The effect of starvation on fish survival against harmful algal bloom and diseases

• Project/Area Number: 23688022
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Ehime University
• Principal Investigator: OHTA KOHEI 愛媛大学, 南予水産研究センター, 准教授 (10585764)
• Co-Investigator(Renkei-kenkyūsha): MATSUBARA Takahiro 愛媛大学, 南予水産研究センター, 教授 (60443389)
• Research Collaborator: URASAKI Shintarou 愛媛大学, 南予水産研究センター, 客員研究員・愛南町 ; SHIMIZU Sonoko 愛媛大学, 南予水産研究センター, 准教授 ; MOHAPATRA Sipra 愛媛大学, 南予水産研究センター, 博士研究員 ; CHAKRABORTY Tapas 愛媛大学, 南予水産研究センター, 博士研究員 ; KAMADA Kousuke 愛媛大学, 農学部・海洋生産科学特別コース ; MUKAI Kouki 愛媛大学, 農学部・海洋生産科学特別コース ; TANAKA Shunya 愛媛大学, 農学部・海洋生産科学特別コース ; KITAGAWA Yuuya 愛媛大学, 農学部・海洋生産科学特別コース
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000); Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 絶食効果 / 魚類生理 / 水産養殖 / 赤潮対策 / 魚病対策

Outline of Final Research Achievements

For the sustainable fish farming, harmful algal bloom (HAB) and diseases are serious problems. In the cases of HAB and diseases, fasting is the common technique to prevent the damage of fish. However, the effect of starvation on fish survival is largely unknown. To understand and improve the fasting technique, we studied the physiological and molecular roles of starvation. When fish were starved, decreases in some amino acids levels were observed in addition to serum glucose levels and oxygen consumption rates. On the other hand, starvation induced better immune and metabolic conditions than the non-starved fish after bacterial infection. At the exposure to HAB, acidosis occurred due to the oxygen deficiency, suggesting that the decrease of oxygen consumption in the starved fish leads to higher survivability. Additionally, it was suggested that the decreases of some nutrients enhance the survival through the other mechanisms.

Research Products

• [Journal Article] Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge (2018)
  • Author(s): Noman Reza Mohammad Ali、Mohapatra Sipra、Shimizu Sonoko、Kitamura Shin-Ichi、Harakawa Shogo、Kawakami Hidemasa、Nakayama Kei、Sawayama Eitaro、Matsubara Takahiro、Ohta Kohei、Chakraborty Tapas
  • Journal Title: Fish & Shellfish Immunology Volume: 82 Pages: 286-295
  • DOI: 10.1016/j.fsi.2018.08.027
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 赤潮対策として用いられる餌止め効果の科学的メカニズム養殖技術講座-赤潮対策- (2018)
  • Author(s): 太田耕平
  • Journal Title: 月刊「養殖ビジネス」 Volume: 3月号 Pages: 51-56
• [Journal Article] Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision (2017)
  • Author(s): Chakraborty Tapas、Mohapatra Sipra、Tobayama Megumi、Ohta Kayoko、Ryu Yong-Woon、Kazeto Yukinori、Ohta Kohei、Zhou Linyan、Nagahama Yoshitaka、Matsubara Takahiro
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 3183-3183
  • DOI: 10.1038/s41598-017-03314-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Short-term starvation and realimentation helps stave off Edwardsiella tarda infection in red sea bream (Pagrus major) (2017)
  • Author(s): Mohapatra Sipra、Chakraborty Tapas、Reza Mohammad Ali Noman、Shimizu Sonoko、Matsubara Takahiro、Ohta Kohei
  • Journal Title: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Volume: 206 Pages: 42-53
  • DOI: 10.1016/j.cbpb.2017.01.009
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Starvation : An Alternate Measure to Improve Immunity and Physiology of Red Sea Bream During Edwardsiella Tarda Infection (2016)
  • Author(s): Sipra Mohapatra, Tapas Chakraborty, Rami Haj-Kacem, Sonoko Shimizu, Takahiro Matsubara, Kohei Ohta
  • Journal Title: Journal of Aquaculture Research & Development Volume: S2 Pages: 007-007
  • DOI: 10.4172/2155-9546.s2-007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Starvation beneficially influences the liver physiology and nutrient metabolism in Edwardsiella tarda infected red sea bream (Pagrus major) (2015)
  • Author(s): Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Shintaro Urasaki, Takahiro Matsubara, Yoshitaka Nagahama, Kohei Ohta
  • Journal Title: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology Volume: 189 Pages: 1-10
  • DOI: 10.1016/j.cbpa.2015.07.003
  • Peer Reviewed
• [Journal Article] Bidirectional sex change induced by sex steroid implantation in the hermaphrodite fish, Pseudolabrus sieboldi. (2012)
  • Author(s): Ohta, K., Sakai, M., Sundaray, J.K., Kitano, T., Takeda, T., Yamaguchi, A., Matsuyama, M.
  • Journal Title: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology Volume: 317 Issue: 9 Pages: 552-560
  • DOI: 10.1002/jez.1747
  • Peer Reviewed
• [Journal Article] Male differentiation of germ cells induced by embryonic-age-specific Sertoli cells in mice (2012)
  • Author(s): Ohta, K., Yamamoto, M., Lin, Y., Hogg, N., Akiyama, H., Behringer, RR., Yamazaki, Y.
  • Journal Title: Biology of Reproduction Volume: 86 Issue: 4 Pages: 1-11
  • DOI: 10.1095/biolreprod.111.095943
  • Peer Reviewed
• [Journal Article] Molecular cloning of two gonadotropin receptors and their distinct mRNA expression profiles in daily oogenesis of the wrasse Pseudolabrus sieboldi (2011)
  • Author(s): Kitano, H., Me, S., Ohta, K., Hirai, T., Yamaguchi, A., Matsuyama, M.
  • Journal Title: General and Comparative Endocrinology Volume: 172 Issue: 2 Pages: 268-276
  • DOI: 10.1016/j.ygcen.2011.03.012
  • Peer Reviewed
• [Presentation] 養殖海域における有害プランクトン４種の高感度モニタリング (2013)
  • Author(s): 清水園子, 浦崎慎太郎, 松原孝博，太田耕平
  • Organizer: 平成２４年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] カツオ一本釣用カタクチイワシ（Engraulis japonica）養殖基盤のための成長と変態特性の解明 (2013)
  • Author(s): 柳蓉沄・田中真誠・石田大二・後藤理恵・太田耕平・松原孝博
  • Organizer: 平成２４年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] 愛南町海域環境調査について (2012)
  • Author(s): 太田耕平
  • Organizer: 第2回愛南町水産フォーラム
  • Place of Presentation: 愛媛県愛南町
  • Year and Date: 2012-02-18
• [Presentation] Research on the detoxification ability of annelids for bioremediation in coastal benthic environment. (2012)
  • Author(s): Ito K., Ito M., Onduka T., Ohta K., Hano T., Mochida K., Ohkubo N., Miura T., Fujii K,.
  • Organizer: Forum on Fishery Science and Technology.
  • Place of Presentation: Shanghai, China.
• [Presentation] The difference of bioremediation ability among four annelids species inhabiting the polluted area. (2012)
  • Author(s): Ito K., Ito M., Onduka T., Ohta K., Hano T., Mochida K., Miura T., Fujii K,.
  • Organizer: SEATAC Asia/Pacific.
  • Place of Presentation: 熊本
• [Presentation] Male differentiation of germ cells induced by embryonic-age-specific Sertoli cells in mice (2011)
  • Author(s): 太田耕平, 浦崎慎太郎, 清水園子, 井戸篤史, 三浦智恵美, 松原孝博, 三浦猛
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎
  • Year and Date: 2011-10-01
• [Presentation] ホシササノハベラ生殖腺の性転換に伴うステロイド代謝酵素および性分化関連因子の遺伝子発現変動 (2011)
  • Author(s): 太田耕平, 松山倫也, 長濱嘉孝
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎
  • Year and Date: 2011-09-29
• [Presentation] マウス胎仔期セルトリ細胞は時期特異的に始原生殖細胞(PGCs)を雄性化する (2011)
  • Author(s): 太田耕平, 山本みゆき, Yanling Lin, Nathanael Hogg, 秋山治彦, Richard R.Behringer, 山崎由起子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-22
• [Patent(Industrial Property Rights)] 赤潮検出用プライマーセット、及び赤潮検出方法. (2014)
  • Inventor(s): 太田耕平、清水園子、松原孝博、浦﨑慎太郎
  • Industrial Property Rights Holder: 太田耕平、清水園子、松原孝博、浦﨑慎太郎
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-02-05