Establishment of cardiac regeneration therapy using modified reprogramming strategy and bioluminescent imaging
| Project/Area Number |
23689041
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Ritsumeikan University (2013-2014)
Kyoto University (2011-2012) |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | 再生医学 / 幹細胞生物学 / 体細胞初期化 |
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| Outline of Final Research Achievements |
Although induced pluripotent stem (iPS) cell-based cardiac regeneration therapy has been expected, safety, costs, and duration of iPS cell derivation are considered as barriers against the clinical application. To overcome these problems, we tried to modify the reprograming process, in order to create cardiac myocytes directly from fibroblasts rapidly and efficiently with few risks of tumorigenesis. Sca1+/CD34+ cells appeared in the early phase of four factor-mediated reprogramming of mouse embryonic fibroblasts. While these cells are not susceptible to iPS cell formation, they can be maintained during over 10 passages under a certain culture condition, and possess the ability to differentiate into cardiac myocytes. In addition, using bioluminescent imaging, these cells did not develop the teratoma formation after implantation into immune-deficient mice. Taken together, these cells could be a novel cell source for safe and efficient cardiac regeneration therapy.
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] MicroRNA-27a regulates beta cardiac myosin heavy chain gene expression by targeting thyroid hormone receptor betal in neonatal rat ventricular myocytes2011
Author(s)
Nishi H, Ono K, Horie T, Nagao K, Kinoshita M, Kuwabara Y, Watanabe S, Takaya T, Tamaki Y, Takanabe-Mori R, Wada H, Hasegawa K, Iwanaga Y, Kawamura I, Kita T, Kimura T
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Journal Title
Mol Cell Biol
Volume: 31
Pages: 744-755
Related Report
Peer Reviewed
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