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Development of cell therapy for epidermolysis bullosa

Research Project

Project/Area Number 23689053
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionHokkaido University

Principal Investigator

FUJITA Yasuyuki  北海道大学, 北海道大学病院, 助教 (80374437)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2011: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
KeywordsXVII型コラーゲン / 造血幹細胞移植 / 間葉系間質細胞 / 経骨髄移植 / 表皮水疱症 / 幹細胞移植 / VII型コラーゲン
Research Abstract

We performed stem cell transplantation including bone marrow transplantation and mesenchymal stem/stromal cell infusion into adult epidermolysis bullosa model mice (type XVII collagen (COL17) knockout mice). 1. Intramedullary infusion/transplantation induced better engraftment in the peripheral blood than conventional intravascular infusion, and the recovery of COL17 was also observed as well. 2. Donor-derived keratinocytes and recipient-derived keratinocytes were respectively collected by flowcytometer, and investigated mRNA expression differences by microarray technique. 57 genes were detected which have different mRNA expression between the two groups. 3. In corporation with Nagoya University, clinical trials of stem cell transplantation for severe epidermolysis bullosa patients are now applying, and it is under consideration.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • Research Products

    (14 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (7 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] Long-term Follow-up of Cultured Epidermal Autograft in a Patient with Recessive Dystrophic Epidermolysis Bullosa.2013

    • Author(s)
      Shinkuma S, Fujita Y, et al
    • Journal Title

      Acta Dermato-Venereologica

      Volume: 印刷中

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Method for Intravital Monitoring of Human Cells Using a Far-Red Luminescent Probe in Graft-Versus-Host Disease Model Mice.2013

    • Author(s)
      Qiao H, Fujita Y, et al
    • Journal Title

      Journal of Investigative Dermatology

      Volume: 印刷中 Issue: 6 Pages: 1692-1692

    • DOI

      10.1038/jid.2013.81

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conversion from human haematopoietic stem cells to keratinocytes requires keratinocyte secretory factors.2012

    • Author(s)
      Fujita Y, Inokuma D, Abe R, Sasaki M, Nakamura H, Shimizu T, Shimizu H:
    • Journal Title

      Clin Exp Dermatol

      Volume: 37 Pages: 658-664

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conversion from human haematopoietic stem cells to keratinocytes requires keratinocyte secretory factors2012

    • Author(s)
      Fujita Y, Inokuma D, et al.
    • Journal Title

      Clinical and Experimental Dermatology

      Volume: (印刷中) Issue: 6 Pages: 658-664

    • DOI

      10.1111/j.1365-2230.2011.04312.x

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regenerative medicine for severe congenital skin disorders: restoration of deficient skin component proteins by stem cell therapy.2011

    • Author(s)
      Fujita Y, Abe R, Nishie W, Shimizu H:
    • Journal Title

      Inflammation and Regeneration

      Volume: 31 Pages: 282-289

    • NAID

      130004943796

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regenerative medicine for severe congenital skin disorders : restoration of deficient skin component proteins by stem cell therapy2011

    • Author(s)
      Fujita Y, Abe R, et al
    • Journal Title

      Inflammation and Regeneration

      Volume: 31 Pages: 282-289

    • NAID

      130004943796

    • Related Report
      2011 Annual Research Report
  • [Presentation] Circulating stem cells and wounded skin in epidermolysis bullosa ? possible role of chemokines in the recruitment of cells from bone marrow to skin.2012

    • Author(s)
      Fujita Y, Abe R, Hayashi-Ujiie I, Shimizu H
    • Organizer
      The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
    • Place of Presentation
      LOISIR HOTEL NAHA(Okinawa)
    • Year and Date
      2012-12-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 表皮水疱症の病態と治療2012

    • Author(s)
      藤田靖幸
    • Organizer
      第76回日本皮膚科学会東部支部学術大会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Year and Date
      2012-09-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.2012

    • Author(s)
      藤田靖幸
    • Organizer
      第111回日本皮膚科学会総会(皆見省吾記念賞受賞講演)
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2012-06-04
    • Related Report
      2012 Final Research Report
  • [Presentation] Circulating stem cells and wounded skin in epidermolysis bullosa – possible role of chemokines in the recruitment of cells from bone marrow to skin.2012

    • Author(s)
      Fujita Y
    • Organizer
      The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
    • Place of Presentation
      ロワジールホテル那覇(沖縄県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.2012

    • Author(s)
      藤田靖幸
    • Organizer
      第111回日本皮膚科学会総会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 表皮水疱症の病態と治療2012

    • Author(s)
      藤田靖幸
    • Organizer
      第76回日本皮膚科学会東部支部学術大会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 骨髄移植は表皮水疱症モデルマウスに対する治療法となる2011

    • Author(s)
      藤田靖幸,阿部理一郎,猪熊大輔,佐々木美香子,守屋歩,西江渉,清水宏
    • Organizer
      第387回日本皮膚科学会北海道地方会
    • Place of Presentation
      大正製薬ビル(札幌市)
    • Year and Date
      2011-09-03
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Remarks] 北海道大学医学部皮膚科

    • URL

      http://www.derm-hokudai.jp/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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