| Budget Amount *help |
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Research Abstract |
In order to develop new therapeutic strategies for cutaneous ulcers, way for direct conversion of fibroblasts to keratinocytes are searched. Direct transdifferentiation to keratinocytes may enable de novo epithelialization from bottom of ulcers. Using microarrays and bioinformatic analysises, candidates factors for direct conversion to keratinocytes are selected. We picked up 55 transcription factors and 30 micro RNAs as candidates. Using lentiviral vectors, these factors are transduced to primary fibroblasts and obtained cells similar to keratinocytes in its form and proliferation kinetics.
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