Comprehensive analysis of LINE-1 hypomethylation level aimed at the development of new treatments for esophageal cancer
| Project/Area Number |
23689061
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
BABA Yoshifumi 熊本大学, 大学院生命科学研究部, 講師 (20599708)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2011: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Keywords | LINE-1 / メチル化 / 予後因子 / エピジェネティクス / 食道癌 / Pyrosequence / pyrosequence |
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| Research Abstract |
Using more than 200 ESCC specimens,we quantified the LINE-1 methylation by utilizing the bisulfite pyrosequencing technology. LINE-1 hypomethylation in ESCC is associated with a shorter survival, thus suggesting that it has potential for use as a prognostic biomarker. Result of CGH array, LINE-1-hypomethylated tumors showed highly frequent genomic gains at various loci containing candidate oncogenes such as CDK6. In our cohort of 129 patients with ESCC, cases with CDK6-positive expression experienced worse clinical outcome compared with those with CDK6-negative expression, supporting the oncogenic role of CDK6 in ESCC. In addition, we found that the prognostic impact of LINE-1 hypomethylation might be attenuated by CDK6 expression. LINE-1 hypomethylation in ESCC might contribute to the acquisition of aggressive tumor behavior through genomic gains of oncogenes such as CDK6.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] LINE-1 Hypomethylation, DNA Copy Number Alterations, and CDK6 Amplification in Esophageal Squamous Cell Carcinoma.2014
Author(s)
Y. Baba, M. Watanabe, A. Murata, H. Shigaki, K. Miyake, T. Ishimoto, M. Iwatsuki, S. Iwagami, N. Yoshida, E. Oki, K. Sakamaki, M. Nakao, and H. Baba.
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Journal Title
Clin Cancer Res
Volume: 20
Pages: 1114-1124
DOI
Related Report
Peer Reviewed
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[Journal Article] Aberrant methylation of LINE-1, SLIT2, MAL and IGFBP7 in non-small cell lung cancer2013
Author(s)
Suzuki M, Shiraishi K, Eguchi A, Ikeda K, Mori T, Yoshimoto K, Ohba Y, Ito Yamada T, Ito T, Baba Y, Baba H
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Journal Title
ONCOLOGY REPORTS
Volume: 29(4)
Pages: 1308-1314
DOI
Related Report
Peer Reviewed
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[Journal Article] Pyrosequencing Assay to Measure LINE-1 Methylation Level in Esophageal Squamous Cell Carcinoma2012
Author(s)
Iwagami S, Baba Y, Watanabe M, Shigaki H, Miyake K, Ida S, Nagai Y, Ishimoto T, Iwatsuki M, Sakamoto Y, Miyamoto Y, Baba H.
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Journal Title
Annals of Surgical Oncology
Volume: 19(8)
Pages: 2726-32
DOI
Related Report
Peer Reviewed
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