Elucidation of physical function of an odorant-binding protein found in bovine milk

• Project/Area Number: 23688032
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUDA Kenji 帯広畜産大学, 畜産学部, 准教授 (80419217)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000); Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 運搬タンパク質 / リポカリン / フェロモン / ミルク / odorant-binding protein / 匂い物質 / アフィニティー精製 / GC-MS / bis-ANS / モノクローナル抗体 / ウエスタンブロッティング / リアルタイムPCR / CxxxCモチーフ

Research Abstract

Bovine colostral odorant-binding protein (bcOBP), found in bovine colostrum, is a transport protein belonging to lipocalin superfamily. bcOBP was present not only in milk but also in saliva, nasal mucus, vaginal discharge, and blood plasma. Its mRNA was widely expressed in several tissues. bcOBP was highly specific in terms of ligand recognition.

Research Products

• [Journal Article] Cloning, monoclonal antibody production, and bodily distribution pattern of a bovine lipocalin (2012)
  • Author(s): Tamar Japaridze, Akitsugu Senda, Hirofumi Nozaki, Mayumi Yanagida, Takumi Suzuki, Khuukhenbaatar Ganzorig, Yasunori Kushi, Katsuya Kida, Tadasu Urashima, Rupert M. Bruckmaier, Kenji Fukuda
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76巻 Pages: 712-720
  • NAID: 10030751590
  • Peer Reviewed
• [Journal Article] Cloning, Monoclonal Antibody Production, and Bodily Distribution Pattern of a Bovine Lipocalin (2012)
  • Author(s): Japaridze T, Senda A, Nozaki H, Yanagida M, Suzuki T, Ganzorig K, Kushi Y, Kida K, Urashima T, Bruckmaier RM, Fukuda K
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 4 Pages: 712-720
  • DOI: 10.1271/bbb.110840
  • NAID: 10030751590
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] ミルクに含まれる運搬タンパク質 (2013)
  • Author(s): 福田健二
  • Organizer: 2013年度酪農科学シンポジウム
  • Place of Presentation: 岡山大学創立五十周年記念館（岡山市)
• [Presentation] Carrier proteins in milk: basic and potential applications (2012)
  • Author(s): Kenji Fukuda
  • Organizer: The 2nd International Seminar on Animal Industry 2012
  • Year and Date: 2012-07-05
• [Presentation] ウシリポカリンの生体内分布 (2012)
  • Author(s): Tamar Japaridze,福田 健二,片山 翔太,木田 克弥, Rupert Bruckmaier,浦島 匡
  • Organizer: 日本農芸化学会2011年度大会
  • Year and Date: 2012-03-25
• [Presentation] ウシリポカリンの生体内分布 (2012)
  • Author(s): Tamar Japaridze、福田健二、片山翔太、木田克弥、Rupert Bruckmaier、浦島匡
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学(京都)
  • Year and Date: 2012-03-25
• [Presentation] ウシ初乳由来odorant-binding protein の生体内分布 (2011)
  • Author(s): 福田健二, Tamar Japaridze,高松真理佳,片山翔太,浦島 匡
  • Organizer: 日本酪農科学会2011年度シンポジウム
  • Year and Date: 2011-09-22
• [Presentation] ウシ初乳由来odorant-binding proteinの生体内分布 (2011)
  • Author(s): 福田健二、Tamar Japaridze、高松真理佳、片山翔太、浦島匡
  • Organizer: 日本酪農科学会
  • Place of Presentation: フォレスト仙台(仙台)
  • Year and Date: 2011-09-22
• [Presentation] Expression Profile of Bovine Colostral Odorant Binding Protein in Various Biological Fluids of Dairy Cow (2011)
  • Author(s): Tamar Japaridze, Takumi Suzuki, Katsuya Kida, Rupert Bruckmaier, Tadasu Urashima, and Kenji Fukuda
  • Organizer: 7th International Conference on Farm Animal Endocrinology
  • Year and Date: 2011-08-25
• [Presentation] Expression Profile of Bovine Colostral Odorant Binding Protein in Various Biological Fluids of Dairy Cow (2011)
  • Author(s): Tamar Japaridze, Takumi Suzuki, Katsuya Kida, Rupert Bruckmaier, Tadasu Urashima, Kenji Fukuda
  • Organizer: 7th International Conference on Farm Animal Endocrinology
  • Place of Presentation: Bern University (Bern)
  • Year and Date: 2011-08-25
• [Remarks]
  • URL: http://ir.obihiro.ac.jp/