Establishment of human liver chimeric mice with normal immune system for hepatitis viral infection
| Project/Area Number |
23689040
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Tokai University |
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Principal Investigator |
KAMIYA Akihide 東海大学, 創造科学技術研究機構, 准教授 (30321904)
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| Co-Investigator(Renkei-kenkyūsha) |
KAKINUMA Sei 東京医科歯科大学, 分子肝炎制御学講座, 特任講師 (30372444)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 肝炎ウイルス / 肝幹細胞 / 多能性幹細胞 / iPS細胞 / 肝前駆細胞 / 肝細胞生物学 / 肝幹・前駆細胞 |
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| Research Abstract |
Viral hepatitis is a very severe liver disease. Studies for therapies of this disease are difficult because Hepatitis viruses cannot infect to normal experimental animals such as mouse and rat. Thus, we have a plan to establish a new mouse model for the studies of viral hepatitis.
(1) We established a new culture method for the purification and expansion of hepatic progenitor cells derived from human pluripotent cells. These cells are candidate of donor cells into human liver chimera mice.
(2) We tried to transplant primary hepatic progenitor cells derived into neonatal mice. Small numbers of donor cells were detected in the recipient liver and lung.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] SALL4, a stem cell biomarker in liver cancers2013
Author(s)
Oikawa T, Kamiya A, Zeniya M, Chikada H, Hyuck AD, Yamazaki Y, Wauthier E, Tajiri H, Miller LD, Wang XW, Reid LM, Nakauchi H
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Journal Title
Hepatology
Volume: 57
Pages: 1469-83
Related Report
Peer Reviewed
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[Journal Article] Wnt5a Signaling Mediates Biliary Differentiation of Fetal Hepatic Stem/Progenitor Cells2013
Author(s)
Kakinuma S, Kiyohashi K, Kamiya A, Sakamoto N, Nitta S, Yamanaka H, YoshinoK, Fujiki J, Murakawa M, Kusano-Kitazume A, Shimizu H, Okamoto R, Azuma S, Nakagawa M, Asahina Y, Tanimizu N, Kikuchi A, Nakauchi H, and *WatanabeM
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Journal Title
Hepatology
Volume: (in press)
Issue: 6
Pages: 2502-2513
DOI
Related Report
Peer Reviewed
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[Journal Article] Sal-like protein 4 (SALL4), a stem cell biomarker in liver cancers.2012
Author(s)
Oikawa T, Kamiya A*, Zeniya M, Chikada H, Hyuck AD, Yamazaki Y, Wauthier E, Tajiri H, Miller LD, Wang XW, Reid LM*, Nakauchi H*.
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Journal Title
Hepatology
Volume: in press
Issue: 4
Pages: 1469-83
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of an All-in-One Inducible Lentiviral Vector for Gene Specific Analysis of Reprogramming.2012
Author(s)
Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H.
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Journal Title
PLoS One.
Volume: 7
Issue: 7
Pages: e41007-e41007
DOI
Related Report
Peer Reviewed
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