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Searching way for direct conversion of mesenchymal cells to keratinocytes

Research Project

Project/Area Number 23689073
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Plastic surgery
Research InstitutionKyorin University

Principal Investigator

KURITA Masakazu  杏林大学, 医学部, 助教 (20424111)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywords直接転換 / ケラチノサイト / 線維芽細胞 / 創傷治癒 / リプログラミング / 遺伝子導入 / 間葉上皮移行 / 上皮化 / 間葉上皮転換 / マイクロRNA / 皮膚潰瘍 / 皮膚線維芽細胞 / 脂肪幹細胞
Research Abstract

In order to develop new therapeutic strategies for cutaneous ulcers, way for direct conversion of fibroblasts to keratinocytes are searched. Direct transdifferentiation to keratinocytes may enable de novo epithelialization from bottom of ulcers. Using microarrays and bioinformatic analysises, candidates factors for direct conversion to keratinocytes are selected. We picked up 55 transcription factors and 30 micro RNAs as candidates. Using lentiviral vectors, these factors are transduced to primary fibroblasts and obtained cells similar to keratinocytes in its form and proliferation kinetics.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (12 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (10 results) (of which Invited: 1 results)

  • [Journal Article] Extravascular injection of sclerotic agents does not cause detrimental influences even on tiny vessels of rat. An experimental implication for percutaneous sclerotherapy of arteriovenous malformations2012

    • Author(s)
      Sato Daisuke, Masakazu Kurita, et al
    • Journal Title

      Eur J Vase Endovasc Surg

      Volume: (In press) Issue: 1 Pages: 73-76

    • DOI

      10.1016/j.ejvs.2012.04.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential expression of wound fibrotic factors between facial and trunk dermal fibroblasts

    • Author(s)
      Masakazu Kurita, et al
    • Journal Title

      Connective Tissue Research

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Searching the way of direct conversion of fibroblasts into keratinocytes for the treatment of cutaneous ulcers2014

    • Author(s)
      Kurita M, Eto H, Suga H, Takushima A, Harii K
    • Organizer
      Cira International Symposium 2014
    • Place of Presentation
      Center for Learning and Innovation (CLI), Takeda Pharmaceutical Company, Ltd., Osaka, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] ケラチノサイトに特異的なmicro RNAの同定2014

    • Author(s)
      栗田昌和、江藤ひとみ、多久嶋亮彦、波利井清紀
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代、福島県
    • Related Report
      2013 Final Research Report
  • [Presentation] earching the way of direct conversion of fibroblasts into keratinocytes for the treatment of cutaneous ulcers.2014

    • Author(s)
      Kurita M, Eto H, Suga H, Takushima A, Harii K.
    • Organizer
      Cira International Symposium 2014
    • Place of Presentation
      Takeda Pharmaceutical Company, Ltd., Osaka, Japan.
    • Related Report
      2013 Annual Research Report
  • [Presentation] 皮膚潰瘍上皮化促進を目的とした線維芽細胞からケラチノサイトへの直接転換のこころみ2013

    • Author(s)
      栗田昌和、江藤ひとみ、佐藤卓志、菅浩隆、多久嶋亮彦、波利井清紀
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター、新潟県
    • Related Report
      2013 Final Research Report
  • [Presentation] 皮膚潰瘍上皮化促進を目的とした線維芽細胞からケラチノサイトへの直接転換のこころみパネルディスカッション1創傷治癒の分子メカニズム2013

    • Author(s)
      栗田昌和、江藤ひとみ、菅浩隆、多久嶋亮彦、波利井清紀
    • Organizer
      第5回日本創傷外科学会総会・学術集会
    • Place of Presentation
      ホテルグランヴィア京都、京都府
    • Related Report
      2013 Final Research Report
  • [Presentation] Searching the way of direct conversion of fibroblasts into keratinocytes for the treatment of cutaneous ulcers2013

    • Author(s)
      Kurita M Eto H, Suga H, Takushima A, Harii K
    • Organizer
      Cira International Symposium 2013
    • Place of Presentation
      Kyoto University, Clock tower centennial hall, Kyoto, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 皮膚潰瘍上皮化促進を目的とした線維芽細胞からケラチノサイトへの直接転換のこころみ2013

    • Author(s)
      栗田昌和、江藤ひとみ、菅浩隆、多久嶋亮彦、波利井清紀
    • Organizer
      第5回日本創傷外科学会総会・学術集会
    • Place of Presentation
      ホテルグランヴィア京都、京都府
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 皮膚潰瘍上皮化促進を目的とした線維芽細胞からケラチノサイトへの直接転換のこころみ2013

    • Author(s)
      栗田昌和、江藤ひとみ、佐藤卓志、菅浩隆、多久嶋亮彦、波利井清紀
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      新潟コンベンションセンター、新潟県
    • Related Report
      2013 Annual Research Report
  • [Presentation] Searching the way of direct conversion of fibroblasts into keratinocytes for the treatment of cutaneous ulcers.2013

    • Author(s)
      Kurita M et al.
    • Organizer
      Cira International Symposium 2013
    • Place of Presentation
      Kyoto University, Kyoto
    • Related Report
      2012 Annual Research Report
  • [Presentation] ケラチノサイトに特異的なmicro RNAの同定2012

    • Author(s)
      栗田昌和ほか
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代、福島県
    • Related Report
      2012 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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