Study on mechanisms for formation of genome replication vesicles produced by plus-strand RNA viruses

• Project/Area Number: 23689028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MORI Yoshio 国立感染症研究所, その他部局等, 室長 (40379095)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: ウイルス / 細胞 / プラス鎖RNAウイルス / 風疹ウイルス / ゲノム複製機構 / アクチン / 細胞骨格 / ゲノム複製 / 形質膜

Research Abstract

The common hallmark of plus-strand RNA viruses is to synthesize their genomes at various organelle membranes. In this study, we attempted to isolate viral and host factors for genome synthesis of plus-strand RNA viruses and common mechanisms underlying genome replication. In order to quantify, the subgenomic replicon of rubella virus, a member of plus-strand RNA viruses, was established in this study, and using this system viral and host factors for promoting viral propagation were identified. Furthermore, this is the first study to indicate that rubella virus undergoes the genome synthesis at the plasma membrane. The results in this study provide the information for understanding of mechanisms underlying genome replication of plus-strand RNA viruses.

Research Products

• [Journal Article] Immuno-Electron Microscopy of Primary Cell Cultures from GeneticallyModified Animals in Liquid by Atmospheric Scanning Electron Microscopy (ASEM) (2014)
  • Author(s): T.Kinoshita, Y.Mori, K.Hirano, S.Sugimoto, K.Okuda, S.Matsumoto, T.Namiki, T.Ebihara, M.Kawata, H.Nishiyama, M.Sato, M.Suga, K.Higashiyama, K.Sonomoto, Y.Mizunoe, S.Nishihara, C.Sato
  • Journal Title: Microscopy and Microanalysis Volume: 20 Issue: 2 Pages: 469-483
  • DOI: 10.1017/s1431927614000178
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation (2013)
  • Author(s): Katoh H, Okamoto T, Fukuhara T, Kambara H, Morita E, Mori Y, Kamitani W, Matsuura Y
  • Journal Title: J.Virol Volume: 87 Pages: 489-502
• [Journal Article] Immuno-electron microscopy of primary cell cultures from genetically modified animals in liquid by atmospheric scanning electron microscopy (2013)
  • Author(s): Kinoshita T, Mori Y, Hirano K, Sugimoto S, Okuda K, Matsumoto S, Namiki, Ebihara R, Kawata M, Nishiyama H, Sato M, Suga M, Higashiyama K, Sonomoto S, Mizunoe Y, Nishihara S, Sato C
  • Journal Title: Microscopy and microanalysis Volume: 20 Pages: 469-483
• [Journal Article] Nationwide Rubella (2013)
  • Author(s): Tanaka-Taya K, Sato H, Arai S, Yamagishi T Yahata Y, Nakayama K, Sugawara T Ohkusa Y, Matsui T, Saito T, Kanou K, Shimada T, Kinoshita H, Yamashita K, Yasui Y, Tada Y, Mori Y, Takeda M, Sunagawa T, Oishi K
  • Journal Title: Epidemic-Japan Volume: 62(27) Pages: 457-462
• [Journal Article] Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation. (2013)
  • Author(s): Hiroshi Katoh
  • Journal Title: Journal of Virology Volume: 87(1) Issue: 1 Pages: 489-502
  • DOI: 10.1128/jvi.02186-12
  • Peer Reviewed
• [Journal Article] Nationwide Rubella Epidemic – Japan, 2013 (2013)
  • Author(s): Tanaka-Taya K, Satoh H, Arai S, Yamagishi T, Yahata Y, Nakashima K, Sugawara T, Ohkusa Y, Matsui T, Saito T, Kanou K, Shimada T, Kinoshita H, Yamashita K, Yasui Y, Tada Y, Mori Y, Takeda M, Sunagawa T, Oishi K.
  • Journal Title: Moorb. Mortal. Wkly. Rep Volume: 62(27) Pages: 457-462
  • Peer Reviewed
• [Journal Article] Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA (2011)
  • Author(s): Katoh H, Mori Y, Kambara H, Abe T, Fukuhara T, Morita E, Moriishi K, Kamitani W, Matsuura Y
  • Journal Title: J.Virol Volume: 85(21) Pages: 10976-10988
• [Journal Article] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H, Tani H, Mori Y, Abe T, Katoh H, Fukuhara T, Taguwa S, Moriishi K, Matsuura Y
  • Journal Title: Virology Volume: 412(1)
• [Journal Article] Elucidation of the Full Genetic Information of Japanese Rubella Vaccines and the Genetic Changes Associated with In Vitro and In Vivo Vaccine Virus Phenotypes (2011)
  • Author(s): Otsuki N, Abo H, Kubota T, Mori Y, Umino Y, Okamoto K, Takeda M, Komase K
  • Journal Title: Vaccine Volume: 29(10) Pages: 1863-1873
• [Journal Article] Heterogeneous Nuclear Ribonucleoprotein A2 Participates in the Replication of Japanese Encephalitis Virus through an Interaction with Viral Proteins and RNA (2011)
  • Author(s): Hiroshi Katoh
  • Journal Title: Journal of Virology Volume: 85(21) Issue: 21 Pages: 10976-10988
  • DOI: 10.1128/jvi.00846-11
  • Peer Reviewed
• [Journal Article] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H., Tani H., Mori Y., Abe T., Katoh H., Fukuhara T., Taguwa S., Moriishi K, and Matsuura Y.
  • Journal Title: Virology Volume: 412 Issue: 1 Pages: 211-219
  • DOI: 10.1016/j.virol.2011.01.011
  • Peer Reviewed
• [Presentation] Molecular epidemiology and laboratory diagnosis of rubella virus in Japan (2013)
  • Author(s): Mori Y
  • Organizer: The 10^<th> Japan-Taiwan symposium on vaccine preventable diseases and vector-borne diseases
• [Presentation] (2013)
  • Author(s): Mori Y
  • Organizer: The 7^<th> China-Korea-Japan forum on communicable disease control and prevention
• [Presentation] Molecular epidemiology and laboratory diagnosis of rubella in Japan. (2013)
  • Author(s): Yoshio Mori
  • Organizer: The 10th Japan-Taiwan symposium on vaccine preventable diseases and vector-borne diseases
  • Place of Presentation: 東京 国立感染症研究所
• [Presentation] Epidemiological Analysis on rubella epidemic in Japan. (2013)
  • Author(s): Yoshio Mori
  • Organizer: The 7th China-Korea-Japan forum on communicable disease control and prevention. Beijing China
  • Place of Presentation: 中国 北京
• [Presentation] 風疹ウイルスCタンパク質とp150タンパク質の共局在がウイルス産生に与える影響 (2012)
  • Author(s): 坂田真史、岡本貴世子、大槻紀之、安楽正輝、竹田誠、森嘉生
  • Organizer: 第60回日本ウイルス学会学術集会
• [Presentation] N-terminal hydrophobic amino acid residues of the capsid protein are critical for co-localization with the p150 protein and production of rubella virus (2012)
  • Author(s): Sakata M, Okamoto K, Otsuki N, Anraku M, Takeda M, Mori Y
  • Organizer: 11^<th> Awaji International forum on infection and immunity
• [Presentation] Rubella virus undergoes genome synthesis at the plasma membrane with induction of filopodia (2012)
  • Author(s): Mori Y, Okamoto K, Sakata M, Otsuki N, Takeda M
  • Organizer: 34^<th> Naito conference
• [Presentation] 風疹ウイルスCタンパク質とp150タンパク質の共局在がウイルス産生に与える影響 (2012)
  • Author(s): 坂田真史、岡本貴世子、大槻紀之、安楽正輝、竹田誠、（森嘉生）
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪市
• [Presentation] N-terminal hydrophobic amino acid residues of the capsid protein are critical for co-localization with the p150 protein and production of rubella virus (2012)
  • Author(s): Masafumi Sakata, Kiyoko Okamoto, Noriyuki Otsuki, Masaki Anraku, Makoto Takeda, and (Yoshio Mori)
  • Organizer: 11th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路市
• [Presentation] Rubella virus undergoes genome synthesis at the plasma membrane with induction of filopodia (2012)
  • Author(s): (Yoshio Mori), Masafumi Sakata, Kiyoko Okamoto, Noriyuki Otsuki, Makoto Takeda
  • Organizer: 34th Naito conference
  • Place of Presentation: 札幌市
• [Presentation] Heterogeneous Nuclear Ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through the interation with viral proteins and RNA (2011)
  • Author(s): Katoh H., et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-15
• [Presentation] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H., et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-15
• [Presentation] The short N-terminal region of the rubella virus capsid protein is critical to co-localize with the nonstructural p150 protein (2011)
  • Author(s): Sakata M., et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-13
• [Presentation] TMolecular Mechanisms of the temperature-sensitive phenotype of live attenuated Japanese rubella vaccines (2011)
  • Author(s): Otsuki N., et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-13
• [Presentation] The plasma membrane is the genome replication site for rubella virus (2011)
  • Author(s): Mori Y., et al
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-12
• [Presentation] The plasma membrane is the genome replication site for rubella virus (2011)
  • Author(s): Mori Y, Okamoto K, Sakata M, Otsuki N, Abo H, Takeda M
  • Organizer: XV International Congress of Virology
• [Presentation] The short N-terminal region of the rubella virus capsid protein is critical to co-localize with the nonstructural p150 protein (2011)
  • Author(s): Sakata M, Okamoto K, Otsuki N, Abo H, Takeda M, Mori Y
  • Organizer: XV International Congress of Virology
• [Presentation] Molecular mechanisms of the temperature-sensitive phenotype of live attenuated Japanese rubella vaccines (2011)
  • Author(s): Otsuki N, Sakata M, Okamoto K, Abo H, Kano K, Komase K, Takeda M, Mori Y
  • Organizer: XV International Congress of Virology
• [Presentation] Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA (2011)
  • Author(s): Katoh H, Mori Y, Kambara H, Kamitani W, Matsuura Y
  • Organizer: XV International Congress of Virology
• [Presentation] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H, Tani H, Mori Y, Abe T, Katoh H, Fukuhara T, Taguwa S, Moriishi K, Matsuura Y
  • Organizer: XV International Congress of Virology