Development of diagnostic technologies for ALK-positive lung cancer

• Project/Area Number: 23689043
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Jichi Medical University
• Principal Investigator: SODA Manabu 自治医科大学, 医学部, ポストドクター (10406118)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: EML4-ALK / 肺がん / multiplex RT-PCR法 / RT-PCR

Research Abstract

EML4-ALK and KIF5B-ALK lung cancer oncogenes have various fusion variants. We thus developed a multiplex RT-PCR system to capture all possible in-frame fusions for these oncogenes, and conducted a large-scale, prospective screening for Japanese cohort of

Research Products

• [Journal Article] Transforming mutations of RAC guanosine triphosphatases in human cancers (2013)
  • Author(s): Kawazu M, Ueno T, Kontani K, Ogita Y, Ando M, Fukumura K, Yamato A, Soda M, Takeuchi K, Miki Y, Yamaguchi H, Yasuda T, Naoe T, Yamashita Y, Katada T, Choi YL, & Mano H
  • Journal Title: PNAS. Volume: 110 Pages: 3029-334
• [Journal Article] (2013)
  • Author(s): Kawazu M, Ueno T, Kontani K, Ogita Y, Ando M, Fukumura K, Yamato A, Soda M, Takeuchi K, Miki Y, Yamaguchi H, Yasuda T, Naoe T,Yamashita Y, Katada T, Choi YL, Mano H,
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Issue: 8 Pages: 3029-3034
  • DOI: 10.1073/pnas.1216141110
  • Peer Reviewed
• [Journal Article] Oncogenic MAP2K1 mutations in human epithelial tumors (2012)
  • Author(s): Choi YL, Soda M, Ueno T, Hamada T, Haruta H, Yamato A, Fukumura K, Ando M, Kawazu M, Yamashita Y & Mano H
  • Journal Title: Carcinogenesis. Volume: 33 Pages: 956-961
• [Journal Article] ALK fusion gene positive lung cancer and 3 cases treated with an inhibitor for ALK kinase activity (2012)
  • Author(s): Kimura H, Nakajima T, Takeuchi K, Soda M, Mano H, Iizasa T, Matsui Y, Yoshino M, Shingyoji M, Itakura M, Itami M, Ikebe D, Yokoi S, Kageyama H, Ohira M & Nakagawara A
  • Journal Title: Lung Cancer Volume: 75 Pages: 66-72
• [Journal Article] A common BIM deletion polymorphism mediates intrinsic resistance and inferior responses to tyrosine kinase inhibitors in cancer (2012)
  • Author(s): Ng KP, Hillmer AM, Chuah CT, Juan WC, Ko TK, Teo AS, Ariyaratne PN, Takahashi N, Sawada K, Fei Y, Soh S, Lee WH, Huang JW, Allen JC, Jr., Woo XY, Nagarajan N, Kumar V, Thalamuthu A, Poh WT, Ang AL, Mya HT, How GF, Yang LY, Koh LP, Chowbay B, Chang CT, Nad
  • Journal Title: Nat Med Volume: 18 Pages: 521-528
• [Journal Article] A prospective PCR-based screening for the EML4-ALK oncogene in non-small cell lung cancer (2012)
  • Author(s): Soda M, Isobe K, Inoue A, Maemondo M, Oizumi S, Fujita Y, Gemma A, Yamashita Y, Ueno T, Takeuchi K, Choi YL, Miyazawa H, Tanaka T, Hagiwara K & Mano H
  • Journal Title: Clin Cancer Res Volume: 18 Pages: 5682-5689
• [Journal Article] RET, ROS1 and ALK fusions in lung cancer (2012)
  • Author(s): Takeuchi K, Soda M, Togashi Y, Suzuki R, Sakata S, Hatano S, Asaka R, Hamanaka W, Ninomiya H, Uehara H, Choi YL, Satoh Y, Okumura S, Nakagawa K, Mano H & Ishikawa Y
  • Journal Title: Nat Med Volume: 18 Pages: 378-381
• [Journal Article] KLC1-ALK: a novel fusion in lung cancer identified using a formalin-fixed paraffin-embedded tissue only (2012)
  • Author(s): Togashi Y, et al.
  • Journal Title: PLoS One Volume: 7 Issue: 2 Pages: 31323-31323
  • DOI: 10.1371/journal.pone.0031323
  • Peer Reviewed
• [Journal Article] High-throughput resequencing of target-captured cDNA in cancer cells (2012)
  • Author(s): Ueno T, Yamashita Y, Soda M, Fukumura K, Ando M, Yamato A, Kawazu M, Choi YL & Mano H
  • Journal Title: Cancer Sci Volume: 103 Pages: 131-135
• [Journal Article] Paracrine Receptor Activation by Microenvironment Triggers Bypass Survival Signals and ALK Inhibitor Resistance in EML4-ALK Lung Cancer Cells (2012)
  • Author(s): Yamada T, Takeuchi S, Nakade J, Kita K, Nakagawa T, Nanjo S, Nakamura T, Matsumoto K, Soda M, Mano H, Uenaka T & Yano S
  • Journal Title: Clin Cancer Res Volume: 18 Pages: 3592-3602
• [Journal Article] (2012)
  • Author(s): Soda M, Isobe K, Inoue A, Maemondo M,Oizumi S, Fujita Y, Gemma A, Yamashita Y,Ueno T, Takeuchi K, Choi YL, Miyazawa H,Tanaka T, Hagiwara K, Mano H
  • Journal Title: Clin Cancer Res Volume: 18 Issue: 20 Pages: 5682-5689
  • DOI: 10.1158/1078-0432.ccr-11-2947
  • Peer Reviewed
• [Journal Article] Oncogenic MAP2K1 mutations in human epithelial tumors (2012)
  • Author(s): Choi, Y. L., Soda, M., et al.(全11名、第10著者)
  • Journal Title: Carcinogenesis Volume: 33 Issue: 5 Pages: 956-961
  • DOI: 10.1093/carcin/bgs099
  • Peer Reviewed
• [Journal Article] Paracrine receptor activation by microenvironment triggers bypass survival signals and ALK inhibitor resistance in EML4-ALK lung cancer cells (2012)
  • Author(s): Yamada, T.
  • Journal Title: Clin. Cancer Res. Volume: 18 Issue: 13 Pages: 3592-3602
  • DOI: 10.1158/1078-0432.ccr-11-2972
  • Peer Reviewed
• [Journal Article] Pulmonary inflammatory myofibroblastic tumor expressing a novel fusion, PPFIBP1-ALK: reappraisal of anti-ALK immunohistochemistry as a tool for novel ALK-fusion identification" (2011)
  • Author(s): Takeuchi K, Soda M, Togashi Y, Sugawara E, Hatano S, Asaka R, Okumura S, Nakagawa K, Mano H, Ishikawa Y
  • Journal Title: Clin Cancer Res Volume: 17 Pages: 3341-3348
• [Journal Article] Identification of a novel fusion, SQSTM1-ALK, in ALK-positive large B-cell lymphoma (2011)
  • Author(s): Takeuchi K, et al
  • Journal Title: Haematologica Volume: 96 Issue: 3 Pages: 464-467
  • DOI: 10.3324/haematol.2010.033514
  • NAID: 40019402944
  • Peer Reviewed
• [Journal Article] Pulmonary inflammatory myofibroblastic tumor expressing a novel fusion, PPFIBP1-ALK: reappraisal of anti-ALK immunohistochemistry as a tool for novel ALK fusion identification (2011)
  • Author(s): Takeuchi K, et al
  • Journal Title: Clinical Cancer Research Volume: 17 Issue: 10 Pages: 3341-3348
  • DOI: 10.1158/1078-0432.ccr-11-0063
  • Peer Reviewed
• [Presentation] ALK肺がんの発見から治療まで (2013)
  • Author(s): 曽田学
  • Organizer: 日本がん分子標的治療学会第8回TRWS
  • Place of Presentation: 東京
  • Year and Date: 2013-01-22
• [Presentation] Clinical application of EML4-ALK lung cancer oncogene (2013)
  • Author(s): Manabu Soda, Kengo Takeuchi, Young Lim Choi, Toshihide Ueno, Yoshihiro Yamashita, Hiroyuki Mano
  • Organizer: 9th AACR-JCA Joint Conference
  • Place of Presentation: アメリカ、ハワイ
• [Presentation] Clinical application of EML4-ALK lung cancer oncogene (2013)
  • Author(s): Soda, M.
  • Organizer: 9th AACR-JCA Joint Conference
  • Place of Presentation: 米国、ハワイ
• [Presentation] EML4-ALK陽性肺がん:発見から臨床応用まで (2012)
  • Author(s): 曽田学
  • Organizer: 第53回日本肺癌学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2012-11-08
• [Presentation] Targeting ALK-fusions in lung cancer (2012)
  • Author(s): 曽田学
  • Organizer: 第71回日本癌学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-19
• [Presentation] Targeting ALK in lung cancer (2012)
  • Author(s): 曽田学
  • Organizer: 第10回日本臨床腫瘍学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-07-27
• [Presentation] 肺がんの新たな分子標的 (2012)
  • Author(s): 曽田学
  • Organizer: 第38回肺癌診断会および画像診断セミナー(招待講演)、ALK融合型チロシンキナーゼ
  • Place of Presentation: 新潟
  • Year and Date: 2012-06-23
• [Presentation] 標的分子内変異による薬剤耐性 (2012)
  • Author(s): 曽田 学
  • Organizer: 第16回日本がん分子標的治療学会学術集会
  • Place of Presentation: 小倉
• [Presentation] ALK融合型チロシンキナーゼ (2012)
  • Author(s): 曽田 学
  • Organizer: 第38回肺癌診断会および画像診断セミナー
  • Place of Presentation: 新潟
• [Presentation] EML4-ALK陽性肺がん：発見から臨床応用まで (2012)
  • Author(s): 曽田 学
  • Organizer: 第53回日本肺癌学会総会
  • Place of Presentation: 岡山
• [Presentation] Discovery of EML4-ALK oncogene: an effective target in lung cancer (2011)
  • Author(s): Manabu Soda
  • Organizer: 1st Korea-Japan Joint Lung Cancer Symposium (招待講演)
  • Place of Presentation: 韓国、ソウル
  • Year and Date: 2011-11-18
• [Presentation] Discovery of EML4-ALK oncogene : an effective target in lung cancer (2011)
  • Author(s): 曽田学
  • Organizer: 1^<st> Korea-Japan Joint Lung Cancer Symposium
  • Place of Presentation: 韓国、ソウル(招待講演)
  • Year and Date: 2011-11-18
• [Presentation] Discovery of ALK mutation and its clinical application (2011)
  • Author(s): 曽田学
  • Organizer: 第70回日本癌学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] Discovery of ALK mutation and its clinical application (2011)
  • Author(s): 曽田学
  • Organizer: 第9回日本臨床腫瘍学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-07-22
• [Presentation] ALK融合型チロシンキナーゼを標的とした分子診断法・分子標的療法の開発 (2011)
  • Author(s): 曽田学
  • Organizer: 第26回日本肺癌学会ワークショップ
  • Place of Presentation: 名古屋
  • Year and Date: 2011-07-16
• [Presentation] ALK融合型チロシンキナーゼを標的とした分子診断法・分子標的療法の開発 (2011)
  • Author(s): 曽田学
  • Organizer: 第66回呼吸器合同北陸地方会(招待講演)
  • Place of Presentation: 新潟
  • Year and Date: 2011-06-11
• [Presentation] EML4-ALK融合型チロシンキナーゼを標的とした肺がんの分子診断法・分子標的療法の開発 (2011)
  • Author(s): 曽田学
  • Organizer: 第66回呼吸器合同北陸地方会
  • Place of Presentation: 新潟(招待講演)
  • Year and Date: 2011-06-11
• [Presentation] Discovery of EML4-ALK fusion oncogene; an effective target in lung cancer (2011)
  • Author(s): Manabu Soda, Toshihide Ueno, Yoshihiro Yamashita, Hiroyuki Mano
  • Organizer: 2012 COLD SPRING HARBOR ASIA CONFERENCES
  • Place of Presentation: 中国、蘇州
  • Year and Date: 2011-05-25
• [Presentation] Discovery of EML4-ALK fusion oncogene-An effective target in lung cancer (2011)
  • Author(s): 曽田学
  • Organizer: COLD SPRING HARBOR ASIA CONFERENCES
  • Place of Presentation: 中国、蘇州
  • Year and Date: 2011-05-25
• [Presentation] Current status and role of ALK mutational testing in NSCLC (2011)
  • Author(s): 曽田学
  • Organizer: ATS 2011 International Conference
  • Place of Presentation: アメリカ、デンバー(招待講演)
  • Year and Date: 2011-05-13
• [Presentation] Current status and role of ALK mutational testing in NSCLC (2011)
  • Author(s): Manabu Soda
  • Organizer: ATS 2011 International Conference (招待講演)
  • Place of Presentation: アメリカ、デンバー
  • Year and Date: 2011-05-01
• [Presentation] 標的分子内変異による薬剤耐性 (2011)
  • Author(s): 曽田学
  • Organizer: 第16回日本がん分子標的治療学会学術集会
  • Place of Presentation: 小倉
• [Book] 成人病と生活習慣病 (2012)
  • Author(s): 曽田学, 他
  • Publisher: 東京医学社
• [Book] 医学のあゆみ (2012)
  • Author(s): 曽田学, 他
  • Publisher: 医歯薬出版株式会社
• [Book] 医学のあゆみ (2011)
  • Author(s): 曽田学, 他
  • Publisher: 医歯薬出版株式会社