Functional analysis of ABA receptor-signaling complex

• Project/Area Number: 23688044
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: NISHIMURA Noriyuki 独立行政法人農業生物資源研究所, 放射線育種場, 研究員 (70405041)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2011: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
• Keywords: アブシジン酸 / シグナル伝達 / タンパク質脱リン酸化酵素 / 環境応答 / プロテオーム / 植物 / 分子間相互作用 / 植物ホルモン

Research Abstract

We pursued to identify the ABA receptors PYR/PYL/RCAR interacting proteins, post-translational modification sites in PYR/PYL/RCAR and natural ligands bind to PYR/PYL/RCAR. PYR/PYL/RCAR strongly interacts with cluster A PP2Cs and seems to be regulated by ubiqutination. The candidate natural ligands bind to PYR/PYL/RCAR has ABA like function but not ABA. The cluster A PP2Cs interact with different known ABA signaling components among them, suggested that plant conveniently selected the interactors to transduce ABA signaling.

Research Products

• [Journal Article] FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis (2014)
  • Author(s): Waadt R, Hitomi K, Nishimura N, Hitomi C, Adams S.R, Getzoff E.D, Schroeder J.I
  • Journal Title: eLife Volume: 3
  • DOI: 10.7554/elife.01739
  • Peer Reviewed / Open Access
• [Journal Article] Identification of cyclic GMP-activated nonselective Ca^2+-permeable cation channels and associated CNGC5 and CNGC6 genes in Arabidopsis guard cells (2013)
  • Author(s): Wang Y-F, Munemasa S, Nishimura N, Ren H-M, Robert N, Han M, Puznrjova I, Kollist H, Lee S, Mori I, Schroeder J.I
  • Journal Title: Plant Physiology Volume: 163(2) Issue: 2 Pages: 578-590
  • DOI: 10.1104/pp.113.225045
  • Peer Reviewed
• [Journal Article] Mutations in the SLAC1 anion channel slow stomatal opening and severely reduce K^+ uptake channel activity via enhanced cytosolic [Ca^2+] and increased Ca^<2+> sensitivity of K^+ uptake channels (2013)
  • Author(s): Laanemets K, Wang Y-F, Lindgren O, Wu J, Nishimura N, Lee S, Caddell D, Merilo E, Brosche M, Kilk K, Soomets U, Kangasjärvi J, Schroeder J.I, Kollist H
  • Journal Title: New Phytologist Volume: 197(1) Issue: 1 Pages: 88-98
  • DOI: 10.1111/nph.12008
  • Peer Reviewed
• [Journal Article] アブシジン酸受容とシグナル伝達機構の最前線 (2013)
  • Author(s): 佐藤浩二, 西村宜之
  • Journal Title: 植調 Volume: 47(2) Pages: 14-21
• [Journal Article] Isolation of Arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing. (2012)
  • Author(s): Murayama M, Hayashi S, Nishimura N, Ishide M, Kobayashi K, Yagi Y, Asami T, Nakamura T, Shinozaki K, Hirayama T
  • Journal Title: J. Exp. Bot. Volume: 63 Issue: 14 Pages: 5301-5310
  • DOI: 10.1093/jxb/ers188
  • Peer Reviewed
• [Journal Article] Chemical genetics reveals negative regulation of abscisic acid signaling by a plant immune response pathway (2011)
  • Author(s): Kim TH, Hauser F, Ha T, Xue S, Bohmer M, Nishimura N, Munemasa S, Hubbard K, Peine N, Lee BH, Lee S, Robert N, Parker JE, Schroeder JI
  • Journal Title: Current Biology Volume: 21 Issue: 11 Pages: 990-997
  • DOI: 10.1016/j.cub.2011.04.045
  • Peer Reviewed
• [Presentation] アブシジン酸シグナルで働くAHG1の相互作用因子の探索 (2013)
  • Author(s): 佐藤浩二, Moresco J, Tu P,林優紀,木下俊則, Schroeder J.I, Yates J.R,西村宜之
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-21
• [Presentation] アブシジン酸シグナルで働くAHG1の相互作用因子の探索 (2013)
  • Author(s): 佐藤浩二
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学 津島キャンパス
• [Presentation] アブシジン酸受容および情報伝達機構に関する研究 (2012)
  • Author(s): 西村宜之
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] アブシジン酸受容および情報伝達機構に関する研究 (2012)
  • Author(s): 西村宜之
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)(受賞講演)
  • Year and Date: 2012-03-17
• [Presentation] Proteomic analysis of ABA perception and early ABA signaling mechanisms (2011)
  • Author(s): Nishimura N, Hitomi K, Arvai A.S, Rambo R.P, Wang A, Lee S, Caddell D.F, Sarkeshik A, Nito K, Park S-Y, Hitomi C, Carvalho P.C, Chory J, Yates J.R,III, Cutler S.R, Getzoff E.D, Schroeder J.I
  • Organizer: 3rd International Symposium Frontiers in Agriculture Proteome Research:Contribution of proteomics technology in agricultural sciences
  • Place of Presentation: つくば
  • Year and Date: 2011-11-08
• [Presentation] Proteomic analysis of ABA perception and early ABA signaling mechanisms (2011)
  • Author(s): Nishimura N, Hitomi K, Arvai AS, Rambo RP, Wang A, Lee S, Caddell DF, Sarkeshik A, Nito K, Park SY, Hitomi C, Carvalho PC, Chory J, Yates, JR, Cutler SR, Getzoff ED, Schroeder JI
  • Organizer: 3nd International Symposium on Frontiers in Agriculture Proteome Research
  • Place of Presentation: つくば国際会議場(茨城)(招待講演)
  • Year and Date: 2011-11-08
• [Presentation] アブシジン酸シグナリングネットワークを介した植物のストレス耐性機構 (2011)
  • Author(s): 西村宜之
  • Organizer: 藪田セミナー「化学物質による植物のストレス耐性の制御
  • Place of Presentation: 東京
  • Year and Date: 2011-07-14
• [Presentation] アブシジン酸シグナリングネットワークを介した植物のストレス耐性機構 (2011)
  • Author(s): 西村宜之
  • Organizer: 薮田セミナー「化学物質による植物のストレス耐性の制御」
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2011-07-14
• [Presentation] アブシジン酸受容体PYR/PYL/RCARファミリーを介したアブシジン酸情報伝達機構 (2011)
  • Author(s): 西村宜之
  • Organizer: 第4回農芸化学の未来開拓セミナー
  • Place of Presentation: 岡山
  • Year and Date: 2011-05-20
• [Presentation] アブシジン酸受容体PYR/PYL/RCARファミリーを介したアブシジン酸情報伝達機構 (2011)
  • Author(s): 西村宜之
  • Organizer: 第4回農芸化学の未来開拓セミナー
  • Place of Presentation: 岡山大学(岡山)(招待講演)
  • Year and Date: 2011-05-20
• [Book] アブシジン酸受容とシグナル伝達機構の最前線植調47(2): (2013)
  • Author(s): 佐藤浩二,西村宜之
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/org/GR/IRB/
• [Remarks] 2012年度日本植物生理学会奨励賞受賞