Understanding of the pheromonal control of emotions

• Project/Area Number: 23688035
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: The University of Tokyo
• Principal Investigator: KIYOKAWA Yasushi 東京大学, 農学生命科学研究科, 助教 (70554484)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 警報フェロモン / 安寧フェロモン / 社会的緩衝作用 / 揮発性分子 / 鋤鼻系 / 主嗅覚系 / 分界条床核 / 扁桃体 / フェロモン / 不安 / 恐怖 / 社会的ストレス緩衝作用 / 聴覚性驚愕反射 / Tenax / 恐怖条件づけ / すくみ行動 / 聴覚性驚愕反応

Outline of Final Research Achievements

Supported by this grant, we found that rats released 4-methylpentanal and hexanal from the perianal region when they were stressed. These molecules activated the bed nucleus of the stria terminalis and evoked a variety of anxiety responses in other rats when rats detected these molecules simultaneously. We also found that the volatile appeasing pheromone released from non-stressed rats suppressed the amygdala activation to the auditory conditioned stimuli, which resulted in the amelioration of stress responses.

Research Products

• [Journal Article] Neural correlates underlying naloxone-induced amelioration of sexual behavior deterioration due to an alarm pheromone (2015)
  • Author(s): Kobayashi, T., Kiyokawa, Y., Takeuchi, Y. and Mori, Y.
  • Journal Title: Frontiers in Neuroscience Volume: 9 Pages: 52-52
  • DOI: 10.3389/fnins.2015.00052
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Social buffering suppresses fear-associated activation of the lateral amygdala in male rats: behavioral and neurophysiological evidence (2015)
  • Author(s): Fuzzo, F., Matsumoto, J., Kiyokawa, Y., Takeuchi, Y., Ono, T. and Nishijo, H.
  • Journal Title: Frontiers in Neuroscience Volume: 9 Pages: 99-99
  • DOI: 10.3389/fnins.2015.00099
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A familiar conspecific is more effective than an unfamiliar conspecific for social buffering of conditioned fear responses in male rats (2014)
  • Author(s): Kiyokawa Y., Honda A., Takeuchi Y., Mori Y.
  • Journal Title: Behav Brain Res Volume: 267 Pages: 189-193
  • DOI: 10.1016/j.bbr.2014.03.043
  • Peer Reviewed
• [Journal Article] Identification of a pheromone that increases anxiety in rats (2014)
  • Author(s): Inagaki H., Kiyokawa Y., Tamogami S., Watanabe H., Takeuchi Y., Mori Y.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111 Issue: 52 Pages: 18751-18756
  • DOI: 10.1073/pnas.1414710112
  • Peer Reviewed / Open Access
• [Journal Article] Social buffering reduces male rats' behavioral and corticosterone responses to a conditioned stimulus (2014)
  • Author(s): Kiyokawa Y., Hiroshima S., Takeuchi Y., Mori Y.
  • Journal Title: Horm Behav Volume: 65 Issue: 2 Pages: 114-118
  • DOI: 10.1016/j.yhbeh.2013.12.005
  • Peer Reviewed
• [Journal Article] Naloxone Blocks the Deterioration of Male Sexual Behavior Induced by Alarm Pheromone in Rats (2013)
  • Author(s): Kobayashi T., Kiyokawa Y., Arata S., Takeuchi Y., Mori Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 6 Pages: 761-765
  • DOI: 10.1292/jvms.12-0454
  • NAID: 130003362126
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Alarm pheromone is detected by the vomeronasal organ in male rats (2013)
  • Author(s): Kiyokawa Y., Kodama Y., Kubota T., Takeuchi Y., Mori Y.
  • Journal Title: Chem Senses Volume: 38 Issue: 8 Pages: 661-668
  • DOI: 10.1093/chemse/bjt030
  • Peer Reviewed
• [Journal Article] Physical interaction is not necessary for the induction of housing-type social buffering of conditioned hyperthermia in male rats (2013)
  • Author(s): Kiyokawa, Y., Kodama, Y., Takeuchi, Y. and Mori, Y.
  • Journal Title: Behavioural Brain Research Volume: 256 Pages: 414-419
  • DOI: 10.1016/j.bbr.2013.08.037
  • Peer Reviewed
• [Journal Article] C-Fos expression during the modulation of sexual behavior by an alarm pheromone (2013)
  • Author(s): Kobayashi, T., Kiyokawa, Y., Arata, S., Takeuchi, Y. and Mori, Y.
  • Journal Title: Behavioural Brain Research Volume: 237 Pages: 230-237
  • DOI: 10.1016/j.bbr.2012.09.042
  • Peer Reviewed
• [Journal Article] Olfactory signals mediate social buffering of conditioned fear responses in male rats (2013)
  • Author(s): Takahashi Y., Kiyokawa Y., Kodama Y., Arata S., Takeuchi Y., Mori Y.
  • Journal Title: Behav Brain Res Volume: 240 Pages: 46-51
  • DOI: 10.1016/j.bbr.2012.11.017
  • Peer Reviewed
• [Journal Article] The neural pathway underlying social buffering of conditioned fear responses in male rats. (2012)
  • Author(s): Kiyokawa Y, Wakabayashi Y, Takeuchi Y, Mori Y.
  • Journal Title: Eur J Neurosci Volume: 36 Issue: 10 Pages: 3429-3437
  • DOI: 10.1111/j.1460-9568.2012.08257.x
  • Peer Reviewed
• [Journal Article] Male Rats Respond to Their Own Alarm Pheromone (2012)
  • Author(s): Inagaki H, Kiyokawa Y, Takeuchi Y, Mori Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 1 Pages: 79-82
  • DOI: 10.1292/jvms.11-0225
  • NAID: 130001032839
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Pretreatment with CP-154526 blocks the modifying effects of alarm pheromone on components of sexual behavior in male, but not in female, rats. (2011)
  • Author(s): Kobayashi T, Kiyokawa Y, Takeuchi Y, Mori Y.
  • Journal Title: Chem Senses Volume: 36 Issue: 7 Pages: 623-632
  • DOI: 10.1093/chemse/bjr017
  • Peer Reviewed
• [Journal Article] 哺乳類の脳と匂い (2011)
  • Author(s): 清川泰志、武内ゆかり、森裕司
  • Journal Title: Aroma Research Volume: 12 Pages: 112-116
• [Presentation] Alarm pheromone-induced defensive behavior in rats: role of the bed nucleus of the stria terminalis (2015)
  • Author(s): Breitfeld, T., Bruning, J., Kiyokawa, Y., and Fendt, M.
  • Organizer: 11th Gottingen Meeting of the German Neuroscience Society
  • Place of Presentation: Gottingen, Germany
  • Year and Date: 2015-03-18
• [Presentation] Decreased fear-related evoked potential in rat's amygdala during social buffering (2014)
  • Author(s): Fuzzo, F., Matsumoto, J., Kiyokawa, Y., Mori, Y., Ono, T., and Nishijo, H.
  • Organizer: 第61回中部日本生理学会
  • Place of Presentation: 名古屋市立大学（愛知県）
  • Year and Date: 2014-11-07
• [Presentation] Effect of social buffering on fear extinction in male rats (2014)
  • Author(s): Mikami, K., Kiyokawa, Y., Takeuchi, Y. and Mori, Y.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kanagawa, Japan
  • Year and Date: 2014-09-12
• [Presentation] Auditory conditioned stimulus asymmetrically activates central amygdala in male rats (2014)
  • Author(s): Takahashi, D., Kiyokawa, Y., Takeuchi, Y. and Mori, Y.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kanagawa, Japan
  • Year and Date: 2014-09-11
• [Presentation] 性周期がストレス反応に与える影響の検討ー雌における社会的ストレス緩衝作用の研究に向けてー (2014)
  • Author(s): 石井晶子、清川泰志、武内ゆかり、森裕司
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（北海道）
  • Year and Date: 2014-09-09
• [Presentation] An alarm pheromone in rats (2014)
  • Author(s): Kiyokawa, Y., Inagaki, H., Takeuchi, Y. and Mori, Y.
  • Organizer: The 23rd Annual Meeting of the International Behavioral Neuroscience Society
  • Place of Presentation: Las Vegas, Nevada, USA
  • Year and Date: 2014-06-13
  • Invited
• [Presentation] Characterization of fear extinction using 3-sec auditory conditioned stimulus in male rats (2013)
  • Author(s): Mikami, K., Kiyokawa, Y., Takeuchi, Y. and Mori, Y.
  • Organizer: The 36th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] Social buffering as a novel way to suppress fear responses (2013)
  • Author(s): Kiyokawa, Y.
  • Organizer: The 36th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 国立京都国際会館（京都）
  • Invited
• [Presentation] A familiar conspecific is more effective in social buffering of conditioned fear responses in male rats (2013)
  • Author(s): Kiyokawa, Y., Honda, A., Takeuchi, Y. and Mori, Y.
  • Organizer: The 22nd Annual Meeting of the International Behavioral Neuroscience Society
  • Place of Presentation: Malahide, Ireland
• [Presentation] 社会的ストレス緩衝作用：社会的距離が与える影響 (2013)
  • Author(s): 清川泰志、本田章、武内ゆかり、森裕司
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜）
• [Presentation] フェロモンによる情動制御メカニズムの解明 (2013)
  • Author(s): 清川泰志、稲垣秀晃、武内ゆかり、森裕司
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] 社会的ストレス緩衝作用による恐怖反応の抑制 (2012)
  • Author(s): 清川泰志、武内ゆかり、森裕司
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 埼玉県
  • Year and Date: 2012-03-27
• [Presentation] 情動系に作用するフェロモン (2012)
  • Author(s): 清川泰志
  • Organizer: 平成23年度日本獣医師会学会年次大会(北海道)
  • Place of Presentation: 北海道
  • Year and Date: 2012-02-03
• [Presentation] Appeasing pheromone mediates social buffering of conditioned fear responses (2012)
  • Author(s): Kiyokawa, Y., Takahashi, Y., Takeuchi, Y. and Mori, Y.
  • Organizer: The 21th Annual Meeting of the International Behavioral Neuroscience Society
  • Place of Presentation: Hawaii, USA
• [Presentation] ラット警報フェロモンが超音波発声に与える影響 (2012)
  • Author(s): 六山寛美、稲垣秀晃、清川泰志、武内ゆかり、森裕司
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 盛岡
• [Presentation] Olfactory signals mediates social buffering of conditioned fear responses in male rats (2012)
  • Author(s): Kiyokawa, Y., Takahashi, Y., Takeuchi, Y. and Mori, Y.
  • Organizer: The 35th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Aichi, Japan
• [Presentation] Olfactory signals mediates social buffering of conditioned fear responses in male rats (2012)
  • Author(s): Kiyokawa, Y.
  • Organizer: The 10th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: Fukuoka, Japan
  • Invited
• [Presentation] The anterior olfactory nucleus is an important relay point connecting the main olfactory bulb and the amygdala in social buffering (2011)
  • Author(s): Kiyokawa, Y., Takeuchi, Y., Mori, Y.
  • Organizer: The 34th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-09-17
• [Presentation] Neural pathway for alarm-pheromone induced suppression of male sexual behavior in rats (2011)
  • Author(s): Kobayashi, T., Kiyokawa, Y., Takeuchi, Y., Mori, Y.
  • Organizer: The 34th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-09-16
• [Presentation] 警報フェロモンによる性行動の抑制に対するオピオイド受容体拮抗薬投与の影響 (2011)
  • Author(s): 小林辰也、清川泰志、武内ゆかり、森裕司
  • Organizer: 日本動物心理学会(第71回)・日本動物行動学会(第30回)・応用動物行動学会/日本家畜管理学会(2011年度)合同大会
  • Place of Presentation: 東京都
  • Year and Date: 2011-09-08
• [Presentation] Alarm pheromone suppresses sexual behavior in male rats (2011)
  • Author(s): Kobayashi, T., Kiyokawa, Y., Takeuchi, Y., Mori, Y.
  • Organizer: The 20th Annual Meeting of the International Behavioral Neuroscience Society
  • Place of Presentation: Colorado, USA
  • Year and Date: 2011-05-28
• [Presentation] Anterior olfactory nucleus suppresses ipsilateral amygdala in social buffering of conditioned fear responses (2011)
  • Author(s): Kiyokawa, Y., Takeuchi, Y., Mori, Y.
  • Organizer: The 20th Annual Meeting of the International Behavioral Neuroscience Society
  • Place of Presentation: Colorado, USA
  • Year and Date: 2011-05-28
• [Presentation] 社会的ストレス緩衝作用による恐怖反応の抑制 (2011)
  • Author(s): 清川泰志、武内ゆかり、森裕司
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2011-04-01
• [Presentation] 警報フェロモンによる性行動の抑制とオピオイド受容体拮抗薬投与の影響 (2011)
  • Author(s): 小林辰也、清川泰志、武内ゆかり、森裕司
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2011-04-01
• [Remarks] 獣医動物行動学研究室
  • URL: http://www.vm.a.u-tokyo.ac.jp/koudou/
• [Remarks] http://www.vm.a.u-tokyo.ac.jp/koudou/index.html
• [Remarks] 東京大学獣医動物行動学研究室
  • URL: http://www.vm.a.u-tokyo.ac.jp/koudou/index.html
• [Remarks]
  • URL: http://www.vm.a.u-tokyo.ac.jp/koudou/index.htm
• [Patent(Industrial Property Rights)] 臭気性組成物 (2014)
  • Inventor(s): 森裕司、武内ゆかり、清川泰志、稲垣秀晃、渡邉秀典
  • Industrial Property Rights Holder: 森裕司、武内ゆかり、清川泰志、稲垣秀晃、渡邉秀典
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-10-14