Develop the method to measure the autophagic activity

• Project/Area Number: 23689032
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Niigata University (2012-2013); Kyushu University (2011)
• Principal Investigator: KANKI Tomotake 新潟大学, 医歯学系, 教授 (50398088)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2011: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
• Keywords: 臨床検査医学 / オートファジー / 臨床検査

Research Abstract

Autophagy is a catabolic process that degrades cytoplasmic components and organelles, and is maintain cellular homeostasis by eliminating unnecessary cellular components. Thus, the reduction of autophagic activity relates several disorders. However, it is difficult to measure the autophagic activity of patients. The goal of this study is to establish the method to measure the autophagic activity of patients for clinical use. As an initial step, we tried to observe autophagy in cultured mammalian cells. To observe autophagy, we established pH-sensitive fluorescent protein Keima expressing cell lines (Keima cells). Using Keima cells, we succeeded to observe autophagy sensitively and semi-quantitatively.

Research Products

• [Journal Article] Casein kinase 2 is essential for mitophagy. (2013)
  • Author(s): Kanki T., Kurihara Y., Jin X., Goda T., Ono Y., Aihara M., Hirota Y., Saigusa T., Aoki Y., Uchiumi T., Kang D.
  • Journal Title: EMBO reports Volume: 14巻 Issue: 9 Pages: 788-794
  • DOI: 10.1038/embor.2013.114
  • Peer Reviewed
• [Journal Article] Effects of overexpression of mitochondrial transcription factor A on lifespan and oxidative stress response in Drosophila melanogaster (2013)
  • Author(s): Matsuda T, Kanki T, Tanimura T, Kang D, Matsuura ET
  • Journal Title: Biochem Biophys Res Commun Volume: 430(2) Issue: 2 Pages: 717-21
  • DOI: 10.1016/j.bbrc.2012.11.084
  • Peer Reviewed
• [Journal Article] Mutation and functional analysis of ABCC2/multidrug resistance protein 2 in a Japanese patient with Dubin-Johnson syndrome (2013)
  • Author(s): Uchiumi T, Tanamachi H, Kuchiwaki K, Kajita M, Matsumoto S, Yagi M, Kanki T, Kang D
  • Journal Title: Hepatol Res Volume: 43(5) Issue: 5 Pages: 569-75
  • DOI: 10.1111/j.1872-034x.2012.01103.x
  • NAID: 10031183491
  • Peer Reviewed
• [Journal Article] Mutation and functional analysis of ABCC2/multidrug resistance protein 2 in a Japanese patient with Dubin-Johnson syndrome. (2013)
  • Author(s): Uchiumi T
  • Journal Title: Hepatol Res. Volume: 43 Pages: 569-575
  • Peer Reviewed
• [Journal Article] Protein instability and functional defects caused by mutations of dihydro-orotate dehydrogenase in Miller syndrome patients. (2012)
  • Author(s): Fang J, Uchiumi T, Yagi M, Matsumoto S, Amamoto R, Saito T, Takazaki S, Kanki T, Yamaza H, Nonaka K, Kang D.
  • Journal Title: Biosci Rep Volume: 32 Issue: 6 Pages: 631-639
  • DOI: 10.1042/bsr20120046
  • Peer Reviewed
• [Journal Article] Guideline for the use and interpretation of assays for monitoring autophagy (2012)
  • Author(s): Klionsky DJ,et al
  • Journal Title: Autophagy Volume: 8 Issue: 4 Pages: 445-544
  • DOI: 10.4161/auto.19496
  • Peer Reviewed
• [Journal Article] p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA binding ability. (2012)
  • Author(s): Yagi M, Uchiumi T, Takazaki S, Okuno B, Nomura M, Yoshida S, Kanki T, Kang D
  • Journal Title: Nuc Acid Res Volume: 19 Issue: 19 Pages: 9717-37
  • DOI: 10.1093/nar/gks774
  • Peer Reviewed
• [Journal Article] Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins (2012)
  • Author(s): Matsumoto S, Uchiumi T, Saito T, Yagi M, Takazaki S, Kanki T, Kang D
  • Journal Title: Mitochondrion Volume: 12(3) Issue: 3 Pages: 391-8
  • DOI: 10.1016/j.mito.2012.02.004
  • Peer Reviewed
• [Journal Article] Ribonucleoprotein Y-box binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA (2012)
  • Author(s): Matsumoto S, Uchiumi T, et al
  • Journal Title: Biochemical J Volume: 443 Issue: 2 Pages: 573-584
  • DOI: 10.1042/bj20111728
  • Peer Reviewed
• [Journal Article] The physiological role of mitophagy: new insights into phosphorylation events. (2012)
  • Author(s): Hirota, Y., Kang, D., Kanki, T.
  • Journal Title: International journal of cell biology Volume: 2012 Pages: 354914-354914
  • DOI: 10.1155/2012/354914
  • Peer Reviewed
• [Journal Article] Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast (2012)
  • Author(s): Kurihara Y, Kanki T, Aoki Y, Hirota Y, Saigusa T, Uchiumi T, Kang D
  • Journal Title: J Biol Chem Volume: 287 Issue: 5 Pages: 3265-3272
  • DOI: 10.1074/jbc.m111.280156
  • Peer Reviewed
• [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy. (2012)
  • Author(s): Klionsky DJ
  • Journal Title: Autophagy. Volume: 8 Pages: 445-544
  • Peer Reviewed
• [Journal Article] Phosphorylation of Serine 114 on Atg32 mediates mitophagy (2011)
  • Author(s): Aoki, Y., Kanki, T., Hirota, Y., Kurihara, Y., Saigusa, T., Uchiumi, T., Kang, D.
  • Journal Title: Mol Biol Cell Volume: 22 Issue: 17 Pages: 3206-3217
  • DOI: 10.1091/mbc.e11-02-0145
  • Peer Reviewed
• [Journal Article] Mitochondria autophagy in yeast (2011)
  • Author(s): Kanki, T., et al
  • Journal Title: Antioxidants and Redox Signaling Volume: 14 Issue: 10 Pages: 1989-2001
  • DOI: 10.1089/ars.2010.3762
  • Peer Reviewed
• [Presentation] Signaling pathways regulating mitochondria autophagy in yeast (2013)
  • Author(s): Kanki T
  • Organizer: ASMRM2013
  • Place of Presentation: 韓国ソウル
  • Year and Date: 2013-11-04
• [Presentation] Mitophagy: selective degradation of mitochondria via autophagy (2013)
  • Author(s): Kanki T.
  • Organizer: THE FRONTIER OF MITOCHONDRIAL RESEARCH
  • Place of Presentation: 山形
  • Invited
• [Presentation] 出芽酵母におけるミトコンドリアオートファジーの制御機構 (2013)
  • Author(s): 神吉智丈
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] ミトコンドリアオートファジーの分子機構と生理的意義 (2012)
  • Author(s): 神吉智丈
  • Organizer: 第363回日本泌尿器科学会新潟地方会
  • Place of Presentation: 長岡
  • Year and Date: 2012-09-08
• [Presentation] ミトコンドリアオートファジー (2012)
  • Author(s): 神吉智丈
  • Organizer: 第193回岡山腹腔鏡研究会
  • Place of Presentation: 岡山(招待講演)
  • Year and Date: 2012-03-31
• [Presentation] Physiological role of mitophagy in yeast (2012)
  • Author(s): Kanki T., et la
  • Organizer: Gordon research conference
  • Place of Presentation: Ventura, USA
  • Year and Date: 2012-03-11
• [Presentation] 出芽酵母におけるマイトファジーのシグナル経路 (2012)
  • Author(s): 神吉智丈
  • Organizer: 日本ミトコンドリア学会 第12回年会
  • Place of Presentation: 筑波大学 大学会館(つくば市）
• [Presentation] 出芽酵母におけるマイトファジーの分子機構と生理的意義 (2011)
  • Author(s): 神吉智丈
  • Organizer: タンパク質の社会全体班会議
  • Place of Presentation: 別府
  • Year and Date: 2011-11-21
• [Book] Assays for Autophagy II : Mitochondrial Autophagy Wei Xiao (ed.), Yeast Protocols, Methods in Molecular Biology (2014)
  • Author(s): Kanki T and Okamoto K
  • Publisher: (doi:10.1007/978-1-4939-0799-1_11)
• [Book] Yeast Protocols, Third Edition (2014)
  • Author(s): Kanki T, Okamoto K
  • Publisher: Humana Press
• [Book] AUTOPHAGY, CANCER, OTHER PATHOLOGIES, INFLAMMATION, IMMUNITY, INFECTION AND AGING, VOLUME 4 (2014)
  • Author(s): Kukrihara U, Kanki T.
  • Publisher: Elsevier Inc.
• [Book] ATG32 CONFERS SELECTIVE MITOCHONDRIAL SEQUESTRATION AS A CARGO FOR AUTOPHAGY AUTOPHAGY, CANCER, OTHER PATHOLOGIES, INFLAMMATION, IMMUNITY, INFECTION AND AGING, VOLUME 4: MITOPHAGY
  • Author(s): Kurihara Y and Kanki T
  • Publisher: (印刷中)
• [Remarks]
  • URL: http://www.niigata-u.ac.jp/tenure_track/researcher/kanki_tomotake.html
• [Remarks] 新潟大学 自立・競争的環境で育てる若手研究者育成プログラム
  • URL: http://www.niigata-u.ac.jp/tenure_track/researcher/kanki_tomotake.html
• [Remarks] 新潟大学 若手研究者育成プログラム
  • URL: http://www.niigata-u.ac.jp/tenure_track/researcher/kanki_tomotake.html