Investigation on the molecular basis of neuropeptide receptors for the regulation of reproductive systems

• Project/Area Number: 23689007
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Drug development chemistry
• Research Institution: Kyoto University
• Principal Investigator: OISHI Shinya 京都大学, 薬学研究科(研究院), 講師 (80381739)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 性ホルモン / キスペプチン / GPR54 / ニューロキニン / 受容体 / プローブ

Outline of Final Research Achievements

Novel selective ligands and specific molecular probes for kisspeptin receptor (GPR54) and neurokinin receptor were developed. Fluorescent probes and photoaffinity probes for kisspeptin receptor were designed and synthesized. These probes were applicable to the detection of GPR54 in confocal microscopy analysis, flow cytometry and photolabeling experiments. Additionally, novel NK3 receptor agonists with resistance against peptidase-mediated degradation were developed via the structure-activity relationship studies using a number of peptidomimetics.

Research Products

• [Journal Article] Structure-activity relationship study on senktide for development of novel potent neurokinin-3 receptor selective agonists. (2015)
  • Author(s): Misu R, Yamamoto K, Yamada A, Noguchi T, Ohno H, Yamamura T, Okamura H, Matsuda F, Ohkura S, Oishi S, Fujii N.
  • Journal Title: MedChemCommun Volume: 6 Issue: 3 Pages: 469-476
  • DOI: 10.1039/c4md00514g
  • Peer Reviewed / Open Access
• [Journal Article] Development of a novel neurokinin-3 receptor selective agonist with highly biological stability (2015)
  • Author(s): Misu R, Oishi S, Yamada A, Yamamoto K, Noguchi T, Ohno H, Yamamura T, Okamura H, Matsuda F, Ohkura S, Fujii N.
  • Journal Title: Peptide Science Volume: 2014 Pages: 23-24
  • Peer Reviewed
• [Journal Article] Optimization of neurokinin-3 receptor (NK3R)-selective agonists (2015)
  • Author(s): Yamamoto K, Oishi S, Misu R, Noguchi T, Ohno H, Yamamura T, Okamura H, Fujii N.
  • Journal Title: Peptide Science Volume: 2014 Pages: 241-242
  • Peer Reviewed
• [Journal Article] Design and synthesis of fluorescent probes for GPR54. (2014)
  • Author(s): Kaneda M, Misu R, Ohno H, Hirasawa A, Ieda N, Uenoyama Y, Tsukamura H, Maeda K, Oishi S, Fujii N.
  • Journal Title: Bioorg. Med. Chem. Volume: 22 Issue: 13 Pages: 3325-3330
  • DOI: 10.1016/j.bmc.2014.04.052
  • Peer Reviewed
• [Journal Article] Development of novel neurokinin 3 receptor (NK3R) selective agonists with resistance to proteolytic degradation. (2014)
  • Author(s): Misu R, Oishi S, Yamada A, Yamamura T, Matsuda F, Yamamoto K, Noguchi T, Ohno H, Okamura H, Ohkura S, Fujii N.
  • Journal Title: J. Med. Chem. Volume: 57 Issue: 20 Pages: 8646-8651
  • DOI: 10.1021/jm500771w
  • Peer Reviewed
• [Journal Article] A radiogallium-DOTA-based bivalent peptidic ligand targeting a chemokine receptor, CXCR4, for tumor imaging (2014)
  • Author(s): Sano K, Masuda R, Hisada H, Oishi S, Shimokawa K, Ono M, Fujii N, Saji H, Mukai T.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 24 Issue: 5 Pages: 1386-1388
  • DOI: 10.1016/j.bmcl.2014.01.031
  • Peer Reviewed
• [Journal Article] An optimized method of G-protein coupled receptor homology modeling: its application to the discovery of novel CXCR7 ligands (2013)
  • Author(s): Yoshikawa Y, Oishi S, Kubo T, Tanahara N, Fujii N, Furuya T.
  • Journal Title: J. Med. Chem. Volume: 56 Issue: 11 Pages: 4236-4251
  • DOI: 10.1021/jm400307y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Chronic Peripheral Administration of Kappa-Opioid Receptor Antagonist Advances Puberty Onset Associated with Acceleration of Pulsatile Luteinizing Hormone Secretion in Female Rats (2013)
  • Author(s): 中原辰夫
  • Journal Title: Journal of Reproduction and Development Volume: 59 Issue: 5 Pages: 479-484
  • DOI: 10.1262/jrd.2013-046
  • NAID: 130003361050
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Effects of Full-Length Kisspeptin Administration on Follicular Development in Japanese Black Beef Cows (2013)
  • Author(s): Naniwa, Y., Nakatsukasa, K., Setsuda, S., Oishi, S., Fujii, N., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K. -I. and Ohkura, S.
  • Journal Title: Journal of Reproduction and Development Volume: 59 Issue: 6 Pages: 588-594
  • DOI: 10.1262/jrd.2013-064
  • NAID: 130003381852
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Structure-activity relationship study of tachykinin peptides for the development of novel neurokinin-3 receptor selective agonists. (2013)
  • Author(s): Misu R, Noguchi T, Ohno H, Oishi S, Fujii N.
  • Journal Title: Bioorg. Med. Chem. Volume: 21 Issue: 8 Pages: 2413-2417
  • DOI: 10.1016/j.bmc.2013.01.036
  • Peer Reviewed
• [Journal Article] Characterization of the receptor binding residues of kisspeptins by positional scanning using peptide photoaffinity probes (2013)
  • Author(s): Misu R, Oishi S, Setsuda S, Noguchi T, Kaneda M, Ohno H, Evans B, Navenot JM, Peiper SC, Fujii N.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 23 Issue: 9 Pages: 2628-2631
  • DOI: 10.1016/j.bmcl.2013.02.098
  • Peer Reviewed
• [Journal Article] Paradoxical downregulation of CXC chemokine receptor 4 induced by polyphemusin II-derived antagonists. (2012)
  • Author(s): Masuda R, Oishi S, Tanahara N, Ohno H, Hirasawa A, Tsujimoto G, Yano Y, Matsuzaki K, Navenot J, Peiper S, Fujii N.
  • Journal Title: Bioconjug. Chem. Volume: 23 Issue: 6 Pages: 1259-1265
  • DOI: 10.1021/bc300084h
  • Peer Reviewed
• [Journal Article] CXCR4 stimulates macropinocytosis: implications for cellular uptake of arginine-rich cell-penetrating peptides and HIV (2012)
  • Author(s): Gen Tanaka, Ikuhiko Nakase, et al.
  • Journal Title: Chemistry & Biology Volume: 19 Issue: 11 Pages: 1437-1446
  • DOI: 10.1016/j.chembiol.2012.09.011
  • Peer Reviewed
• [Journal Article] The metastasis suppressor KISS1 lacks antimetastatic activity in the C8161.9 xenograft model of melanoma (2012)
  • Author(s): Navenot JM, Evans B, Oishi S, Setsuda S, Fujii N, Peiper SC.
  • Journal Title: Melanoma Res. Volume: 22 Issue: 2 Pages: 140-150
  • DOI: 10.1097/cmr.0b013e328350fa07
  • Peer Reviewed
• [Journal Article] Development and application of fluorescent SDF-1 derivatives (2012)
  • Author(s): Masuda R, Oishi S, Tanahara N, Ohno H, Hirasawa A, Tsujimoto G, Kodama E, Matsuoka M, Fujii N.
  • Journal Title: Future Med. Chem. Volume: 4 Issue: 7 Pages: 837-844
  • DOI: 10.4155/fmc.12.31
  • NAID: 120005285488
  • Peer Reviewed
• [Journal Article] Structure-Activity Relationship Study of a CXC Chemokine Receptor Type 4 Antagonist, FC131, Using a Series of Alkene Dipeptide Isosteres (2012)
  • Author(s): Kobayashi K., et al
  • Journal Title: Journal of Medicinal Chemistry Volume: (in press) Issue: 6 Pages: 2746-2757
  • DOI: 10.1021/jm2016914
  • Peer Reviewed
• [Journal Article] Kisspeptin neurons mediate reflex ovulation in the musk shrew (Suncus murinus). (2011)
  • Author(s): Inoue, N. Sasagawa, K. Ikai, K. Sasaki, Y. Tomikawa, J. Oishi, S. Fujii, N. Uenoyama, Y. Ohmori, Y. Yamamoto, N. Hondo, E. Maeda, K. Tsukamura, H.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 108 Issue: 42 Pages: 17527-17532
  • DOI: 10.1073/pnas.1113035108
  • Peer Reviewed
• [Journal Article] Concise site-specific synthesis of DTPA-peptide conjugates: application to imaging probes for the chemokine receptor CXCR4 (2011)
  • Author(s): Masuda R, Oishi S, Ohno H, Kimura H, Saji H, Fujii N.
  • Journal Title: Bioorg. Med. Chem. Volume: 19 Issue: 10 Pages: 3216-3220
  • DOI: 10.1016/j.bmc.2011.03.059
  • NAID: 120004770226
  • Peer Reviewed
• [Presentation] 新規ニューロキニン-3受容体アゴニストの創製と繁殖中枢制御への応用 (2015)
  • Author(s): 山本昂輝、大石真也、三須良介、野口太朗、大野浩章、山村崇、岡村裕昭、藤井信孝
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 兵庫医療大学（兵庫県神戸市）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Optimization of neurokinin-3 receptor (NK3R)-selective agonists (2014)
  • Author(s): Koki Yamamoto, Shinya Oishi, Ryosuke Misu, Taro Noguchi, Hiroaki Ohno, Takashi Yamamura, Hiroaki Okamura, Nobutaka Fujii
  • Organizer: 第51回ペプチド討論会
  • Place of Presentation: 徳島大学（徳島県徳島市）
  • Year and Date: 2014-10-22 – 2014-10-24
• [Presentation] Development of novel neurokinin-3 receptor selective agonists with resistance against proteolytic degradation (2014)
  • Author(s): Ryosuke Misu, Shinya Oishi, Ai Yamada, Koki Yamamoto, Taro Noguchi, Hiroaki Ohno, Takashi Yamamura, Hiroaki Okamura, Fuko Matsuda, Satoshi Ohkura, Nobutaka Fujii
  • Organizer: 第51回ペプチド討論会
  • Place of Presentation: 徳島大学（徳島県徳島市）
  • Year and Date: 2014-10-22 – 2014-10-24
• [Presentation] 新規ニューロキニン-3受容体アゴニストの創製研究 (2014)
  • Author(s): 山本昂輝、大石真也、三須良介、野口太朗、大野浩章、山村崇、岡村裕昭、藤井信孝
  • Organizer: 創薬懇話会2014 in 岐阜
  • Place of Presentation: ぎふ長良川温泉 ホテルパーク（岐阜県岐阜市）
  • Year and Date: 2014-07-10 – 2014-07-11
• [Presentation] 新規ニューロキニン-3受容体アゴニストの創製研究 (2014)
  • Author(s): 山本昂輝、大石真也、三須良介、野口太朗、大野浩章、山村崇、岡村裕昭、藤井信孝
  • Organizer: 日本ケミカルバイオロジー学会 第9回年会
  • Place of Presentation: 大阪大学（大阪府豊中市）
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] ペプチダーゼ耐性を有する新規NK3受容体アゴニストの創製研究 (2014)
  • Author(s): 三須良介、大石真也、山田愛、山本昂輝、野口太朗、大野浩章、山村崇、岡村裕昭、松田二子、大蔵聡、藤井信孝
  • Organizer: 日本ケミカルバイオロジー学会 第9回年会
  • Place of Presentation: 大阪大学（大阪府豊中市）
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] ニューロキニン-3受容体アゴニストの構造活性相関研究 (2014)
  • Author(s): 山本昂輝、三須良介、野口太朗、大野浩章、大石真也、山村崇、岡村裕昭、藤井信孝
  • Organizer: 第11回GPCR研究会
  • Place of Presentation: 日本科学未来館（東京都江東区）
  • Year and Date: 2014-05-09 – 2014-05-10
• [Presentation] NK3受容体アゴニストの生体内安定性評価と新規誘導体の創製 (2014)
  • Author(s): 三須良介、大石真也、山田愛、山本昂輝、野口太朗、大野浩章、山村崇、岡村裕昭、松田二子、大蔵聡、藤井信孝
  • Organizer: 第11回GPCR研究会
  • Place of Presentation: 日本科学未来館（東京都江東区）
  • Year and Date: 2014-05-09 – 2014-05-10
• [Presentation] Structure-activity relationship study of tachykinin peptides for develpment of novel neurokinin-3 receptor agonists (2012)
  • Author(s): Misu R, Oishi S, Noguchi T, Yamada A, Ohno H, Yamamura T, Okamura H, Matsuda F, Ohkura S, Fujii N.
  • Organizer: 2nd World Conference on Kisspeptin Signaling in the Brain
  • Place of Presentation: 東京大学（東京都）
• [Presentation] Structure-activity relationship study of neurokinin-3 receptor agonists (2012)
  • Author(s): Noguchi T, Oishi S, Misu M, Ohno H, Yamamura T, Okamura H, Fujii N.
  • Organizer: 第49回ペプチド討論会
  • Place of Presentation: 鹿児島県民交流センター（鹿児島県）
• [Presentation] アミジン型ペプチド結合等価体の合成と応用 (2011)
  • Author(s): 井ノ口恵利子、山田 愛、大石真也、久保達彦、大野浩章、保住健太郎、野水基義、志村和也、松岡雅雄、藤井信孝
  • Organizer: 第6回日本ケミカルバイオロジー学会年会
  • Place of Presentation: 東京工業大学大岡山キャンパス（東京都）
• [Presentation] GPR54リガンドによるNPFF受容体の活性化 (2011)
  • Author(s): 三須良介、大石真也、富田健嗣、説田章平、増田亮、大野浩章、難波陽介、家田菜穂子、井上直子、大蔵聡、上野山賀久、束村博子、前多敬一郎、平澤明、辻本豪三、藤井信孝
  • Organizer: 第6回日本ケミカルバイオロジー学会年会
  • Place of Presentation: 東京工業大学大岡山キャンパス（東京都）
• [Presentation] Synthesis and biological evaluation of novel CXCR4 antagonists containing amidine-type peptide bond isosteres (2011)
  • Author(s): Inokuchi E, Oishi S, Kubo T, Ohno H, Shimura K, Matsuoka M, Fujii N.
  • Organizer: 22nd American Peptide Symposium
  • Place of Presentation: San Diego（United States of America）
• [Presentation] Synthesis and application of fluorescent SDF-1 derivatives (2011)
  • Author(s): Masuda R, Oishi S, Tanahara N, Ohno H, Hirasawa A, Tsujimoto G, Yano Y, Matsuzaki K, Fujii N.
  • Organizer: 22nd American Peptide Symposium
  • Place of Presentation: San Diego（United States of America）
• [Presentation] Structure-activity relationship study on a CXCR4 antagonist FC131 using alkene-type dipeptide isosteres (2011)
  • Author(s): Kobayashi K, Oishi S, Hayashi R, Tomita K, Kubo T, Tanahara N, Ohno H, Naito N, Matsuoka M, Fujii N.
  • Organizer: 22nd American Peptide Symposium
  • Place of Presentation: San Diego（United States of America）
• [Presentation] Internalization of CXC chemokine receptor 4 (CXCR4) induced by polyphemusin II-derived antagonist peptides (2011)
  • Author(s): Masuda R, Oishi S, Tanahara N, Ohno H, Hirasawa A, Tsujimoto G, Yano Y, Matsuzaki K, Nakase I, Futaki S, Fujii N.
  • Organizer: 第48回ペプチド討論会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
• [Presentation] Development and application of fluorescent probes for monitoring CXCR4 receptor internalization (2011)
  • Author(s): Oishi S, Masuda R, Tanahara N, Ohno H, Yano Y, Matsuzaki K, Hirasawa H, Tsujimoto G, Fujii N.
  • Organizer: Nature Chemical Biology Symposium 2011: Cancer Chemical Biology
  • Place of Presentation: Boston（United States of America）
• [Presentation] アルケン型及びアミジン型ジペプチドイソスターの合成とCXCR4拮抗剤の構造活性相関研究への応用 (2011)
  • Author(s): 小林数也、大石真也、井ノ口恵利子、林亮子、富田健嗣、久保達彦、棚原憲子、大野浩章、藤井信孝、吉川寧、古谷利夫、志村和也、松岡雅雄
  • Organizer: 第37回 反応と合成の進歩シンポジウム
  • Place of Presentation: あわぎんホール（徳島県）
• [Presentation] Potent CXCR4 antagonists containing amidine-type peptide bond isosteres (2011)
  • Author(s): Kubo T, Inokuchi E, Oishi S, Ohno H, Shimura K, Matsuoka M, Fujii N.
  • Organizer: AIMECS11
  • Place of Presentation: 京王プラザホテル（東京都）
• [Presentation] 光アフィニティープローブを利用したキスペプチン類の受容体結合残基の同定
  • Author(s): 三須良介、大石真也、説田章平、野口太朗、金田雅仁、大野浩章、藤井信孝
  • Organizer: 第8回ケミカルバイオロジー学会年会
  • Place of Presentation: 東京医科歯科大学（東京都文京区）
• [Presentation] Structure-activity Relationship Study of Tachykinin Peptides for Development of Novel Neurokinin-3 Receptor Selective Agonists
  • Author(s): Ryosuke Misu, Shinya Oishi, Taro Noguchi, Ai Yamada, Hiroaki Ohno, Fuko Matsuda, Satoshi Ohkura, Nobutaka Fujii
  • Organizer: 23rd American Peptide Symposium
  • Place of Presentation: ヒルトンワイコロアホテル（米国、コナ市）
• [Presentation] Development of Photoaffinity Probes for Characterization of the Receptor Binding Residues in Kisspeptins
  • Author(s): Shinya Oishi, Shohei Setsuda, Ryosuke Misua Taro Noguchi, Masato Kaneda, Hiroaki Ohno, Barry Evans, Jean-Marc Navenot, Stephen C. Peiper, Nobutaka Fujii
  • Organizer: 23rd American Peptide Symposium
  • Place of Presentation: ヒルトンワイコロアホテル（米国、コナ市）
• [Presentation] ウイルス変異に基づく抗HIV活性ペプチドのデザイン
  • Author(s): 大石真也
  • Organizer: 生命分子機能研究会2013学術集会
  • Place of Presentation: 長浜バイオ大学（滋賀県長浜市）
  • Invited
• [Presentation] Synthesis and functional characterization of fluorescent probes for GPR54
  • Author(s): Masato Kaneda, Ryosuke Misu, Shinya Oishi, Hiroaki Ohno, and Nobutaka Fujii
  • Organizer: 2nd Annual Conference ICBS2013
  • Place of Presentation: 京都大学芝蘭会館（京都府京都市）
• [Presentation] Characterization of the Receptor Binding Residues of Kisspeptins by Using Photoaffinity Probes
  • Author(s): Ryosuke Misu, Shinya Oishi, Shohei Setsuda, Taro Noguchi, Masato Kaneda, Hiroaki Ohno, Barry Evans, Jean-Marc Navenot, Stephen C. Peiper, Nobutaka Fujii
  • Organizer: 2nd Annual Conference ICBS2013
  • Place of Presentation: 京都大学芝蘭会館（京都府京都市）
• [Presentation] 生体機能の解明に向けたものづくり：生物活性環状ペプチドの設計と応用
  • Author(s): 大石真也
  • Organizer: 第3回化学フェスタ
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Invited
• [Presentation] Design and synthesis of fluorescent probes for GPR54
  • Author(s): Masato Kaneda, Ryosuke Misu, Shinya Oishi, Hiroaki Ohno, and Nobutaka Fujii
  • Organizer: 4th Asia-Pacific International Peptide Symposium
  • Place of Presentation: ホテル阪急エキスポパーク（大阪府吹田市）
• [Presentation] Evaluation of Biological Stability of Neurokinin-3 Receptor Agonists for the Development of Novel Biostable Analogs
  • Author(s): Ryosuke Misu, Shinya Oishi, Taro Noguchi, Ai Yamada, Hiroaki Ohno, Fuko Matsuda, Satoshi Ohkura, Nobutaka Fujii
  • Organizer: 4th Asia-Pacific International Peptide Symposium
  • Place of Presentation: ホテル阪急エキスポパーク（大阪府吹田市）
• [Presentation] キスペプチン受容体に対する蛍光プローブの創製研究
  • Author(s): 金田雅仁、大石真也、三須良介、大野浩章、藤井信孝
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学黒髪キャンパス（熊本県熊本市）
• [Presentation] NK3受容体リガンドの安定性評価と新規選択的アゴニストの創製
  • Author(s): 山田愛、三須良介、大石真也、大野浩章、松田二子、大蔵聡、藤井信孝
  • Organizer: 第62回日本薬学会近畿支部大会
  • Place of Presentation: 武庫川女子大学（兵庫県）
• [Presentation] 縮環インドール類の新しい生物活性の探索：KSP阻害剤の創製
  • Author(s): 大石真也
  • Organizer: 有機合成化学を起点とするものづくり戦略ミニシンポジウム
  • Place of Presentation: 東京大学（東京都）
  • Invited
• [Remarks] 所属研究室ホームページ
  • URL: http://www.pharm.kyoto-u.ac.jp//seizo/
• [Remarks] 所属研究室ホームページ
  • URL: http://www.pharm.kyoto-u.ac.jp//seizo/
• [Remarks] 所属研究室ホームページ
  • URL: http://www.pharm.kyoto-u.ac.jp/seizo/
• [Remarks] 所属研究室ホームページ
  • URL: http://www.pharm.kyoto-u.ac.jp/seizo/
• [Patent(Industrial Property Rights)] 新規NK3受容体アゴニスト (2014)
  • Inventor(s): 藤井信孝、大野浩章、大石真也、三須良介、山田愛、山本昂輝、他
  • Industrial Property Rights Holder: 国立大学法人京都大学、独立行政法人農業生物資源研究所
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-12-05
  • Overseas
• [Patent(Industrial Property Rights)] 新規NK3受容体アゴニスト (2013)
  • Inventor(s): 藤井信孝、大石真也、他
  • Industrial Property Rights Holder: 国立大学法人京都大学、独立行政法人農業生物資源研究所
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-10-18
  • Overseas
• [Patent(Industrial Property Rights)] 新規NK3受容体アゴニスト (2013)
  • Inventor(s): 藤井信孝、大石真也、他
  • Industrial Property Rights Holder: 国立大学法人京都大学、独立行政法人農業生物資源研究所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-253534
  • Filing Date: 2013-12-06
• [Patent(Industrial Property Rights)] 新規NK3受容体アゴニスト (2012)
  • Inventor(s): 藤井信孝、大野浩章、大石真也、野口太朗、三須良介
  • Industrial Property Rights Holder: 藤井信孝、大野浩章、大石真也、野口太朗、三須良介
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-231902
  • Filing Date: 2012-10-19