.Chem.
Volume: 289
Pages: 27604-27613
DOI
Related Report
Peer Reviewed
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[Journal Article] Expanded potential of seleno-carbohydrates as a molecular tool for X-ray structural determination of a carbohydrate-protein complex with single/multiwavelength anomalous dispersion phasing2014
Author(s)
T. Suzuki, H. Makyio, H. Ando, N. Komura, M. Menjo, Y. Yamada, A. Imamura, H. Ishida, S. Wakatsuki, R. Kato, M. Kiso
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Journal Title
Bioorg. Med. Chem.
Volume: 22
Pages: 2090-2101
DOI
Related Report
Peer Reviewed
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[Journal Article] The first total synthesis of ganglioside GalNAc-GD 1a, a target molecule for autoantibodies in Guillain-Barre syndrome2011
Author(s)
K.Fujikawa, S.Nakashima, M.Konishi, T.Fuse, N.Komura, T.Ando, H.Ando, N.Yuki, H.Ishida, M.Kiso
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Journal Title
Chemistry A European Journal
Volume: 17
Pages: 5641-5651
DOI
Related Report
Peer Reviewed
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[Presentation] ynthetic study on neuritegenic glycophospholipid CJP-series from comatulida Comanthus japonica2014
Author(s)
Tamai, H., Takeda, Y., Takashi, M., Ando, H., Ueki, A., Imamura, A., Ishida, H., Kiso, M.
Organizer
Sialoglyco2014
Place of Presentation
Gold Coast, Australia
Year and Date
2014-09-06 – 2014-09-12
Related Report
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[Presentation] Development of a-series gangliosides probes for imaging of raft-associated signaling.2013
Author(s)
Komura, N., Suzuki, K., Ando, H., Ishida, H., Kusumi, A., Kiso, M.
Organizer
The 5th Asian Communications of Glycobiology and Glycotechnology
Place of Presentation
Khon Kaen, Thailand
Year and Date
20131014-20131018
Related Report
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[Presentation] Synthesis and functional evaluation of fluorescent ganglioside GD1b useful for live imaging of cell membrane.2013
Author(s)
Konishi, M., Suzuki, K., Imamura, A., Ando, H., Ishida, H., Kusumi, A., Kiso, M
Organizer
The 5th Asian Communications of Glycobiology and Glycotechnology
Place of Presentation
Khon Kaen, Thailand
Year and Date
20131014-20131018
Related Report
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[Presentation] Novel fluorescent glycolipid probe for single molecule imaging of mesoscale domain in living cell membrane2013
Author(s)
N. Komura,K. G. N. Suzuki, H. Ando, R. Chadda, H. Tsuboi, T. Ikeda, K. Tanaka, H. Ishida, A. Kusumi, M. Kiso
Organizer
Challenges in Organic Materials & Supramolecular Chemistry (ISACS10)
Place of Presentation
Kyoto, Japan
Year and Date
20130718-20130721
Related Report
Invited
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[Presentation] Development of ganglioside probes as raft markers2011
Author(s)
Ando, H., Komura, N., Chadda, R., Tsuboi, H., Ikeda, T., Tanaka, K., Suzuki, K., Kusumi, A., Ishida, H., Kiso, M
Organizer
第71回岡崎コンファレンス~糖鎖分子科学の新たな展望~
Place of Presentation
愛知
Year and Date
20111012-20111014
Related Report
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[Presentation] Development of novel fluorescent ganglioside analogs as raft markers2011
Author(s)
Komura, N., Ando, H., Matsui, I., Tanaka, K., Suzuki, K., Kusumi, A., Ishida, H., Kiso, M
Organizer
16th European Carbohydrate Symposium
Place of Presentation
Sorrento-Naples, Italy
Year and Date
20110703-20110707
Related Report
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