| Project/Area Number |
23689066
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University (2012-2013)
The University of Tokyo (2011) |
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Principal Investigator |
IMAI YUUKI 愛媛大学, プロテオサイエンスセンター, 教授 (10423873)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 骨関節疾患 / エピゲノム / 軟骨細胞 / 破骨細胞 / クロマチン / Aire / 関節リウマチ |
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| Research Abstract |
To clarify regulatory mechanism on epigenetics in bone and joint diseases, we identified Aire as a novel regulator in chondrocyte differentiation using gene expression microarray. Aire possesses PHD domain, which recognizes hypomethylated lysine residues of histone proteins. As the results of analyses of molecular function, Aire can facilitate chondrocyte differentiation through regulating status of histone H3K4me2 in promoter region of Bmp-2 (Si et al BBRC 2013).
In terms of osteoclasts, which resorb bone matrix, we tried to figure out dynamic chromatin remodeling during osteoclastogenesis using DNase-seq. Based on the combined information from gene expression and DNase-seq, we successfully identified novel transcription factors in osteoclastogenesis (Inoue et al. JBMR 2014 in press).
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