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Establishment of periodontal tissue regeneration method by using of novel PDLSC cell surface marker and PDL marker

Research Project

Project/Area Number 23689077
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionKyushu University

Principal Investigator

WADA Naohisa  九州大学, 大学病院, 講師 (60380466)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywords歯根膜組織 / 間葉系幹細胞 / 再生 / 歯根膜 / 再生医療
Research Abstract

We found that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properies in human periodontal ligament (PDL) cells, and Wnt5a can induce collagen fibrillogenesis which is main component of PDL tissue, by human PDL cells (HPDLC). Sema3A-overexpressing HPDLC exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes, and upregulated the expression of stem cell markers. Meanwhile, Wnt5a suppressed osteoblastic differentiation of HPDLC, while it significantly enhanced proliferation and migration of HPDLC, and PDL-related gene expression and collagen production in HPDLC. Both knockdown of periostin gene expression and inhibition of TGF-beta1 function suppressed the Wnt5a-induced PDL-related gene expression and collagen production. Although further studies are required to clarify the precise functional mechanism of both factors, Sema3A and Wnt5a can be factors with potential to regenerate PDL tissue efficiently.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (29 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 8 results) Presentation (16 results) (of which Invited: 1 results) Book (3 results)

  • [Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells2014

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
    • Journal Title

      Stem Cells Dev

      Volume: (Epub ahead of print) Issue: 18 Pages: 2225-2236

    • DOI

      10.1089/scd.2013.0405

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Periodontal Ligament Derived Stem Cells Exhibit the Capacity for Long-Term Survival, Self-Renewal and Regeneration of Multiple Tissue Types in Vivo2014

    • Author(s)
      Menicanin D, Mrozik M, Wada N, Marino V, Shi S, Bartold PM, Gronthos S
    • Journal Title

      Stem Cells Dev

      Volume: 23(9) Issue: 9 Pages: 1001-1011

    • DOI

      10.1089/scd.2013.0490

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration of the periodontium for preservation of the damaged tooth2014

    • Author(s)
      Maeda H, Tomokiyo A, Wada N, Koori K, Kawachi G, Akamine A
    • Journal Title

      Histol Histopathol

      Volume: (Epub ahead of print)

    • URL

      http://www.hh.um.es/Reviews-proofs/11-489-manuscript.pdf

    • Related Report
      2013 Final Research Report
  • [Journal Article] Regeneration of the periodontium for preservation of the damaged tooth.2014

    • Author(s)
      Maeda H, Tomokiyo A, Wada N, Koori K, Kawachi G, Akamine A.
    • Journal Title

      Histol Histopathol.

      Volume: [Epub ahead of print]

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunomodulatory Effects of Stem Cells2013

    • Author(s)
      Wada N, Gronthos S, Bartold PM
    • Journal Title

      Periodontol 2000

      Volume: 63(1) Issue: 1 Pages: 198-216

    • DOI

      10.1111/prd.12024

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prospective Potency of TGF-β1 on Maintenance and Regeneration of Periodontal Tissue2013

    • Author(s)
      Maeda H, Wada N, Tomokiyo A, Monnouchi S, Akamine A
    • Journal Title

      Int Rev Cell Mol Biol

      Volume: 304 Pages: 283-367

    • DOI

      10.1016/b978-0-12-407696-9.00006-3

    • ISBN
      9780124076969
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells.2013

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A.
    • Journal Title

      Stem Cells Dev.

      Volume: [Epub ahead of print]

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells.2013

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A.
    • Journal Title

      Stem Cells Dev

      Volume: [Epub ahead of print]

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prospective Potency of TGF-β1 on Maintenance and Regeneration of Periodontal Tissue.2013

    • Author(s)
      Maeda H, Wada N, Tomokiyo A, Monnouchi S, Akamine A
    • Journal Title

      Int Rev Cell Mol Biol.

      Volume: 304 Issue: 6 Pages: 283-367

    • DOI

      10.11607/jomi.te26

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Promise of periodontal ligament stem cells in regeneration of periodontium2011

    • Author(s)
      Maeda H, Tomokiyo A, Fujii S, Wada N, Akamine A
    • Journal Title

      Stem Cell Res Ther

      Volume: 2 Issue: 4 Pages: 33-33

    • DOI

      10.1186/scrt74

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Wnt5aはTGFβ1を介してヒト歯根膜細胞のコラーゲン線維形成を促進する2014

    • Author(s)
      長谷川大学、和田尚久、前田英史、吉田晋一郎、門野内聡、御手洗裕美、濱野さゆり、祐田明香、赤峰昭文
    • Organizer
      第140回日本歯科保存学会春季学術大会
    • Place of Presentation
      滋賀県立芸術劇場(大津市)
    • Related Report
      2013 Final Research Report
  • [Presentation] The Effects of Wnt5a on Human Periodontal Ligament Cells2014

    • Author(s)
      Hasegawa D, Wada N, Maeda H, Yoshida S, Monnouchi S, Koori K, Hamano S, Mitarai H, Akamine A
    • Organizer
      Kyudai Oral Bioscience 2014 - 8th International Symposium -
    • Place of Presentation
      Fukuoka Recent Hotel, Fukuoka, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Wnt5aはTGFβ1を介してヒト歯根膜細胞のコラーゲン線維形成を促進する2014

    • Author(s)
      長谷川大学、和田尚久、前田英史、吉田晋一郎、門野内聡、 御手洗裕美、濱野さゆり、祐田明香、赤峰昭文
    • Organizer
      第140回日本歯科保存学会春季学術大会
    • Place of Presentation
      滋賀県立芸術劇場びわ湖ホール
    • Related Report
      2013 Annual Research Report
  • [Presentation] The Effects of Wnt5a on Human Periodontal Ligament Cells.2014

    • Author(s)
      Hasegawa D, Wada N, Maeda H, Yoshida S, Monnouchi S, Koori K, Hamano S, Mitarai H, Akamine A.
    • Organizer
      Kyudai Oral Bioscience 2014 - 8th International Symposium -
    • Place of Presentation
      Fukuoka Recent Hotel, Fukuoka, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] 再生医療における歯原性幹細胞の可能性2013

    • Author(s)
      和田尚久
    • Organizer
      日本再生歯科医学会2013シンポジウム「よみがえる歯髄・歯根膜・根尖歯周組織」
    • Place of Presentation
      西日本総合展示場AIMビル(北九州市)
    • Year and Date
      2013-02-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Wnt5aがヒト歯根膜細胞に及ぼす影響について2013

    • Author(s)
      長谷川大学、和田尚久、前田英史、友清淳、門野内聡、郡勝明、吉田晋一郎、寺松陽子、濱野さゆり、祐田明香、杉井英樹、赤峰昭文
    • Organizer
      第139回日本歯科保存学会秋季学術大会
    • Place of Presentation
      秋田県総合生活文化会館(秋田市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Conversion of periodontal ligament cells into multipotent stem-like cells2013

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
    • Organizer
      91st General Session & Exhibition of the IADR
    • Place of Presentation
      Seattle, Washington
    • Related Report
      2013 Final Research Report
  • [Presentation] Wnt5aがヒト歯根膜細胞に及ぼす影響について.2013

    • Author(s)
      長谷川大学、和田尚久、前田英史、友清淳、門野内聡、郡勝明、吉田晋一郎、寺松陽子、濱野さゆり、祐田明香、杉井英樹、赤峰昭文.
    • Organizer
      第139回日本歯科保存学会秋季学術大会
    • Place of Presentation
      秋田県総合生活文化会館(秋田市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Conversion of periodontal ligament cells into multipotent stem-like cells.2013

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
    • Organizer
      91st General Session & Exhibition of the IADR
    • Place of Presentation
      Seattle, Washington
    • Related Report
      2012 Annual Research Report
  • [Presentation] Conversion of periodontal ligament cells into multipotent stem-like cells.2013

    • Author(s)
      Naohisa Wada
    • Organizer
      91st General Session & Exhibition of the IADR
    • Place of Presentation
      Convention Center in Seattle
    • Related Report
      2011 Annual Research Report
  • [Presentation] Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A2012

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
    • Organizer
      The Australian Health and Medical Research Congress 2012
    • Place of Presentation
      Adelaide Convention Centre, Adelaide, Australia
    • Related Report
      2013 Final Research Report
  • [Presentation] 分化能の異なるヒト歯根膜細胞クローンの単離及びキャラクタライゼーション2012

    • Author(s)
      長谷川大学、和田尚久、前田英史、藤井慎介、郡勝明、友清淳、門野内聡、河野清美、山本直秀、寺松陽子、濱野さゆり、祐田明香、赤峰昭文
    • Organizer
      第137回日本歯科保存学会秋季学術大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 分化能の異なるヒト歯根膜細胞クローンの単離及びキャラクタライゼーション.2012

    • Author(s)
      長谷川大学、和田尚久、前田英史、藤井慎介、郡勝明、友清淳、門野内聡、河野清美、山本直秀、寺松陽子、濱野さゆり、祐田明香、赤峰昭文.
    • Organizer
      第137回日本歯科保存学会秋季学術大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A.2012

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A.
    • Organizer
      The Australian Health and Medical Research Congress 2012
    • Place of Presentation
      Adelaide Convention Centre, Adelaide, Australia
    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
  • [Presentation] 分化能の異なるヒト歯根膜細胞クローンの単離及びキャラクタライゼーション2012

    • Author(s)
      長谷川大学
    • Organizer
      第137回日本歯科保存学会秋季学術大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 再生医療における歯原性幹細胞の可能性

    • Author(s)
      和田尚久
    • Organizer
      日本再生歯科医学会2013シンポジウム「よみがえる歯髄・歯根膜・根尖歯周組織」
    • Place of Presentation
      西日本総合展示場AIMビル(北九州市)
    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Invited
  • [Book] Differentiation of Periodontal Ligament Stem /Progenitor Cells : Roles of TGF-β1. In : Stem Cells and Cancer Stem Cells, volume 4, Therapeutic Applications in Disease and Injury. Hayat MA (ed)(Chapter 5)2012

    • Author(s)
      Maeda H, Fujii S, Monnouchi S, Wada N, Akamine A
    • Publisher
      Springer, Heidelberg, Germany
    • Related Report
      2013 Final Research Report
  • [Book] Periodontal ligament stem cells. In : Stem Cells in Clinic and Research. Gholamrezanezhad A (ed)(Chapter 25)2011

    • Author(s)
      Maeda H, Wada N, Fujii S, Tomokiyo A, Akamine A
    • Publisher
      InTech, Rijeka, Croatia
    • Related Report
      2013 Final Research Report
  • [Book] Periodontal ligament stem cells. In: Stem Cells in Clinic and Research2011

    • Author(s)
      Hidefumi Maeda
    • Publisher
      InTech, Rijeka, Croatia
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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