Searching way for direct conversion of mesenchymal cells to keratinocytes
Project/Area Number |
23689073
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Single-year Grants |
Research Field |
Plastic surgery
|
Research Institution | Kyorin University |
Principal Investigator |
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
Keywords | 直接転換 / ケラチノサイト / 線維芽細胞 / 創傷治癒 / リプログラミング / 遺伝子導入 / 間葉上皮移行 / 上皮化 / 間葉上皮転換 / マイクロRNA / 皮膚潰瘍 / 皮膚線維芽細胞 / 脂肪幹細胞 |
Research Abstract |
In order to develop new therapeutic strategies for cutaneous ulcers, way for direct conversion of fibroblasts to keratinocytes are searched. Direct transdifferentiation to keratinocytes may enable de novo epithelialization from bottom of ulcers. Using microarrays and bioinformatic analysises, candidates factors for direct conversion to keratinocytes are selected. We picked up 55 transcription factors and 30 micro RNAs as candidates. Using lentiviral vectors, these factors are transduced to primary fibroblasts and obtained cells similar to keratinocytes in its form and proliferation kinetics.
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Report
(4 results)
Research Products
(12 results)