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Recognition mechanism of DNA by innate immunity

Research Project

Project/Area Number 23689030
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionNara Institute of Science and Technology (2013)
Osaka University (2011-2012)

Principal Investigator

KAWAI Taro  奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (50456935)

Research Collaborator JIAN Zou  大阪大学, 大学院医学系研究科, 博士課程
SURYA Pandey  大阪大学, 大学院医学系研究科, 博士課程
TAKESHI Kondo  大阪大学, 大学院医学系研究科, 博士課程
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords自然免疫 / DNAセンサー / シグナル伝達 / インターフェロン / ウイルス / 自己免疫疾患 / ウイルス感染
Research Abstract

Upon recognition of double-stranded DNA derived from virus or bacteria by innate immunity, host cells produce type I interferon or other inflammatory mediators essential for host defense. However, mechanisms involved in DNA recognition are unclear. In addition, it is suggested that self-DNA derived from damaged cells are recognized by innate immunity and this is considered to be responsible for development of inflammatory and autoimmune diseases. In this research, we identified MRE11, a protein involved in repair responses, as a candidate protein involved in self-DNA recognition and induction of type I interferon and inflammatory cytokines. MRE11 binds to and colocalizes with immunostimulatory DNA, and cells that do not express MRE11 have defects in induction of these cytokines by immunostimulatory DNA. These findings may shed light on our understanding of pathogenesis of autoimmunity in which aberrant DNA recognition is involved.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (27 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (6 results) (of which Invited: 4 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome.2014

    • Author(s)
      Takemura, N., et al.
    • Journal Title

      Nature Commun.

      Volume: 5 Issue: 1 Pages: 3492-3492

    • DOI

      10.1038/ncomms4492

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The second messenger phosphatidylinositol-5-phosphate facilitates antiviral innate immune signaling2013

    • Author(s)
      Kawasaki T, Takemura N, Standley DM, Akira S, Kawai T
    • Journal Title

      Cell Host Microbe

      Volume: 14;14(2) Issue: 2 Pages: 148-58

    • DOI

      10.1016/j.chom.2013.07.011

    • NAID

      120005716837

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Completion of the entire hepatitis C virus life cycle in genetically humanized mice2013

    • Author(s)
      Dorner M, Horwitz JA, Donovan BM, Labitt RN, Budell WC, Friling T, Vogt A, Catanese MT, Satoh T, Kawai T, Akira S, Law M, Rice CM, Ploss A
    • Journal Title

      Nature

      Volume: 12;501(7466) Issue: 7466 Pages: 237-41

    • DOI

      10.1038/nature12427

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Poly IC Triggers a Cathepsin D- and IPS-1-Dependent Pathway to Enhance Cytokine Production and Mediate Dendritic Cell Necroprosis.2013

    • Author(s)
      Zou J, Kawai T, Tsuchida T, Kozaki T, Tanaka H, Shin KS, Kumar H, Akira S.
    • Journal Title

      Immunity.

      Volume: 38(49) Issue: 4 Pages: 717-728

    • DOI

      10.1016/j.immuni.2012.12.007

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage sensor MRE11 recognizes cytosolic double-stranded DNA and induces type I interferon by regulating STING traffickining2013

    • Author(s)
      Takeshi Kondo, Junya Kobayashi, Tatusya Saitoh, Kenta Maruyama. Ken J. Ishii, Glen N. Barber, Kenshi、Komatsu, Shizuo Akira, and Taro Kawai
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 110 Issue: 8 Pages: 2969-2974

    • DOI

      10.1073/pnas.1222694110

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Type-I interferon is critical for FasL expression on lung cells to determine the severity of influenza.2013

    • Author(s)
      Fujikura D, Chiba S, Muramatsu D, Kazumata M, Nakayama Y, Kawai T, Akira S, Kida H, Miyazaki T
    • Journal Title

      PLoS One

      Volume: 8(2) Issue: 2 Pages: e55321-e55321

    • DOI

      10.1371/journal.pone.0055321

    • NAID

      120005228345

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification2012

    • Author(s)
      Oldenburg M, Kruger A, Ferstl R, Kaufmann A, Nees G, Sigmund A, Bathke B, Lauterbach H, Suter M, Dreher S, Koedel U, Akira S, Kawai T, Buer J, Wagner H, Bauer S, Hochrein H, Kirschning CJ
    • Journal Title

      Science

      Volume: 31;337(6098) Issue: 6098 Pages: 1111-5

    • DOI

      10.1126/science.1220363

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissecting negative regulation of Toll-like receptor signaling.2012

    • Author(s)
      Kondo T
    • Journal Title

      Trends Immunol

      Volume: 33(9) Issue: 9 Pages: 449-458

    • DOI

      10.1016/j.it.2012.05.002

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IPS-1 signaling has a nonredundant role in mediating antiviral responses and the clearance of respiratory syncytial virus.2012

    • Author(s)
      Demoor T
    • Journal Title

      J Immunol.

      Volume: 189 Issue: 12 Pages: 5942-5953

    • DOI

      10.4049/jimmunol.1201763

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CD44 participates in IP-10 induction in cells in which hepatitis C virus RNA is replicating, through an interaction with Toll-like receptor 2 and hyaluronan.2012

    • Author(s)
      Abe T
    • Journal Title

      J Virol.

      Volume: 86 Issue: 11 Pages: 6159-6170

    • DOI

      10.1128/jvi.06872-11

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recognition of nucleic acids by pattern-recognition receptors and its relevance in autoimmunity2011

    • Author(s)
      Kawasaki T, Kawai T, Akira S
    • Journal Title

      Immunol Rev

      Volume: 243 Issue: 1 Pages: 61-73

    • DOI

      10.1111/j.1600-065x.2011.01048.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recognition of nucleic acids by pattern-recognition receptors and its relevance in autoimmunity2011

    • Author(s)
      Kawasaki T, et al
    • Journal Title

      Immunol Rev

      Volume: 243 Issue: 9 Pages: 61-73

    • DOI

      10.1002/emmm.201100160

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Viral infection augments Nod1/2 signaling to potentiate lethality associated with secondary bacterial infections2011

    • Author(s)
      Kim YG, Park JH, Reimer T, Baker DP, Kawai T, Kumar H, Akira S, Wobus C, Nunez G
    • Journal Title

      Cell Host Microbe

      Volume: 9 Pages: 496-507

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toll-like receptors and their crosstalk with other innate receptors in infection and immunity2011

    • Author(s)
      Kawai T, Akira S
    • Journal Title

      Immunity

      Volume: 34 Pages: 637-650

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4)2011

    • Author(s)
      Togi S, Ikeda O, Kamitani S, Nakasuji M, Sekine Y, Muromoto R, Nanbo A, Oritani K, Kawai T, Akira S, Matsuda T
    • Journal Title

      J Biol Chem

      Volume: 286 Pages: 19170-19177

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Role of RIG-I-like receptor signaling for mounting antiviral immunity2013

    • Author(s)
      Kawai T
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      千葉(シンポジウム)
    • Year and Date
      2013-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Poly ICのアジュバント効果における細胞死の役割2013

    • Author(s)
      河合太郎
    • Organizer
      第6回寄生虫感染免疫研究会
    • Place of Presentation
      大分
    • Year and Date
      2013-03-08
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Role of RIG-I-like receptor signaling for mounting antiviral immunity2013

    • Author(s)
      Taro Kawai
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      幕張メッセ、千葉
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Poly ICのアジュバント効果における細胞死の役割2013

    • Author(s)
      河合太郎
    • Organizer
      第6回 寄生虫感染免疫研究会
    • Place of Presentation
      大分
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 自然免疫受容体による病原体認識と生体防御における役割2012

    • Author(s)
      河合太郎
    • Organizer
      肝と免疫研究会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Dissection of signaling pathways through innate receptors that sense foreign nucleic acids2011

    • Author(s)
      河合太郎
    • Organizer
      第40回日本免疫学会学術集会(シンポジウムS-1)
    • Place of Presentation
      幕張メッセ(千葉)(招待講演)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Annual Research Report
  • [Book] パターン認識受容体(PRR)の展開. The Frontiers in Life Sciencesシリーズ免疫学Update2012

    • Author(s)
      國吉佳奈子、鍛代悠一、審良静男、河合太郎
    • Publisher
      南山堂
    • Related Report
      2013 Final Research Report
  • [Book] Poly I : Cの免疫アジュバント作用とその機序.臨床免疫・アレルギー科58(4).2012

    • Author(s)
      河合太郎
    • Publisher
      科学評論社
    • Related Report
      2013 Final Research Report
  • [Book] 免疫学Update2012

    • Author(s)
      鍛代悠一
    • Total Pages
      6
    • Publisher
      南山堂
    • Related Report
      2012 Annual Research Report
  • [Book] 臨床免疫・アレルギー科2012

    • Author(s)
      河合太郎
    • Total Pages
      6
    • Publisher
      科学評論社
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://bsw3.naist.jp/kawai/

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://hostdefense.ifrec.osaka-u.ac.jp/ja/index.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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