Research Project
Grant-in-Aid for Young Scientists (A)
Polycomb group protein EZH2 is essential for the self-renewal of liver cancer stem cells. Short hairpin-RNA against EZH2 and 3-Deazaneplanocin A (DZNep) promotes the conversion toward differentiated progeny cells and reduced tumorigenic ability thorough the inhibition of H3K27 trimethylation (H3K27me3). We also conducted chromatin immunoprecipitation sequencing (ChIP-seq) analysis of normal hepatic stem cells using anti-H3K27me3 and anti-H3K4me3 antibodies, and successfully identified 562 bivalent genes. In combination with microarray data in the differentiation induction, candidate genes closely associated with hepatocytes and/or cholangiocytes were selected from bivalent genes. Since cancer-related genes were included among these genes, further analyses would be necessary to examine the role of these molecules in normal hepatic stem cells and liver cancer stem cells.
All 2014 2013 2012 2011 Other
All Journal Article (18 results) (of which Peer Reviewed: 18 results, Open Access: 1 results) Presentation (17 results) Patent(Industrial Property Rights) (3 results)
PLoS One
Volume: 9(1) Issue: 1 Pages: e84807-e84807
10.1371/journal.pone.0084807
Stem Cell Reports.
Volume: 2 Issue: 2 Pages: 145-52
10.1016/j.stemcr.2013.12.008
J Gastroenterol Hepatol
Volume: 29(1) Issue: 1 Pages: 165-72
10.1111/jgh.12449
Int. J. Oncol.
Volume: 43 Issue: 6 Pages: 1846-1854
10.3892/ijo.2013.2120
Anticancer Res
Volume: 33(12) Pages: 5603-9
PloS one
Volume: 8 Issue: 7 Pages: e70010-e70010
10.1371/journal.pone.0070010
Magn Reson Imaging
Volume: 31(5) Issue: 5 Pages: 748-54
10.1016/j.mri.2012.10.028
Hepatobiliary Surg Nutr
Volume: 2(1) Pages: 4-6
Int J Med Sci
Volume: 10(5) Issue: 5 Pages: 567-74
10.7150/ijms.5795
Hepatobiliary Surg Nutr.
Volume: 2 Pages: 4-6
Hepatol Res
Volume: 42(11) Issue: 11 Pages: 1100-11
10.1111/j.1872-034x.2012.01028.x
Blood
Volume: 120 Issue: 5 Pages: 1107-17
10.1182/blood-2011-11-394932
Oncology Reports
Volume: 28(1) Pages: 8-14
10.3892/or.2012.1763
International Journal of Cancer
Volume: 130(11) Issue: 11 Pages: 2557-2567
10.1002/ijc.26264
Int J Cancer
Volume: 130 Pages: 2557-67
Volume: (In press)
Oncol Rep
Volume: 118 Pages: 2443-53