Epigenetic regulation of liver cancer stem cells and establishment of a novel therapeutic approach
Project/Area Number |
23689039
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
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Research Institution | Chiba University |
Principal Investigator |
CHIBA Tetsuhiro 千葉大学, 医学(系)研究科(研究院), 講師 (00381583)
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Project Period (FY) |
2011-04-01 – 2013-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2012: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2011: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
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Keywords | エピジェネティクス / 肝癌 / 肝臓 / 幹細胞 / 肝臓癌 / 癌幹細胞 / ポリコーム遺伝子 / DZNep / 癌幹細胞特異的治療法 |
Research Abstract |
Polycomb group protein EZH2 is essential for the self-renewal of liver cancer stem cells. Short hairpin-RNA against EZH2 and 3-Deazaneplanocin A (DZNep) promotes the conversion toward differentiated progeny cells and reduced tumorigenic ability thorough the inhibition of H3K27 trimethylation (H3K27me3). We also conducted chromatin immunoprecipitation sequencing (ChIP-seq) analysis of normal hepatic stem cells using anti-H3K27me3 and anti-H3K4me3 antibodies, and successfully identified 562 bivalent genes. In combination with microarray data in the differentiation induction, candidate genes closely associated with hepatocytes and/or cholangiocytes were selected from bivalent genes. Since cancer-related genes were included among these genes, further analyses would be necessary to examine the role of these molecules in normal hepatic stem cells and liver cancer stem cells.
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Report
(3 results)
Research Products
(38 results)
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[Journal Article] Disulfiram eradicates tumor-initiating hepatocellular carcinoma cells in ROS-p38 MAPK pathway-dependent and -independent manners2014
Author(s)
Chiba T, Suzuki E, Yuki K, Zen Y, Oshima M, Miyagi S, Saraya A, Koide S, Motoyama T, Ogasawara S, Ooka Y, Tawada A, Nakatsura T, Hayashi T, Yamashita T, Kaneko S, Miyazaki M, Iwama A, Yokosuka O
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Journal Title
PLoS One
Volume: 9(1)
Issue: 1
Pages: e84807-e84807
DOI
Related Report
Peer Reviewed
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[Journal Article] The TIF1β-HP1 System Maintains Transcriptional Integrity of Hematopoietic Stem Cells.2014
Author(s)
Miyagi S, Koide S, Saraya A, Wendt GR, Oshima M, Konuma T, Yamazaki S, Mochizuki-Kashio M, Nakajima-Takagi Y, Wang C, Chiba T, Kitabayashi I, Nakauchi H, Iwama A.
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Journal Title
Stem Cell Reports.
Volume: 2
Issue: 2
Pages: 145-52
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effect of metformin on residual cells after chemotherapy in a human lung adenocarcinoma cell line.2013
Author(s)
Kitazono, S., Takiguchi, Y., Ashinuma, H., Saito-Kitazono, M., Kitamura, A., Chiba, T., Sakaida, E., Sekine, I., Tada, Y., Kurosu, K., Sakao, S., Tanabe, N., Iwama, A., Yokosuka, O. and Tatsumi, K.
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Journal Title
Int. J. Oncol.
Volume: 43
Issue: 6
Pages: 1846-1854
DOI
Related Report
Peer Reviewed
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[Journal Article] Efficacy of transcatheter arterial chemoembolization with miriplatin-lipiodol water-soluble contrast agent emulsion in patients with hepatocellular carcinoma2013
Author(s)
Okimoto K, Ogasawara S, Chiba T, Ooka Y, Oobu M, Azemoto R, Kanogawa N, Motoyama T, Suzuki E, Tawada A, Yoshikawa M, Yokosuka O
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Journal Title
Anticancer Res
Volume: 33(12)
Pages: 5603-9
Related Report
Peer Reviewed
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[Journal Article] Metformin, a Diabetes Drug, Eliminates Tumor-Initiating Hepatocellular Carcinoma Cells.2013
Author(s)
Saito, T., Chiba, T., Yuki, K., Zen, Y., Oshima, M., Koide, S., Motoyama, T., Ogasawara, S., Suzuki, E., Ooka, Y., Tawada, A., Tada, M., Kanai, F., Takiguchi, Y., Iwama, A. and Yokosuka, O.
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Journal Title
PloS one
Volume: 8
Issue: 7
Pages: e70010-e70010
DOI
Related Report
Peer Reviewed
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[Journal Article] Adherence to medication is a more important contributor to viral breakthrough in chronic hepatitis B patients treated with entecavir than in those with Lamivudine2013
Author(s)
Kamezaki H, Kanda T, Arai M, Wu S, Nakamoto S, Chiba T, Maruyama H, Fujiwara K, Kanai F, Imazeki F, Nomura F, Yokosuka O
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Journal Title
Int J Med Sci
Volume: 10(5)
Issue: 5
Pages: 567-74
DOI
Related Report
Peer Reviewed
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[Journal Article] 3-Deazaneplanocin A is a promising therapeutic agent for the eradication of tumor-initiating hepatocellular carcinoma cells2012
Author(s)
Chiba T, Suzuki E, Negishi M, Saraya A, Miyagi S, Konuma T, Tanaka S, Tada M, Kanai F, Imazeki F, Iwama A, Yokosuka O
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Journal Title
International Journal of Cancer
Volume: 130(11)
Issue: 11
Pages: 2557-2567
DOI
Related Report
Peer Reviewed
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[Patent(Industrial Property Rights)] 肝がん幹細胞阻害剤2012
Inventor(s)
千葉哲博、他3名
Industrial Property Rights Holder
国立大学法人千葉大学
Industrial Property Rights Type
特許
Industrial Property Number
2012-224722
Filing Date
2012-10-10
Acquisition Date
2013-05-23
Related Report
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