egulatory roles of macrophages in choroidal neovascular diseases
| Project/Area Number |
23689071
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Kyushu University |
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Principal Investigator |
TAKEDA Atsunobu 九州大学, 医学(系)研究科(研究院), 助教 (40560313)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2011: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | 脈絡膜血管新生 / マクロファージ / 網膜色素上皮細胞 / サイトカイン / 網膜下血管新生 / 眼炎症 / 炎症 / DAMP / TLR / IL-27 / VEGF / CNV |
|---|
| Research Abstract |
The purpose of this study is to clarify regulatory mechanism of macrophages in choroidal neovascular diseases. We show that IL-27, a regulatory cytokine, inhibited VEGFA production in macrophage in vitro. IL-27 also suppressed CNV by VEGFA reduction but not macrophage recruitment in vivo. M2 macrophage (M2) is reported as a source of VEGFA in CNV, indicating that IL-27 might regulate M2 differentiation and/or activation in CNV. Next, we focused on HSP70, an endogenous ligand for Toll-like receptor (TLR) 4/TLR2. HSP70, located in the cytosol and the nucleus of various kinds of cells, is released in response to cellular stress. Although M2 promotes cancer-associated fibrosis by activation of TLR4 signaling, subretinal fibrosis was suppressed by intraocular injection of HSP70 through induction of IL-10, an anti-inflammatory cytokine, via TLR4/TLR2 signaling. The induction of IL-10 was dependent on RPE, but not macrophages, suggesting that RPE is also important for the regulation.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Use of a comprehensive polymerase chain reaction system for diagnosis of ocular infectious diseases2013
Author(s)
Sugita S, Ogawa M, Shimizu N, Morio T, Ohguro N, Nakai K, Maruyama K, Nagata K, Takeda A, Usui Y, Sonoda KH, Takeuchi M, Mochizuki M
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Journal Title
Ophthalmology
Volume: Vol. 120, No. 9
Issue: 9
Pages: 1761-1768
DOI
Related Report
Peer Reviewed
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[Journal Article] Dynamic increase in extracellular ATP accelerates photoreceptor apoptosis via ligation of P2RX7 in subretinal hemorrhage2013
Author(s)
Notomi S, Hisatomi T, Murakami Y, Terasaki H, Sonoda S, Asato R, Takeda A, Ikeda Y, Enaida H, Sakamoto T, Ishibashi T
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Journal Title
Plos One
Volume: Vol. 8, No. 1(1371/journal.pone.0053338)
Related Report
Peer Reviewed
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[Presentation] Pivotal roles of P2RX7 in the induction of Th1 and Th17 responses in experimental autoimmune uveitis.2014
Author(s)
Takeda A, Yoshimura T, Hirakawa S, Hisatomi T, Ikeda Y, Enaida H, Oshima Y, Sonoda KH, Ishibashi T
Organizer
The Association for Research in Vision and Ophthalmology 2014.
Place of Presentation
Orlando, FL, USA
Related Report
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[Presentation] Pivotal roles of EBI3 for the initiation and maintenance of experimental autoimmune uveitis.2013
Author(s)
Takeda A, Yoshimura T, Hasegawa E, Yamada H, Hirakawa S, Hisatomi T, Ikeda Y, Yoshida H, Sonoda KH, Ishibashi T
Organizer
The Association for Research in Vision and Ophthalmology 2013.
Place of Presentation
Seatle, WA, USA
Related Report
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[Presentation] Long-term efficacy of infliximab maintenance therapy in refractory uveitis of Behcet’s disease at Kyushu University Hospital: a 24-month follow-up study.
Author(s)
Takeda A, Fukuhara T, Hijioka H, Hikita S, Yoshimura T, Akahoshi M, Niiro H, Tsukamoto H, Horiuchi T, Kawano Y, Sonoda KH, Ishibashi T
Organizer
The 6th joint meeting of China- Japan-Korea Ophthalmologists.
Place of Presentation
Xiamen, China
Related Report
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