Establishment of periodontal tissue regeneration method by using of novel PDLSC cell surface marker and PDL marker
Project/Area Number |
23689077
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Conservative dentistry
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Research Institution | Kyushu University |
Principal Investigator |
WADA Naohisa 九州大学, 大学病院, 講師 (60380466)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
|
Keywords | 歯根膜組織 / 間葉系幹細胞 / 再生 / 歯根膜 / 再生医療 |
Research Abstract |
We found that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properies in human periodontal ligament (PDL) cells, and Wnt5a can induce collagen fibrillogenesis which is main component of PDL tissue, by human PDL cells (HPDLC). Sema3A-overexpressing HPDLC exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes, and upregulated the expression of stem cell markers. Meanwhile, Wnt5a suppressed osteoblastic differentiation of HPDLC, while it significantly enhanced proliferation and migration of HPDLC, and PDL-related gene expression and collagen production in HPDLC. Both knockdown of periostin gene expression and inhibition of TGF-beta1 function suppressed the Wnt5a-induced PDL-related gene expression and collagen production. Although further studies are required to clarify the precise functional mechanism of both factors, Sema3A and Wnt5a can be factors with potential to regenerate PDL tissue efficiently.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells2014
Author(s)
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
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Journal Title
Stem Cells Dev
Volume: (Epub ahead of print)
Issue: 18
Pages: 2225-2236
DOI
Related Report
Peer Reviewed
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[Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells.2013
Author(s)
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A.
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Journal Title
Stem Cells Dev.
Volume: [Epub ahead of print]
Related Report
Peer Reviewed
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[Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells.2013
Author(s)
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A.
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Journal Title
Stem Cells Dev
Volume: [Epub ahead of print]
Related Report
Peer Reviewed
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[Presentation] The Effects of Wnt5a on Human Periodontal Ligament Cells2014
Author(s)
Hasegawa D, Wada N, Maeda H, Yoshida S, Monnouchi S, Koori K, Hamano S, Mitarai H, Akamine A
Organizer
Kyudai Oral Bioscience 2014 - 8th International Symposium -
Place of Presentation
Fukuoka Recent Hotel, Fukuoka, Japan
Related Report
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[Presentation] The Effects of Wnt5a on Human Periodontal Ligament Cells.2014
Author(s)
Hasegawa D, Wada N, Maeda H, Yoshida S, Monnouchi S, Koori K, Hamano S, Mitarai H, Akamine A.
Organizer
Kyudai Oral Bioscience 2014 - 8th International Symposium -
Place of Presentation
Fukuoka Recent Hotel, Fukuoka, Japan
Related Report
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[Presentation] Conversion of periodontal ligament cells into multipotent stem-like cells2013
Author(s)
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
Organizer
91st General Session & Exhibition of the IADR
Place of Presentation
Seattle, Washington
Related Report
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[Presentation] Conversion of periodontal ligament cells into multipotent stem-like cells.2013
Author(s)
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
Organizer
91st General Session & Exhibition of the IADR
Place of Presentation
Seattle, Washington
Related Report
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[Presentation] Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A2012
Author(s)
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
Organizer
The Australian Health and Medical Research Congress 2012
Place of Presentation
Adelaide Convention Centre, Adelaide, Australia
Related Report
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[Presentation] Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A.2012
Author(s)
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A.
Organizer
The Australian Health and Medical Research Congress 2012
Place of Presentation
Adelaide Convention Centre, Adelaide, Australia
Related Report
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