| Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
|---|
| Outline of Final Research Achievements |
Selective protein degradation is crucial in regulation of various cellular functions. The degradation machinery is supposed to play important roles in intracellular transport. To address this issue, we studied the regulation of the dynamics of motor protein by the ubiquitin ligase, especially focused on SCRAPPER, which we previously identified (Yao et al., Cell 2007)
We revealed reciprocal interactions between SCRAPPER and the motor protein and found that the amount of the motor protein was regulated by SCRAPPER dependent ubiquitination and the proteasomal degradation. With mass spectrometry imaging technique, we also identified some candidates of molecules that showed significant modulation in the brain sections of Scrapper gene knockout mice compared with those of wild type mice.
|
